CAJ | 학술논문

目的比较背部皮下与肾包囊内2种不同移植方式所建立的类风湿关节炎(RA)滑膜侵蚀软骨联合免疫缺陷(SCID)模型的病理学及血清学改变,为采用人滑膜和软骨进行RA炎症及软骨损伤机制和治疗的体内研究提供实验系统.方法 15只SCID小鼠随机分为皮下移植组、肾包囊移植组及空白对照组,无菌获取RA患者滑膜及人正常软骨,通过相应的移植方式共同植入各组SCID小鼠体内.分别于术后4、8周取移植组织,进行组织病理学分析;留取血清,用酶联免疫吸附试验(ELISA)检测人类风湿因子(RF)水平.结果皮下移植组成活率及建模成功率较肾包囊组明显增高,两组滑膜中炎细胞浸润、滑膜增殖、软骨侵蚀评分及血清RF-IgM水平差异均无统计学意义(P>0.05).结论与肾包囊移植相比,背部皮下移植建立的SCID-HuRAg在炎症及骨侵蚀及血清学改变上差异无统计学意义,且操作简便,成功率高,是进行人RA滑膜对软骨侵蚀机制和治疗体内研究的理想动物模型.
목적 비교배부피하여신포낭내2충불동이식방식소건립적류풍습관절염(RA)활막침식연골연합면역결함(SCID)모형적병이학급혈청학개변,위채용인활막화연골진행RA염증급연골손상궤제화치료적체내연구제공실험계통.방법 15지SCID소서수궤분위피하이식조、신포낭이식조급공백대조조,무균획취RA환자활막급인정상연골,통과상응적이식방식공동식입각조SCID소서체내.분별우술후4、8주취이식조직,진행조직병이학분석;류취혈청,용매련면역흡부시험(ELISA)검측인류풍습인자(RF)수평.결과 피하이식조성활솔급건모성공솔교신포낭조명현증고,량조활막중염세포침윤、활막증식、연골침식평분급혈청RF-IgM수평차이균무통계학의의(P>0.05).결론 여신포낭이식상비,배부피하이식건립적SCID-HuRAg재염증급골침식급혈청학개변상차이무통계학의의,차조작간편,성공솔고,시진행인RA활막대연골침식궤제화치료체내연구적이상동물모형.
Objective To compare the pathological and serological difference of rheumatoid arthritis (RA) models in severe combined immunodeficiency (SCID) mice transplanted with synovial tissues from patients with rheumatoid arthritis (SCID-HuRAg mice) established either by renal capsule or subcutaneous back heterotopic transplantation. Methods RA synovium and normal human cartilage were co-implanted subcutaneously into the backs or under the renal capsule of 15 SCID mice. Engrafted tissues and serum were taken at the 4th and 8th week after transplantation. Histopathology and ELISA were performed to compare their histological and serological differences with RA. Results The morbidity and taken rate were significantly increased in the subcutaneous back of the mice group than the renal capsule group. The degree of cartilage erosion as well as the titers of serum IgM type rheumatoid factor suggested no significant difference between the two groups of SCID-HuRAg model devel oped by different engraft methods. Conclusion Back subcutaneous transplantation SCID-HuRAg model can be an ideal and stable animal model for studies on the pathogenesis and biotherapy of RA.