CAJ | 학술논문

背景:目前许多学者对UW液成分进行改进,其目的主要在于进一步探索器官保存的原理,提高保存的技术和延长保存的时限;简化UW液的成分,以进一步满足临床运输、储存和使用的方便;寻找合适的替代品,降低UW液的成本以满足市场需要.目的:观察自制PV液对大鼠肝脏低温保存的效果,并与UW液进行对比.方法:将90只Wistar大鼠按随机数字表法分成3组,即UW液组、PV液组和生理盐水组,制作大鼠肝脏非循环离体灌注模型,分别使用相应灌洗保存液.每组再根据不同的保存时间分为保存0,6,12,18,24 h 5个亚组,每个亚组6只大鼠.测定保存不同时间后肝脏谷丙转氨酶、谷草转氨酶、乳酸脱氢酶活性变化,以及灌注流出液中氧自由基代谢产物丙二醛浓度与超氧化物歧化酶活性;观察胆汁分泌量及镜下肝脏形态学变化.结果与结论:PV液组与UW液组比较,灌注流出液中谷丙转氨酶、谷草转氨酶、乳酸脱氢酶、超氧化物歧化酶活性相近,差异无显著性意义(P>0.05).保存6 h后,PV液组肿瘤坏死因子α质量浓度显著高于UW液组(P<0.05):保存12 h后,UW液组丙二醛浓度显著高于PV液组(P<0.05);保存18 h后,PV液组胆汁分泌量低于UW液组(P<0.05);两组光镜、电镜下组织形态改变相似.提示PV液与UW液对Wistar大鼠肝脏功能均具有保护作用,两者短时间保存效果相似,在抗氧化及清除氧自由基方面,PV液略优于UW液.
배경:목전허다학자대UW액성분진행개진,기목적주요재우진일보탐색기관보존적원리,제고보존적기술화연장보존적시한;간화UW액적성분,이진일보만족림상운수、저존화사용적방편;심조합괄적체대품,강저UW액적성본이만족시장수요.목적:관찰자제PV액대대서간장저온보존적효과,병여UW액진행대비.방법:장90지Wistar대서안수궤수자표법분성3조,즉UW액조、PV액조화생리염수조,제작대서간장비순배리체관주모형,분별사용상응관세보존액.매조재근거불동적보존시간분위보존0,6,12,18,24 h 5개아조,매개아조6지대서.측정보존불동시간후간장곡병전안매、곡초전안매、유산탈경매활성변화,이급관주류출액중양자유기대사산물병이철농도여초양화물기화매활성;관찰담즙분비량급경하간장형태학변화.결과여결론:PV액조여UW액조비교,관주류출액중곡병전안매、곡초전안매、유산탈경매、초양화물기화매활성상근,차이무현저성의의(P>0.05).보존6 h후,PV액조종류배사인자α질량농도현저고우UW액조(P<0.05):보존12 h후,UW액조병이철농도현저고우PV액조(P<0.05);보존18 h후,PV액조담즙분비량저우UW액조(P<0.05);량조광경、전경하조직형태개변상사.제시PV액여UW액대Wistar대서간장공능균구유보호작용,량자단시간보존효과상사,재항양화급청제양자유기방면,PV액략우우UW액.
BACKGROUND: At present, many scholars make improvements to the UW solution composition, aiming to further explore the principle of organ preservation, improve preservation techniques and to extend the preservation time limit; simplified composition of UW solution can further meet the clinical transport, storage and ease of use; suitable alternatives should be found to reduce the cost of UW solution to satisfy the needs of the market.OBJECTIVE: To discuss the effectiveness of self-prepared PV solution for preserving rat's liver at a lower temperature, and to compare the results with UW solution.METHODS: A total of 90 Wistar rats were randomly divided into three groups: UW group, PV group and normal saline (NS) group. Rats in each group were prepared for non-circulated isolated perfused rat liver models, and preserved for 0, 6,12, 18, and 24 hours respectively, with 6 animals in each subgroup. The changes of hepatic enzymology (alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase) in corresponding time period, amount of metabolic production of oxyradicals from perfusion effluent liquid, and the bile secretion and changes in hepatic morphology under the microscope were observed. RESULTS AND CONCLUSION: Amounts of alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase and superoxide dismutase in perfusion effluent liquid were close between the PV group and UW group, and the difference was not significant (P > 0.05). After 6 hours of preservation, tumor necrosis factor a titre in the PV fluid group was greater than that in the UW group (P< 0.05); after 12 hours of preservation, amount of malonaldehyde in the UW group was evidently increased than that in the PV group (P < 0.05); after 18 hours of preservation, bile secretion in the PV group was lower than that in the UW group (P < 0.05); morphological changes of hepatic cells were similar in the two groups under the optical and electron microscope. The results demonstrated that PV solution and UW solution have a similar effect on protecting Wistar rat's liver function, and PV solution is superior to UW solution in antioxidation and scavenging oxygen free radicals.