CAJ | 학술논문

目的：研究扶正祛邪丹对K562细胞株和小鼠造血干细胞的影响，探讨扶正祛邪丹治疗骨髓增生异常综合征的疗效机理。方法：扶正祛邪丹含药兔血清培养K562细胞株，用流式细胞仪检测P53蛋白表达、细胞活力、细胞凋亡与细胞周期；用造血干细胞培养法观察扶正祛邪丹对BALB/C小鼠骨髓造血干细胞增殖的影响。结果：扶正祛邪丹对K562细胞株P53蛋白表达有抑制作用，对红系造血祖细胞有促进作用。不能诱导细胞凋亡，对细胞增殖周期无明显影响，对小鼠骨髓粒系造血干细胞无明显促进作用。结论：扶正祛邪丹促进红系造血干细胞增殖，降低P53蛋白表达，可能为其主要疗效机理。
목적：연구부정거사단대K562세포주화소서조혈간세포적영향，탐토부정거사단치료골수증생이상종합정적료효궤리。방법：부정거사단함약토혈청배양K562세포주，용류식세포의검측P53단백표체、세포활력、세포조망여세포주기；용조혈간세포배양법관찰부정거사단대BALB/C소서골수조혈간세포증식적영향。결과：부정거사단대K562세포주P53단백표체유억제작용，대홍계조혈조세포유촉진작용。불능유도세포조망，대세포증식주기무명현영향，대소서골수립계조혈간세포무명현촉진작용。결론：부정거사단촉진홍계조혈간세포증식，강저P53단백표체，가능위기주요료효궤리。
Objective:To study the mechanism of Fuzheng Quxie Tablet (FZQXD) in treating myelodysplastic syndromes (MDS).Methods: The in vitro effect of FZQXD on K562 cell line and hematopietic stem cells were studied with flow cytometery and cell cultuer.Results:FZQXD inhibited P53 protein expression,markedly promoted the proliferation of colony forming unit-erythrocytes(CFU-E).no effect on K562 cell in inducing apoptosis and cell-cycle.Conclusions:That FZQXD slightly inhibited P53 protein expression and markedly promoted the proliferation of colony forming unit-erythrocytes (CFU-E) might be one of the mechanisms in treating myelodysplastic syndromes.