CAJ | 학술논문

背景:颈动脉窦存在压力感受器,是离子对血压调节最敏感的部位,在血压的调节中起到重要的作用,但不同离子通过颈动脉窦对血压的调节作用还不十分清楚.目的:观察用不同浓度、不同离子浸泡颈动脉窦后血压的变化.设计:自身对照实验.单位:解放军第四军医大学基础部教学实验中心.材料:实验于2000-12/2001-06在解放军第四军医大学基础部教学实验中心机能实验室完成.选取纯种新西兰兔18只,实验动物标准为二级,雌雄不限,体质量(2.0±0.2)kg,由解放军第四军医大学动物实验中心提供.方法:采用随机数字表法将白兔分为Na+组、K+组和Ca2+组,每组6只.将家兔麻醉后,气管插管,分离两侧颈动脉,一侧分离出颈动脉窦,另一侧进行血管插管并记录血压,用加液枪于颈动脉窦外分别由低浓度到高浓度滴加Na+、K+和Ca2+溶液,将颈动脉窦完全浸泡;记录每次加液前血压的基础值和加液后血压变化的峰值.主要观察指标:家兔颈动脉血压的基础值和加液后血压变化的峰值.结果:0.15,1.5 mol/L的高浓度Na+加液后血压为(97±12),(83±17)mm Hg,与基础值(106±14),(105±12)mm Hg比较有显著的降血压作用(t=2.946,P＜0.05);0.4 mol/L的高浓度K+有显著的降血压作用[(106±12),(64±13)mmHg,(t=13.496,P＜0.01)],其他浓度的K+降血压作用不明显(P＞0.05);0.07 mol/L的低浓度Ca2+加液后血压为(113±16)mm Hg,与基础值(103±12)mm Hg比较有显著的升血压作用(t=-3.627,P＜0.01).结论:Na+、K+和Ca2+可以通过直接作用于颈动脉窦调节血压.高浓度的Na+、K+具有降压作用,低浓度的Ca2+具有升压作用,这可能是血压调节的一种重要机制.
배경:경동맥두존재압력감수기,시리자대혈압조절최민감적부위,재혈압적조절중기도중요적작용,단불동리자통과경동맥두대혈압적조절작용환불십분청초.목적:관찰용불동농도、불동리자침포경동맥두후혈압적변화.설계:자신대조실험.단위:해방군제사군의대학기출부교학실험중심.재료:실험우2000-12/2001-06재해방군제사군의대학기출부교학실험중심궤능실험실완성.선취순충신서란토18지,실험동물표준위이급,자웅불한,체질량(2.0±0.2)kg,유해방군제사군의대학동물실험중심제공.방법:채용수궤수자표법장백토분위Na+조、K+조화Ca2+조,매조6지.장가토마취후,기관삽관,분리량측경동맥,일측분리출경동맥두,령일측진행혈관삽관병기록혈압,용가액창우경동맥두외분별유저농도도고농도적가Na+、K+화Ca2+용액,장경동맥두완전침포;기록매차가액전혈압적기출치화가액후혈압변화적봉치.주요관찰지표:가토경동맥혈압적기출치화가액후혈압변화적봉치.결과:0.15,1.5 mol/L적고농도Na+가액후혈압위(97±12),(83±17)mm Hg,여기출치(106±14),(105±12)mm Hg비교유현저적강혈압작용(t=2.946,P＜0.05);0.4 mol/L적고농도K+유현저적강혈압작용[(106±12),(64±13)mmHg,(t=13.496,P＜0.01)],기타농도적K+강혈압작용불명현(P＞0.05);0.07 mol/L적저농도Ca2+가액후혈압위(113±16)mm Hg,여기출치(103±12)mm Hg비교유현저적승혈압작용(t=-3.627,P＜0.01).결론:Na+、K+화Ca2+가이통과직접작용우경동맥두조절혈압.고농도적Na+、K+구유강압작용,저농도적Ca2+구유승압작용,저가능시혈압조절적일충중요궤제.
BACKGROUND: There are pressure sensors in carotid-sinus, which are very sensitive to blood pressure regulated by ions and play an important role in the regulation of blood pressure. But it is yet not very clear how different ions regulate the blood pressure through pressure sensors in carotidsinus.OBJECTIVE: To observe the effect of different ions at various concentrations outside the carotid-sinus.DESIGN: Self-control experiment.SETTING: Preclinical Experiment Center, Fourth Military Medical University of Chinese PLA.MATERIALS: The experiment was accomplished in the Preclinical Experiment Center, Fourth Military Medical University of Chinese PLA from December 2000 to June 2001. Totally 18 New Zealand pure strain rabbits were provided by the Aninal Experimenting Center of Fourth Military Medical University of Chinese PLA. They were standard grade Ⅱ, of either gender and body mass was (2.0±0.2) kg.METHODS: The rabbits were divided into Na+, K+ and Ca2+ groups according to random numbers, and each group consisted of 6 rabbits. After anaesthesia, tracheal intubatton was performed on the rabbit, and bilateral carotid arteries were separated with carotid-sinus separated on one side and vessel intubatton performed in the other side for blood pressure measurement. Then various concentrations of Na+, K+ and Ca2+ ions were added outside the carotid-sinus with the pipette to make the carotid-sinus completed immersed in the ion solutions. The basal blood pressure and the peak value after ions addition were recorded respectively.MAIN OUTCOME MEASURES: The basal blood pressure and the peak value after ions addition.RESULTS: After Na+ (0.15, 1.5 mol/L) was added the blood pressure was(97±12), (83±17) mm Hg. It was decreased significantly compared with the basal value (106±14), (105±12) mm Hg (t=2.946, P ＜ 0.05). K+ (0.4 mol/L)decreased the blood pressure significantly [(106±12), (64±13) mm Hg, (t=13.496, P ＜ 0.01)], but other concentrations of K+ were not effective. Ca2+(0.07 mol/L) increased the blood pressure to (113±16) mm Hg compared with the basal value (103±12) mm Hg (t=-3.627, P ＜ 0.01).CONCLUSION: Na+, K+ and Ca2+ regulate the blood pressure by acting on the carotid-sinus directly. High concentrations of Na+ and K+ possess the effect of decreasing the blood pressure, while high concentrations of Ca2+increases it, which may be an important mechanism of blood pressure regulation.