CAJ | 학술논문

目的:初步探讨人乳头瘤病毒16型(HPV16)阳性宫颈癌患者细胞免疫状况,了解其外周血抗原特异性CD8~+细胞毒性T细胞的水平.方法:采取免疫荧光标染重组MHC Ⅰ类分子-肽五聚体技术,运用流式细胞术定量检测患者和正常人外周血CD8~+细胞毒性T细胞、记忆性细胞毒性T细胞、活化细胞毒性T细胞及HPV16抗原特异性CD8~+细胞毒性T细胞.结果:宫颈癌患者CD8~+细胞毒性T细胞计数、记忆性细胞毒性T细胞计数、体内抗原特异性CD8~+细胞毒性T细胞计数和对照组比较差异无统计学意义,但活化细胞毒性T细胞计数低于对照组(P=0.322),体外经抗原肽刺激后抗原特异性CD8~+细胞毒性T细胞计数高于对照组(P=0.0068).结论:HPV16 阳性宫颈癌患者外周血中活化细胞毒性T细胞数目减少,表明患者细胞免疫功能受到一定的影响,通过抗原表位有效的诱导是提高患者的特异性细胞免疫功能的重要途径,运用五聚体技术检测宫颈癌患者抗原特异性细胞毒性T细胞,可初步反映患者特异性细胞免疫状态并有利于进行复发和预后分析.
목적:초보탐토인유두류병독16형(HPV16)양성궁경암환자세포면역상황,료해기외주혈항원특이성CD8~+세포독성T세포적수평.방법:채취면역형광표염중조MHC Ⅰ류분자-태오취체기술,운용류식세포술정량검측환자화정상인외주혈CD8~+세포독성T세포、기억성세포독성T세포、활화세포독성T세포급HPV16항원특이성CD8~+세포독성T세포.결과:궁경암환자CD8~+세포독성T세포계수、기억성세포독성T세포계수、체내항원특이성CD8~+세포독성T세포계수화대조조비교차이무통계학의의,단활화세포독성T세포계수저우대조조(P=0.322),체외경항원태자격후항원특이성CD8~+세포독성T세포계수고우대조조(P=0.0068).결론:HPV16 양성궁경암환자외주혈중활화세포독성T세포수목감소,표명환자세포면역공능수도일정적영향,통과항원표위유효적유도시제고환자적특이성세포면역공능적중요도경,운용오취체기술검측궁경암환자항원특이성세포독성T세포,가초보반영환자특이성세포면역상태병유리우진행복발화예후분석.
Objeotive:The cellular immune response and the antigen-specific CD8~+ cytotoxic T cells (CTLs) in peripheral blood of patients with cervical cancer associated with infection of Human papilloma virus type 16 were investigated. Methods:CD8~+ CTLs, CD8~+ CD45 RO~+ CTLs, and CD8~+ CD69~+ CTLs ( activated CTLs) were detected by flow cytometry, and antigen-specific CD8~+ CTLs were stained with fluorescent-la-beled recombinant MHC class I -peptide pentamer complex. Results;There was no significant difference in the percentage of CD8~+CTLs, CD8~+ CD45RO~+ CTLs, and antigen-specific CD8~+ CTLs between peripheral blood of the patients and that of the control group, however, the percentage of CD8~+ CD69~+ CTLs was lower than that of control group ( P = 0.332 ). Antigen-specific CD8~+ CTLs post-stimulus by peptide in vitro was higher than that of control group ( P = 0.0068 ). Conclusions:The decrease of activated CTLs in peripheral blood of patients with cervical cancer associated with infection by Human papilloma virus type 16 reflects the hampered callular immune function of patients. It is an important pathway to induce antigen-specific CTLs with epitopes for improvement of specific cellular immunity. Detection of antigen-specific CTLs by MHC class I -peptide pentamer complex can probably reflect the specific cellular immunity of patients, and be used for prediction of recurrence and prognosis.