中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
8期
1443-1446
,共4页
黄陆力%王崇军%付庆林%韩培立%张新中%刘永强%王丽娜%崔勤涛%周朝元
黃陸力%王崇軍%付慶林%韓培立%張新中%劉永彊%王麗娜%崔勤濤%週朝元
황륙력%왕숭군%부경림%한배립%장신중%류영강%왕려나%최근도%주조원
心肌梗死%银杏叶提取物%心肌保护%黏附分子%白细胞介素-6
心肌梗死%銀杏葉提取物%心肌保護%黏附分子%白細胞介素-6
심기경사%은행협제취물%심기보호%점부분자%백세포개소-6
Myocardial infarction%Ginkgo biloba extract%Myocardial protection%Adhesion molecule%Interleukin-6
目的 观察大鼠急性心肌梗死时心肌中细胞间黏附分子-1 (ICAM-1)和白细胞介素(IL)-6的表达及银杏叶提取物对上述指怀的影响,探讨银杏叶提取物对大鼠急性心肌梗死损伤心肌的保护作用.方法 选择健康雌性SD大鼠109只,结扎左冠状动脉前降的方法制备急性心肌梗死模型,随机分为心肌梗死模型组(A组,n=38)、银杏叶治疗组(R组,n=39)和假手术组(C组,n=32).A组结扎左冠状动脉前降支；B组术前30 min尾静脉注射银杏叶提取物2 mg/kg；C组仅在前降支留置一松结,不结扎各组再按术后观察时点(2h、6h、48 h、7d)随机分为4个亚组,分别于各观察时点采集各亚组大鼠心脏标本,苏木素-伊红(HE)染色光镜下观察形态学变化,免疫组织化学法检测ICAM-1和IL-6的表达.结果 (1)HE染色显示A组大鼠左心室前壁心外膜下心肌细胞数量减少,心肌间质充血、水肿、心肌纤维溶解、片状坏死,伴有多量炎性细胞浸润,坏死心肌范围超过心壁厚度的1/2以上,心内膜侧仍见大量存活心肌细胞；B组心肌损伤程度较A组减轻；C组大鼠左心室前壁心外膜下散在心肌间质充血、水肿,未见心肌细胞坏死.(2)免疫组织化学结果显示,在术后2h、6h、48 h、7d各时间点A组ICAM-1( 493.02±86.32、550.05±87.36、695.05±117.35、807.56±132.05)和IL-6(153.02±26.32、179.05±28.36、185.35±37.05、161.18±31.05)、B组ICAM-1(434.83±75.63、508.45±96.21、596.28±105.36、679.25±129.54)和IL-6蛋白含量(137.83±33.63、155.42±26.21、157.28±25.36、135.25±26.54)均高于C组的ICAM-1 (310.59±82.31、330.27±80.32、320.95±81.36、341.84±82.35)和IL-6( 87.59±22.31、97.27±26.32、93.95±19.36、91.84 ±24.35),差异有统计学意义(P＜0.05)；A组各观察时点ICAM-1和IL-6蛋白含量均高于B组,差异有统计学意义(P＜0.05).结论 (1)大鼠急性心肌梗死模型心肌中ICAM-1和IL-6的表达上调,上述指标可反映心肌损伤的程度和冠脉病变范围.(2)大鼠急性心肌梗死中应用银杏叶提取物干预,可下调心肌中ICAM-1和IL-6的表达,减轻大鼠心肌损伤程度,证实银杏叶提取物有较好的心肌保护作用.
目的 觀察大鼠急性心肌梗死時心肌中細胞間黏附分子-1 (ICAM-1)和白細胞介素(IL)-6的錶達及銀杏葉提取物對上述指懷的影響,探討銀杏葉提取物對大鼠急性心肌梗死損傷心肌的保護作用.方法 選擇健康雌性SD大鼠109隻,結扎左冠狀動脈前降的方法製備急性心肌梗死模型,隨機分為心肌梗死模型組(A組,n=38)、銀杏葉治療組(R組,n=39)和假手術組(C組,n=32).A組結扎左冠狀動脈前降支；B組術前30 min尾靜脈註射銀杏葉提取物2 mg/kg；C組僅在前降支留置一鬆結,不結扎各組再按術後觀察時點(2h、6h、48 h、7d)隨機分為4箇亞組,分彆于各觀察時點採集各亞組大鼠心髒標本,囌木素-伊紅(HE)染色光鏡下觀察形態學變化,免疫組織化學法檢測ICAM-1和IL-6的錶達.結果 (1)HE染色顯示A組大鼠左心室前壁心外膜下心肌細胞數量減少,心肌間質充血、水腫、心肌纖維溶解、片狀壞死,伴有多量炎性細胞浸潤,壞死心肌範圍超過心壁厚度的1/2以上,心內膜側仍見大量存活心肌細胞；B組心肌損傷程度較A組減輕；C組大鼠左心室前壁心外膜下散在心肌間質充血、水腫,未見心肌細胞壞死.(2)免疫組織化學結果顯示,在術後2h、6h、48 h、7d各時間點A組ICAM-1( 493.02±86.32、550.05±87.36、695.05±117.35、807.56±132.05)和IL-6(153.02±26.32、179.05±28.36、185.35±37.05、161.18±31.05)、B組ICAM-1(434.83±75.63、508.45±96.21、596.28±105.36、679.25±129.54)和IL-6蛋白含量(137.83±33.63、155.42±26.21、157.28±25.36、135.25±26.54)均高于C組的ICAM-1 (310.59±82.31、330.27±80.32、320.95±81.36、341.84±82.35)和IL-6( 87.59±22.31、97.27±26.32、93.95±19.36、91.84 ±24.35),差異有統計學意義(P＜0.05)；A組各觀察時點ICAM-1和IL-6蛋白含量均高于B組,差異有統計學意義(P＜0.05).結論 (1)大鼠急性心肌梗死模型心肌中ICAM-1和IL-6的錶達上調,上述指標可反映心肌損傷的程度和冠脈病變範圍.(2)大鼠急性心肌梗死中應用銀杏葉提取物榦預,可下調心肌中ICAM-1和IL-6的錶達,減輕大鼠心肌損傷程度,證實銀杏葉提取物有較好的心肌保護作用.
목적 관찰대서급성심기경사시심기중세포간점부분자-1 (ICAM-1)화백세포개소(IL)-6적표체급은행협제취물대상술지부적영향,탐토은행협제취물대대서급성심기경사손상심기적보호작용.방법 선택건강자성SD대서109지,결찰좌관상동맥전강적방법제비급성심기경사모형,수궤분위심기경사모형조(A조,n=38)、은행협치료조(R조,n=39)화가수술조(C조,n=32).A조결찰좌관상동맥전강지；B조술전30 min미정맥주사은행협제취물2 mg/kg；C조부재전강지류치일송결,불결찰각조재안술후관찰시점(2h、6h、48 h、7d)수궤분위4개아조,분별우각관찰시점채집각아조대서심장표본,소목소-이홍(HE)염색광경하관찰형태학변화,면역조직화학법검측ICAM-1화IL-6적표체.결과 (1)HE염색현시A조대서좌심실전벽심외막하심기세포수량감소,심기간질충혈、수종、심기섬유용해、편상배사,반유다량염성세포침윤,배사심기범위초과심벽후도적1/2이상,심내막측잉견대량존활심기세포；B조심기손상정도교A조감경；C조대서좌심실전벽심외막하산재심기간질충혈、수종,미견심기세포배사.(2)면역조직화학결과현시,재술후2h、6h、48 h、7d각시간점A조ICAM-1( 493.02±86.32、550.05±87.36、695.05±117.35、807.56±132.05)화IL-6(153.02±26.32、179.05±28.36、185.35±37.05、161.18±31.05)、B조ICAM-1(434.83±75.63、508.45±96.21、596.28±105.36、679.25±129.54)화IL-6단백함량(137.83±33.63、155.42±26.21、157.28±25.36、135.25±26.54)균고우C조적ICAM-1 (310.59±82.31、330.27±80.32、320.95±81.36、341.84±82.35)화IL-6( 87.59±22.31、97.27±26.32、93.95±19.36、91.84 ±24.35),차이유통계학의의(P＜0.05)；A조각관찰시점ICAM-1화IL-6단백함량균고우B조,차이유통계학의의(P＜0.05).결론 (1)대서급성심기경사모형심기중ICAM-1화IL-6적표체상조,상술지표가반영심기손상적정도화관맥병변범위.(2)대서급성심기경사중응용은행협제취물간예,가하조심기중ICAM-1화IL-6적표체,감경대서심기손상정도,증실은행협제취물유교호적심기보호작용.
Objective To study the expressiou of intercellular adhesion molecule-1 (ICAM-1) and interleukin (IL)-6 in myocardium with acute myocardial infarction in rats and the influence of ginkgo biloba extract on them,and to explore the mechanism of protective effects of ginkgo biloba extract on acute infarcted myocardium.Methods One hundred and nine healthy female SD rats were selected to make acute myocardial infartion model by ligation of the left anterior descending (LAD) coronary artery,and then they were randomly divided into model group ( group A,n =38 ),ginkgo biloba treatment group ( group B,n =39 ),and sham operation group ( group C,n =32).In goup A,the left anterior descending (LAD) coronary artery was ligated solely; in group B,Ginkgo biloba extract (2 mg/kg body weight) was intravenously injected 30 min before ligation of LAD coronary artery; in group C,a loose knol was made around the first descending branch of LAD coronary arterv,witbout ligation.Each group was then randomly divided into 4 subgroups,which were observed 2 h,6 h,48 h,and 7 days after operation,respectively.Blood and heart samples were collected at every time point.Morphological changes were observed by HE staining method under the light microscopy,and ICAM-1 and IL-6 expression was detected by using immunohistochemistry.Results By using HE staining,in group A,the number of myocardial cells was decreased,and myocardial interstitial congestion,edema,myocardial fiber dissolution,flaky necrosis and infiltration of inflammatory cells were observed.Range of myocardial infarction was more than 1/2 of cardiac wall,and large number of viable myocardial cells still could be seen in the other side of cardiac wall; In group B,myocardial injuries were significantly milder than those in group A ; In group C,there were myocardial interstitial scattered congestion,edema in subepicardium of left ventricular anterior wall,but without myocardial necrosis.Immunohistochemistry revealed that at 2nd h,6th h,48th h,and 7th day,the protein levels of ICAM-1 (493.02 ± 86.32,550.05 ±87.36,695.05 ± 117.35,807.56 ± 132.05 ) and IL-6 ( 153.02 ± 26.32,179.05 ± 28.36,185.35 ±37.05,161.18 ±31.05) in group A,and those [(ICAM-1:434.83 ±75.63,508.45 ±96.21,596.28 ±105.36,679.25 ± 129.54 ) and ( IL-6:137.83 ± 33.63,155.42 ± 26.21,157.28 ± 25.36,135.25 ±26.54)] in group B were significantly higher than those in group C ( ICAM-1:310.59 ± 82.31,330.27 ±80.32,320.95 ± 81.36,341.84 ± 82.35 ; IL-6:87.59 ± 22.31,97.27 26.32,93.95 ± 19.36,91.84 ±24.35),respectively (P＜0 05).ICAM-1 and IL-6 protein levels in group A were significantly higher than those in group B at each time point,respectively ( P ＜ 0.05).Conclusion ( 1 ) The expression of ICAM-1 and IL-6 was upregulated in acute infracted myocardium; (2) The application of ginkgo biloba extract can downregulate the expression of ICAM-1 and IL-6,and reduce the extent of myocardial injury,suggesting the protective effect of gnkgo biloba extract on ischemic myocardium.
