CAJ | 학술논문

本文研究了杜仲中具有生物活性的环烯醚萜类化合物的最佳提取工艺.采用正交设计表L16(45),以提取时间、提取温度、提取次数、提取剂A的浓度及料液比为因素进行实验优化.桃叶珊瑚甙的含量由分光光度法测得,京尼平甙酸和京尼平甙的含量通过高效液相色谱法测定.根据试验结果,提取桃叶珊瑚甙的最佳工艺是:70℃下提取三次,每次0.5小时,溶剂A的浓度为80%,料液比为1:12.而京尼平甙和京尼平甙酸的最佳提取工艺为:在70℃下提取一次,提取时间为1小时,溶剂A的浓度为50%,料液比也为1:12.在此工艺条件下,选取了较佳的提取原料.实验表明在实际应用中,应根据提取目标和原材料选择较好的提取工艺.
본문연구료두중중구유생물활성적배희미첩류화합물적최가제취공예.채용정교설계표L16(45),이제취시간、제취온도、제취차수、제취제A적농도급료액비위인소진행실험우화.도협산호대적함량유분광광도법측득,경니평대산화경니평대적함량통과고효액상색보법측정.근거시험결과,제취도협산호대적최가공예시:70℃하제취삼차,매차0.5소시,용제A적농도위80%,료액비위1:12.이경니평대화경니평대산적최가제취공예위:재70℃하제취일차,제취시간위1소시,용제A적농도위50%,료액비야위1:12.재차공예조건하,선취료교가적제취원료.실험표명재실제응용중,응근거제취목표화원재료선택교호적제취공예.
A optimum technology of extracting bioactive components named Iridoids in Eucommia ulmoides was investigated by orthogonal test in this paper. The orthogonal test table L16(45) was used with the factors of extracting time, temperature, extracting times, concentration of organic solvent A and ratio of solvent to material. The content of Aucubin was determined by spectrophotometry. And the concentration of Geniposide and Geniposidic acid were obtained by HPLC. According to the result of the test, the optimum technology for Aucubin was temperature 70℃,0.5 hour, three times, concentration of organic solvent A 80 % and the ratio of solvent to material 1: 12. As to Geniposidic acid and Geniposide they were, temperature 70℃,1 hour,extractingonce, concentration of organic solvent A 50%, the ratio of solvent to material 1: 12. We should choose the better technology according to our aim and material.