CAJ | 학술논문

采用大田研究方法,模拟5.0kJ·m~(-2)紫外辐射(UV-B,280～315nm)增强对灯盏花Erigeronbreviscapus附生、内生细菌数量、优势种群以及类黄酮、游离氨基酸、可溶性蛋白、淀粉和可溶性糖含量的影响.UV-B辐射极显著减少灯盏花苗期叶和花期根与叶附生细菌数量(p<0.01),显著减少灯盏花果熟期根与叶附生细菌数量(p<0.05).极显著减少灯盏花苗期根与叶和花期叶与茎及果熟期茎内生细菌的数量,果熟期根内生细菌数量显著增加.灯盏花附生细菌优势种群为芽孢杆菌Bacillus和欧文氏菌Erwinia,内生细菌优势种群为芽孢杆菌;UV-B辐射可导致灯盏花附生和内生细菌优势种群数目减少.UV-B辐射还会使灯盏花生理指标发生变化,直接导致灯盏花附生细菌数量的减少,可溶性糖、游离氨基酸和可溶性蛋白含量与附生细菌数量呈显著正相关(p<0.05).UV-B辐射增加灯盏花各部位类黄酮含量,间接影响灯盏花内生细菌数量,根类黄酮含量与内生细菌数量呈极显著正相关(p<0.01).
채용대전연구방법,모의5.0kJ·m~(-2)자외복사(UV-B,280～315nm)증강대등잔화Erigeronbreviscapus부생、내생세균수량、우세충군이급류황동、유리안기산、가용성단백、정분화가용성당함량적영향.UV-B복사겁현저감소등잔화묘기협화화기근여협부생세균수량(p<0.01),현저감소등잔화과숙기근여협부생세균수량(p<0.05).겁현저감소등잔화묘기근여협화화기협여경급과숙기경내생세균적수량,과숙기근내생세균수량현저증가.등잔화부생세균우세충군위아포간균Bacillus화구문씨균Erwinia,내생세균우세충군위아포간균;UV-B복사가도치등잔화부생화내생세균우세충군수목감소.UV-B복사환회사등잔화생리지표발생변화,직접도치등잔화부생세균수량적감소,가용성당、유리안기산화가용성단백함량여부생세균수량정현저정상관(p<0.05).UV-B복사증가등잔화각부위류황동함량,간접영향등잔화내생세균수량,근류황동함량여내생세균수량정겁현저정상관(p<0.01).
Field studies were conducted for analyzing dominant populations, quantities of epiphytic bacteria and endophytic bacteria, and the contents of flavonoid, free amino acid, soluble portion, soluble sugar and starch of Erigeron breviscapus with enhanced ul-traviolet-B radiation(5.0 kJ·m~(-2)). The quantities of the epiphytic bacteria in leaf of seedling, root and leaf in flowering stages were decreased significantly with UV-B radiation(p<0.01), as well as in root and leaf of maturity stage(p<0.05). UV-B radiation significantly reduced the quantities of the endophytic bacteria in root and leaf of seedling, leaf and stem in flowering stages, whilst significantly increased in root of maturity stage. The dominant populations of epiphytic bacteria were Bacillus and Erwinia. The dominant population of endophytic bacteria was Bacillus. UV-B radiation decreased the amount of the dominant populations of epiphytic and endophytic bacteria. The quantities of epiphytic bacteria decreased due to changes of physiological metabolish with UV-B radiation. Significantly positive relationships were observed between the contents of free amino acid, soluble portion, soluble sugar and the quantity of epiphytic bacteria(p<0.05). UV-B radiation increased flavonoid contents in each parts of Erigeron breviscapus. Significant positive correlation was observed between flavonoid contents and the quantity of endophytic(p<0.01).