作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2012年
8期
1407-1415
,共9页
童治军%焦芳婵%吴兴富%王丰青%陈学军%李绪英%高玉龙%张谊寒%肖炳光%吴为人
童治軍%焦芳嬋%吳興富%王豐青%陳學軍%李緒英%高玉龍%張誼寒%肖炳光%吳為人
동치군%초방선%오흥부%왕봉청%진학군%리서영%고옥룡%장의한%초병광%오위인
烤烟%数量性状基因座%遗传作图%加倍单倍体%产量
烤煙%數量性狀基因座%遺傳作圖%加倍單倍體%產量
고연%수량성상기인좌%유전작도%가배단배체%산량
Hue-cured tobacco (Nicotiana tabacum L.)%Quantitative trait locus (QTL)%Genetic mapping%Doubled haploid (DH)%Yield
由于烟草的分子标记开发和遗传图谱构建十分困难,迄今烟草中有关数量性状基因座(QTL)的定位研究仍非常有限.本研究利用一个由207个株系组成的烤烟DH群体及基于该群体所构建的含有24个连锁群、611个SSR标记,总长为1 882.1 cM的遗传图谱,采用复合区间作图方法,对株高(PH)、茎围(SG)、节距(IL)、叶片数(LN)、最大腰叶长(LWL)和最大腰叶宽(WWL)6个与叶片产量有关的农艺性状进行QTL定位分析.共检测到69个QTL,大部分QTL的效应值较小,仅有4个具有较大的效应值,可解释大约15％～20％的表型变异.6个性状之间大多彼此相关.与此相符,在基因组中发现存在许多小区域,每个区域包含2个或2个以上紧密连锁的不同性状的QTL.
由于煙草的分子標記開髮和遺傳圖譜構建十分睏難,迄今煙草中有關數量性狀基因座(QTL)的定位研究仍非常有限.本研究利用一箇由207箇株繫組成的烤煙DH群體及基于該群體所構建的含有24箇連鎖群、611箇SSR標記,總長為1 882.1 cM的遺傳圖譜,採用複閤區間作圖方法,對株高(PH)、莖圍(SG)、節距(IL)、葉片數(LN)、最大腰葉長(LWL)和最大腰葉寬(WWL)6箇與葉片產量有關的農藝性狀進行QTL定位分析.共檢測到69箇QTL,大部分QTL的效應值較小,僅有4箇具有較大的效應值,可解釋大約15％～20％的錶型變異.6箇性狀之間大多彼此相關.與此相符,在基因組中髮現存在許多小區域,每箇區域包含2箇或2箇以上緊密連鎖的不同性狀的QTL.
유우연초적분자표기개발화유전도보구건십분곤난,흘금연초중유관수량성상기인좌(QTL)적정위연구잉비상유한.본연구이용일개유207개주계조성적고연DH군체급기우해군체소구건적함유24개련쇄군、611개SSR표기,총장위1 882.1 cM적유전도보,채용복합구간작도방법,대주고(PH)、경위(SG)、절거(IL)、협편수(LN)、최대요협장(LWL)화최대요협관(WWL)6개여협편산량유관적농예성상진행QTL정위분석.공검측도69개QTL,대부분QTL적효응치교소,부유4개구유교대적효응치,가해석대약15％～20％적표형변이.6개성상지간대다피차상관.여차상부,재기인조중발현존재허다소구역,매개구역포함2개혹2개이상긴밀련쇄적불동성상적QTL.
To identify quantitative trait lici (QTLs) for important traits in tobacco,a doubled haploid (DH) population derived form the cross between flue-cured tobacco cultivars Honghua Dajinyuan and Hicks Broad Leaf was used in phenotypic survey and SSR analysis.Six leaf yield-related traits,i.e.,plant height (PH),stem girth (SG),internode length (IL),leaf number (LN),length of the largest waist leaf (LWL),and width of the largest waist leaf (WWL),were tested,which showed large correlations between each other.QTL mapping was carried out using the composite interval mapping method based a genetic map covering 1882.1 cM of tobacco genome with 611 SSR markers in 24 linkage groups.A total of 69 QTLs were detected,most of which exhibited small additive effects.Four QTLs associated with PH,IL,and WWL exhibited much higher phenotypic contributions than other QTLs,and a single locus explained 15-20％ of the phenotypic variation.Many small regions were detected to harbor two or more closely linked QTLs for different traits.This result was in agreement with the correlation analysis of phenotypic traits.
