中国生物医学工程学报
中國生物醫學工程學報
중국생물의학공정학보
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
1期
144-149
,共6页
褚薛慧%施晓雷%顾劲扬%冯章启%顾忠泽%丁义涛
褚薛慧%施曉雷%顧勁颺%馮章啟%顧忠澤%丁義濤
저설혜%시효뢰%고경양%풍장계%고충택%정의도
生物人工肝%壳聚糖%肝细胞%纳米纤维电纺膜
生物人工肝%殼聚糖%肝細胞%納米纖維電紡膜
생물인공간%각취당%간세포%납미섬유전방막
bioartificial liver%chitosan%hepatocyte%nanofiber scaffold
生物人工肝作为肝功能衰竭的一种有效支持手段,近年来得到很大的进展,但如何长期维持其中肝细胞的活性及功能一直是困扰人们的难题.纳米生物材料的应用为这一问题的解决提供了可能的方案.用静电纺丝的方法将壳聚糖制备成纳米纤维电纺膜,同时采用经典的两步原位胶原酶灌注法分离大鼠肝细胞,观察肝细胞在纳米纤维电纺膜表面的形态,同时检测其活性和功能.肝细胞在壳聚糖纳米纤维电纺膜表面展示了良好的活性,并能与支架材料紧密结合.其尿素合成、蛋白分泌及细胞色素P450的活性均为对照组的1.5~2倍,糖原合成也较对照组有明显增强.壳聚糖纳米纤维电纺膜能促进肝细胞黏附,同时具有良好的生物相容性,从而促进肝细胞的功能,是生物反应器中肝细胞黏附介质的理想材料.
生物人工肝作為肝功能衰竭的一種有效支持手段,近年來得到很大的進展,但如何長期維持其中肝細胞的活性及功能一直是睏擾人們的難題.納米生物材料的應用為這一問題的解決提供瞭可能的方案.用靜電紡絲的方法將殼聚糖製備成納米纖維電紡膜,同時採用經典的兩步原位膠原酶灌註法分離大鼠肝細胞,觀察肝細胞在納米纖維電紡膜錶麵的形態,同時檢測其活性和功能.肝細胞在殼聚糖納米纖維電紡膜錶麵展示瞭良好的活性,併能與支架材料緊密結閤.其尿素閤成、蛋白分泌及細胞色素P450的活性均為對照組的1.5~2倍,糖原閤成也較對照組有明顯增彊.殼聚糖納米纖維電紡膜能促進肝細胞黏附,同時具有良好的生物相容性,從而促進肝細胞的功能,是生物反應器中肝細胞黏附介質的理想材料.
생물인공간작위간공능쇠갈적일충유효지지수단,근년래득도흔대적진전,단여하장기유지기중간세포적활성급공능일직시곤우인문적난제.납미생물재료적응용위저일문제적해결제공료가능적방안.용정전방사적방법장각취당제비성납미섬유전방막,동시채용경전적량보원위효원매관주법분리대서간세포,관찰간세포재납미섬유전방막표면적형태,동시검측기활성화공능.간세포재각취당납미섬유전방막표면전시료량호적활성,병능여지가재료긴밀결합.기뇨소합성、단백분비급세포색소P450적활성균위대조조적1.5~2배,당원합성야교대조조유명현증강.각취당납미섬유전방막능촉진간세포점부,동시구유량호적생물상용성,종이촉진간세포적공능,시생물반응기중간세포점부개질적이상재료.
As a useful means of temporary liver support for patients awaiting liver transplantation, bioartificial liver (BAL) has made great progress in recent years. However, how to enhance the cell attachment and promote the liver functions of hepatocyte remains one of the major chanllenges in BAL. The application of nanofiber scaffold may afford possible solution to this problem. A new chitosan nanofiber scaffold was designed via electrospinning, and the rat hepatocytes were harvested by a two-step in situ collagenase perfusion procedure. Effects of the scaffold on hepatocyte adhesion, viability and function were then investigated. It was showed that hepatocytes on chitosan nanofiber scaffold exhibited better viability and tighter cell-substrate contact than cells on regular chitosan film. In addition, urea synthesis, albumin secretion and cytochrome P450 activity of hepatocytes on chitosan nanofiber scaffold were all 1.5 to 2 folds higher than the controls. Glycogen synthesis was also increased compared with the controls. The new nanofiber scaffold demonstrated superior abilities in cell adhesion and biocompatibility to improved the functions of hepatocytes, which suggested its potential application as a substratum for hepatocytes culturing in bioreactors.