CAJ | 학술논문

背景与目的:探讨在前列腺穿刺活检标本组织中联合检测α-甲酰基辅酶A消旋酶(P504S)、P63蛋白和高分子量角蛋白(34βE12)对前列腺良恶性病变诊断及鉴别诊断的意义.材料与方法:采用组合式单克隆抗体和双酶标记的免疫组化鸡尾酒法检测32例前列腺癌(PCa)、16例前列腺不典型腺瘤样增生(AAH)和44例良性前列腺增生(BPH)穿刺活检标本,在同一张组织切片上检测P504S、P63和34βE12抗原的表达情况. 结果:PCa中P504S均呈阳性表达,阳性率100%,而P63和34βE12呈阴性表达;AAH中上述3种蛋白的阳性表达率均为75%;BPH中P504S均呈阴性表达,而P63、34βE12均呈阳性表达,阳性率100%;P504s、P63和34βE12抗原在PCa、AAH、BPH 3组标本中的表达两两比较差异均有统计学意义(P<0.05).结论:免疫组化鸡尾酒法可作为前列腺疾病诊断及鉴别诊断的一种有效辅助手段;P504S是PCa高度敏感和特异的标志物.P63和34βE12联合标记基底细胞的特异性高,3者联合检测能提高前列腺穿刺活检标本诊断的准确性.
배경여목적:탐토재전렬선천자활검표본조직중연합검측α-갑선기보매A소선매(P504S)、P63단백화고분자량각단백(34βE12)대전렬선량악성병변진단급감별진단적의의.재료여방법:채용조합식단극륭항체화쌍매표기적면역조화계미주법검측32례전렬선암(PCa)、16례전렬선불전형선류양증생(AAH)화44례량성전렬선증생(BPH)천자활검표본,재동일장조직절편상검측P504S、P63화34βE12항원적표체정황. 결과:PCa중P504S균정양성표체,양성솔100%,이P63화34βE12정음성표체;AAH중상술3충단백적양성표체솔균위75%;BPH중P504S균정음성표체,이P63、34βE12균정양성표체,양성솔100%;P504s、P63화34βE12항원재PCa、AAH、BPH 3조표본중적표체량량비교차이균유통계학의의(P<0.05).결론:면역조화계미주법가작위전렬선질병진단급감별진단적일충유효보조수단;P504S시PCa고도민감화특이적표지물.P63화34βE12연합표기기저세포적특이성고,3자연합검측능제고전렬선천자활검표본진단적준학성.
BACKGROUND AND AIM: To examine the significance of P504S, p63, 34βE12 cocktail in the differential diagnosis of prostate lesion in needle biopsy specimen. MATERIALS AND METHODS: The expressions of P504S, P63 and 34βE12 were examined in the biopsy specimens of 32 prostate cancer(PCa), 16 prostatic atypical adenomatous hyperplasia (AAH)and 44 benign prostatic hyperplasia (BPH) using immunohistochemical method with triple-antibody cocktail (P504S, P63, 34βE12) staining and double-color chromogens in single paraffin sections. RESULTS: PCa stained positive for P504S and negative for P63 and 34βE12, and the positivity rate of P504S was 100% . The positive rates in AAH were all 75% for the three proteins. BPH strongly expressed P63 and 34βE12, but not P540S. The expressions of P504S, P63 and 340E12 were significantly different among PCa, AAH and BPH (P<0.05) . CONCLUSION: Cocktail staining may be an effective approach in the diagnosis and differential diagnosis of prostate lesion.P504S was a sensitive and specific marker for PCa. P63 and 34βE12 cocktail staining could increase the sensitivity and specificity for the basal cell layer. The triple-antibody cocktail staining could improve diagnostic accuracy of prostate lesion in needle biopsy specimen, and important for the early diagnosis of prostate cancer.