CAJ | 학술논문

目的:探讨结缔组织生长因子(connective tissue growth factor,CTGF)对大鼠正畸牙移动模型牙周组织改建的影响.方法:雄性SD大鼠45 只,牵引下颌第一磨牙近中移动制作大鼠牙移动模型.模型完成后,随机分为3 个实验组: CTGF组,生理盐水对照组及加力对照组,每组15 只.从0 d开始分别将CTGF及生理盐水每隔1 d注射到CTGF组及生理盐水对照组左侧下第一磨牙舌侧骨黏膜下,分别于干预后1、3、7、15、21 d每组各取3 只大鼠的牙周组织,制备组织切片,HE及免疫组化染色,计算机图像分析检测牙周组织中VEGF的表达状况.结果:正畸加力后,大鼠牙周组织中伴随牙周组织改建,牙周组织中VEGF表达增高.加力7 d牙周组织改建最为活跃,VEGF表达达到高峰期.CTGF干预治疗后,CTGF组加力后7、15 d VEGF表达强度增幅最高,CTGF组与对照组间差异有显著性.结论: CTGF干预可上调正畸大鼠牙周组织中VEGF表达,加速正畸牙周组织改建.
목적:탐토결체조직생장인자(connective tissue growth factor,CTGF)대대서정기아이동모형아주조직개건적영향.방법:웅성SD대서45 지,견인하합제일마아근중이동제작대서아이동모형.모형완성후,수궤분위3 개실험조: CTGF조,생리염수대조조급가력대조조,매조15 지.종0 d개시분별장CTGF급생리염수매격1 d주사도CTGF조급생리염수대조조좌측하제일마아설측골점막하,분별우간예후1、3、7、15、21 d매조각취3 지대서적아주조직,제비조직절편,HE급면역조화염색,계산궤도상분석검측아주조직중VEGF적표체상황.결과:정기가력후,대서아주조직중반수아주조직개건,아주조직중VEGF표체증고.가력7 d아주조직개건최위활약,VEGF표체체도고봉기.CTGF간예치료후,CTGF조가력후7、15 d VEGF표체강도증폭최고,CTGF조여대조조간차이유현저성.결론: CTGF간예가상조정기대서아주조직중VEGF표체,가속정기아주조직개건.
Objective: To study the effects of CTGF on the periodontal tissue remolding during orthodontic tooth movement. Methods: A mouse model of orthodontic tooth movement was established. The CTGF and NS were injected into the periodontal tissue of the first molar. HE staining, immunohistochemical staining and automatic image analysis system were used to detect the changes of periodontal tissue and the expression of VEGF. Results: The periodontal tissue remolding accompanied the over expression of VEGF during the orthodontic tooth movement. At day 7, the remolding was most active and the expression of VEGF was the highest. CTGF injection induced acceleration of periodontal remolding and up-regulating the expression of VEGF during orthodontic tooth movement, especially at day 7 and day 15 after the orthodontic force were applied. Conclusion: In the mouse model of orthodontic tooth movement, periodontal CTGF injection induces over expression of VEGF and accelerates the periodontal remodeling during orthodontic tooth movement.