CAJ | 학술논문

目的模拟急性胆源性胰腺炎的发病条件,制作一种符合临床特点的大鼠急性坏死性胰腺炎模型.方法设计大鼠胰胆管单扎和双扎两种结扎方法,测压并用2%甘氨脱氧胆酸(GDCA)以35 cm H2O(1 cm H2O=0.098 kPa)压力恒压灌注5 min,8、16、24 h剖杀,观察急性胰腺炎的发生情况.结果单扎组胆胰管压力为:(20.60±1.51)cm H2O,双扎组为:(29.37±0.87)cmH20.两组均诱导急性胰腺炎,于24 h表现出坏死性胰腺炎特征.病理学评分显示,随时间进程,胰腺炎逐渐加重(P＜0.01).在各时间点,胆胰管双扎组炎症重于单扎组(P＜0.01).结论恒压灌注2%GDCA联合胰胆管单扎和双扎均可诱导出大鼠急性坏死性胰腺炎;胰管压力及其持续时间与急性胰腺炎发生、发展密切相关.
목적 모의급성담원성이선염적발병조건,제작일충부합림상특점적대서급성배사성이선염모형.방법 설계대서이담관단찰화쌍찰량충결찰방법,측압병용2%감안탈양담산(GDCA)이35 cm H2O(1 cm H2O=0.098 kPa)압력항압관주5 min,8、16、24 h부살,관찰급성이선염적발생정황.결과 단찰조담이관압력위:(20.60±1.51)cm H2O,쌍찰조위:(29.37±0.87)cmH20.량조균유도급성이선염,우24 h표현출배사성이선염특정.병이학평분현시,수시간진정,이선염축점가중(P＜0.01).재각시간점,담이관쌍찰조염증중우단찰조(P＜0.01).결론 항압관주2%GDCA연합이담관단찰화쌍찰균가유도출대서급성배사성이선염;이관압력급기지속시간여급성이선염발생、발전밀절상관.
Objective To establish a new acute necrotizing pancreatitis model in rats. Methods We designed single pancreatic bile duct ligation and double pancreatic bile duct ligation in rats, combined with retrogradely infusing 2% glycodeoxycholic acid ( GDCA), to test duct pressure and observe the severity of acute pancreatitis at 8, 16 and 24 h, respectively. Results In two groups, the duct pressures were (20. 60 ± 1.51 ) cm H2O and (29. 37 ±0. 87) cm H2O, respectively. Both of the two methods could induce typical acute necrotizing pancreatitis at 24 h, the severity of which progressively developed with time course(P ＜0. 01 ), at each time point, the severity of double duct ligation group was higher than single duct ligation group ( P ＜ 0. 01 ). Conclusion Retrogradely infusing 2% GDCA with constant pressure,combined with the two methods of pancreatic bile duct ligation, coude induce clinically relevant acute necrotizing pancreatitis in rats; the pancreatic duct pressure and its duration might be closely related to the arising and developing of acute pancreatitis.