CAJ | 학술논문

目的探讨原发性肝癌患者外周血自然杀伤(NK)细胞受体NKG2D表达的变化,并分析其对NK细胞杀伤活性的影响.方法用流式细胞仪分析20例原发性肝癌、23例乙型肝炎后肝硬化、20例慢性乙型肝炎及20名健康者的外周血NK细胞数量及其NKG2D的表达情况,并用酶标仪检测各组样本NK细胞的细胞毒活性.结果原发性肝癌患者的NK细胞杀伤率、NK细胞NKG2D的表达率(中位数)、NKG2D+NK细胞数(中位数)、NK细胞中NKG2D的表达水平(中位数)及NK细胞数[(25±7)%、6%、0.7×107/L、15、(1.1±0.6)×108/L]均明显低于正常组[(63±7)%、36%、8.3×107/L、116、(27±1.1)×108/L]和乙肝组[(41±8)%、16%、2.8×107/L,49、(1.9±1.1)×108/L],差异均有统计学意义(均P＜0.05);与肝硬化组[(29±10)%、7%、0.6×107/L、29、(1.5±1.2)×108/L]比较也略低,但只有NK细胞NKG2D的表达水平两组差异有统计学意义(P＜0.05),其余两组间差异均无统计学意义(均P＞0.05).NK细胞的活性与NK细胞数、NK细胞NKG2D的表达率、NKG2D+NK细胞数、NK细胞NKG2D的表达水平均呈正相关关系(r=0.657、0.770、0.927、0.734,均P＜0.01).结论原发性肝癌患者外周血NK细胞活性及其细胞表面NKG2D的表达明显降低,NKG2D的表达与NK细胞的活性密切相关,提高NK细胞表面NKG2D的表达水平可为原发性肝癌的过继免疫治疗开创新的思路.
목적 탐토원발성간암환자외주혈자연살상(NK)세포수체NKG2D표체적변화,병분석기대NK세포살상활성적영향.방법 용류식세포의분석20례원발성간암、23례을형간염후간경화、20례만성을형간염급20명건강자적외주혈NK세포수량급기NKG2D적표체정황,병용매표의검측각조양본NK세포적세포독활성.결과 원발성간암환자적NK세포살상솔、NK세포NKG2D적표체솔(중위수)、NKG2D+NK세포수(중위수)、NK세포중NKG2D적표체수평(중위수)급NK세포수[(25±7)%、6%、0.7×107/L、15、(1.1±0.6)×108/L]균명현저우정상조[(63±7)%、36%、8.3×107/L、116、(27±1.1)×108/L]화을간조[(41±8)%、16%、2.8×107/L,49、(1.9±1.1)×108/L],차이균유통계학의의(균P＜0.05);여간경화조[(29±10)%、7%、0.6×107/L、29、(1.5±1.2)×108/L]비교야략저,단지유NK세포NKG2D적표체수평량조차이유통계학의의(P＜0.05),기여량조간차이균무통계학의의(균P＞0.05).NK세포적활성여NK세포수、NK세포NKG2D적표체솔、NKG2D+NK세포수、NK세포NKG2D적표체수평균정정상관관계(r=0.657、0.770、0.927、0.734,균P＜0.01).결론 원발성간암환자외주혈NK세포활성급기세포표면NKG2D적표체명현강저,NKG2D적표체여NK세포적활성밀절상관,제고NK세포표면NKG2D적표체수평가위원발성간암적과계면역치료개창신적사로.
Objective To investigate the expression of NK cells receptor NKG2D from peripheral blood in patients with primary hepatic carcinoma and the relationship between NKG2D expression and cytotoxicity of NK cells.Methods Flow cytometry was used to determine the number of NK cells and the expression of NK cells receptor NKG2D from peripheral blood in patients with primary hepatic carcinoma (20 cases) ,hepatitis B cirrhosis (23 cases ),hepatitis B ( 20 cases ) and healthy control ( 20 cases ).The microplate reader was used to detect cytotoxicity of NK cells in all cases.Results Among killing rate of NK cell for K562 cell,the expression rate of NKG2D in NK cells,the number of NKG2D+ NK cells,NKG2D expression level of NK cells and the number of NK cells,the liver cancer group[(25 ±7)% ,6% ,0.7 × 107/L,15,(1.1 ± 0.6) × 108/L]decreased significantly as compared with the healthy group [(63 ± 7 )% ,36%,8.3 ×107/L,116,(2.7 ±1.1) × 108/L]and the hepatitis B group [(41 ± 8)%,16%,2.8 × 107/L,49,(1.9 ± 1.1 ) × 108/L](P ＜ 0.05 ) ;and there was a slight decrease as compared with the hepatitis B cirrhosis group[(29 ± 10)% ,7% ,0.6 × 107/L,29,(1.5 ± 1.2)× 108/L](all P ＞0.05 except NKG2D expression level of NK cells P ＜0.05).The activity of NK cells showed a obvious positive correlation with the number of NK cell and the positive rate of NKG2D in NK cells,the number of NKG2D+ NK cells and NKG2D expression level of NK cells (r=0.657,0.770,0.927,0.734,all P＜0.01).Conclusion The cytotoxicity and the NKG2D expression of NK cells decreased significantly from peripheral blood in patients with primary hepatic carcinoma.The activity of NK cells was closely related to the NKG2D expression level of NK cells.Enhancing the NKG2D expression level of NK cell may provide a new idea for adoptive immunotherapy of primary hepatic carcinoma.