CAJ | 학술논문

利用层层自组装方法制备了聚烯丙基铵盐酸盐(PAH)/聚苯乙烯磺酸钠(PSS)多层膜. 通过吸附或共价偶联, 在多层膜表面修饰了聚乙二醇(PEG)、牛血清白蛋白(BSA)或肝素, 通过石英晶体微天平(QCM)、椭圆偏振光谱和原子力显微镜(AFM)研究了多层膜的表面形貌及修饰方法对各种蛋白的吸附性能. 经修饰后的多层膜较基底膜的厚度均有所增大;最外层经修饰后的多层膜吸附的BSA、纤维蛋白原及血浆蛋白的量较未修饰多层膜均有所减少. 采用SEM观察了血小板在多层膜上的黏附情况和形态变化, 计算了血小板的黏附率. 比较各多层膜的凝血酶原时间(PT), 发现修饰后的多层膜的凝血酶原时间均有所延长, 但各组间无显著性差异.
이용층층자조장방법제비료취희병기안염산염(PAH)/취분을희광산납(PSS)다층막. 통과흡부혹공개우련, 재다층막표면수식료취을이순(PEG)、 우혈청백단백(BSA)혹간소, 통과석영정체미천평(QCM)、타원편진광보화원자력현미경(AFM)연구료다층막적표면형모급수식방법대각충단백적흡부성능. 경수식후적다층막교기저막적후도균유소증대;최외층경수식후적다층막흡부적BSA、섬유단백원급혈장단백적량교미수식다층막균유소감소. 채용SEM관찰료혈소판재다층막상적점부정황화형태변화, 계산료혈소판적점부솔. 비교각다층막적응혈매원시간(PT), 발현수식후적다층막적응혈매원시간균유소연장, 단각조간무현저성차이.
Poly ( styrene sulfonate )/poly (allylamine hydrochloride) multilayers were assembled on silicone wafers via the technique of layer-by-layer assembly.Their surfaces were further modified either physically or chemically with bovine serum albumin(BSA) , heparin and polyethylene glycol(PEG). Protein adsorption on these surfaces was investigated by quartz crystal microbalance (QCM),ellipsometry and atomic force microscopy(AFM). The dynamic adsorption process of BSA on these multilayers was monitored by QCM, revealing that the adsorption equilibrium was rapidly achieved within 3 min on the control and heparin adsorbed multilayer surfaces, and within 5-10 min on the chemically bonded BSA and PEG surfaces, but more than 80 min on the BSA physically modified surface. After adsorption of BSA or fibrinogen, all the modified multilayers became smoother due to the effect of " surface valley adsorption". Ellipsometry characterization found that the adsorbed mount of BSA, fibrinogen and plasma proteins on all the modified multilayers were smaller than that of the unmodified control multilayers. The platelet adsorption on the multilayers was analyzed by SEM, revealing that the number of the adsorbed platelets on all the modified surfaces except of the heparin modified one was significantly reduced. The prothrombin time(PT) of all the modified multilayers was prolonged compared with that of the unmodified multilayers, but there was no significant difference between all the samples.