中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2009年
6期
332-335
,共4页
蔡扬%柳咏发%杨宏%卢虹
蔡颺%柳詠髮%楊宏%盧虹
채양%류영발%양굉%로홍
癌,鳞状细胞%中心体%流式细胞术%p21waf1
癌,鱗狀細胞%中心體%流式細胞術%p21waf1
암,린상세포%중심체%류식세포술%p21waf1
Carcinoma,squamous cell%Centrosome%Flow cytometry%p21waf1
目的 了解口腔鳞状细胞癌(oral squamous cell cancer,OSCC)p21waf1蛋白表达与中心体扩增的相关性,探讨p53-p21waf1通路在OSCC中心体扩增中的作用及意义.方法 8例正常口腔黏膜及27例OSCC石蜡包埋组织,采用间接免疫荧光双重染色法了解OSCC组织中心体扩增情况;采用流式细胞术及免疫组织化学方法对相应组织p21waf1蛋白表达量及突变型p53蛋白进行检测,分析三者间的相关性.结果 中心体数目扩增(＞2个/细胞)可见于78%(21/27)的OSCC组织,p21waf1蛋白表达量在有中心体扩增的OSCC组织中[(0.878±0.081)]低于无中心体扩增的OSCC组织[(0.952±0.018),t=3.838,P=0.001],OSCC组织中心体扩增程度与p21waf1蛋白表达量间存在负相关关系(r=0.472,P＜0.05);p21waf1蛋白表达量在OSCC组织p53阳性组[(0.823±0.071)]低于p53阴性组[(0.909±0.075),t=3.905,P＜0.01],两者间存在负相关关系(r=-0.491,P＜0.05).结论 p53-p21waf1通路可能参与了OSCC中心体循环调控,p53突变导致的p21waf1蛋白表达下调在OSCC中心体扩增中可能起一定作用.
目的 瞭解口腔鱗狀細胞癌(oral squamous cell cancer,OSCC)p21waf1蛋白錶達與中心體擴增的相關性,探討p53-p21waf1通路在OSCC中心體擴增中的作用及意義.方法 8例正常口腔黏膜及27例OSCC石蠟包埋組織,採用間接免疫熒光雙重染色法瞭解OSCC組織中心體擴增情況;採用流式細胞術及免疫組織化學方法對相應組織p21waf1蛋白錶達量及突變型p53蛋白進行檢測,分析三者間的相關性.結果 中心體數目擴增(＞2箇/細胞)可見于78%(21/27)的OSCC組織,p21waf1蛋白錶達量在有中心體擴增的OSCC組織中[(0.878±0.081)]低于無中心體擴增的OSCC組織[(0.952±0.018),t=3.838,P=0.001],OSCC組織中心體擴增程度與p21waf1蛋白錶達量間存在負相關關繫(r=0.472,P＜0.05);p21waf1蛋白錶達量在OSCC組織p53暘性組[(0.823±0.071)]低于p53陰性組[(0.909±0.075),t=3.905,P＜0.01],兩者間存在負相關關繫(r=-0.491,P＜0.05).結論 p53-p21waf1通路可能參與瞭OSCC中心體循環調控,p53突變導緻的p21waf1蛋白錶達下調在OSCC中心體擴增中可能起一定作用.
목적 료해구강린상세포암(oral squamous cell cancer,OSCC)p21waf1단백표체여중심체확증적상관성,탐토p53-p21waf1통로재OSCC중심체확증중적작용급의의.방법 8례정상구강점막급27례OSCC석사포매조직,채용간접면역형광쌍중염색법료해OSCC조직중심체확증정황;채용류식세포술급면역조직화학방법대상응조직p21waf1단백표체량급돌변형p53단백진행검측,분석삼자간적상관성.결과 중심체수목확증(＞2개/세포)가견우78%(21/27)적OSCC조직,p21waf1단백표체량재유중심체확증적OSCC조직중[(0.878±0.081)]저우무중심체확증적OSCC조직[(0.952±0.018),t=3.838,P=0.001],OSCC조직중심체확증정도여p21waf1단백표체량간존재부상관관계(r=0.472,P＜0.05);p21waf1단백표체량재OSCC조직p53양성조[(0.823±0.071)]저우p53음성조[(0.909±0.075),t=3.905,P＜0.01],량자간존재부상관관계(r=-0.491,P＜0.05).결론 p53-p21waf1통로가능삼여료OSCC중심체순배조공,p53돌변도치적p21waf1단백표체하조재OSCC중심체확증중가능기일정작용.
Objective To elucidate the possible role of p53-p21waf1 pathway for centrosome amplification in oral squamous cell carcinoma ( OSCC ) . Methods Formalin-fixed, paraffin-embedded tissues of 8 cases of normal oral epithelium tissues and 27 cases of OSCC tissues were examined for the expression of p21waf1 and mutated p53 proteins by flowcytometry and immunohistochemistry, and centrosome status was investigated by indirect immunofluorescence double staining with antibodies to centrosome protein gamma-tubulin and cytokeratin. The correlation between p21waf1, p53 and centrosome amplification in OSCC was statistically analyzed by SPSS 12.0. Results All normal oral epithelium tissues showed normal centrosomes( 1-2 centrosomes per cell) in epithelium cells, while 21 out of 27 cases(78%) of OSCC showed the evidence of centrosome amplification characterized by supernumerary centrosomes (＞2 centrosomes per cell) in a fraction of tumor cells. The quantity of p21waf1 protein was lower in OSCC with centrosome amplification [(0. 878±0. 081 )] than that in OSCC without centrosome amplification [ (0. 952±0. 018 ) ,t =3.838,P<0.01] , and negative correlations were found between the quantity of p21waf1 protein and the degree of centrosome amplification ( r = - 0.472, P＜0. 05) , as well as the positive staining of p53 ( r =-0.491 ,P＜0. 01). Conclusions p53-p21waf1 pathway might involve in centrosome duplication cycle in OSCC. Down-regulated p21waf1 protein, via p53 transactivation-dependent mechanism, was likely a contributing factor towards centrosome amplification in OSCC.
