CAJ | 학술논문

家蚕突变表型无鳞毛翅(non-lepis wing,nlw)由隐性基因nlw控制.由于家蚕雌性不发生交换,文章采用有鳞毛翅品系P50和无鳞毛翅品系U06两个品系组配F_1代及BC_1回交群体,(U06×P50)×U06和U06×(U06×P50)分别记作BC_1F和BC_1M,根据已经构建的家蚕SSR分子标记连锁图谱及已经发表的有关序列对nlw基因进行了连锁及定位分析.得到8个与nlw基因连锁的SSR(Simple sequence repeat)标记和1个STS(Sequence-taggedsites)标记.BC_1F群中的所有正常翅个体均表现出与(U06×P50)F_1相同的杂合带型;而所有无鳞毛个体带型与亲本U06一致,为纯合型.利用BC_1M群体构建了关于nlw基因的遗传连锁图,连锁图的遗传距离为125.7 cM,与nlw基因最近的引物为STS标记cash2p,图距为11.4 cM.
가잠돌변표형무린모시(non-lepis wing,nlw)유은성기인nlw공제.유우가잠자성불발생교환,문장채용유린모시품계P50화무린모시품계U06량개품계조배F_1대급BC_1회교군체,(U06×P50)×U06화U06×(U06×P50)분별기작BC_1F화BC_1M,근거이경구건적가잠SSR분자표기련쇄도보급이경발표적유관서렬대nlw기인진행료련쇄급정위분석.득도8개여nlw기인련쇄적SSR(Simple sequence repeat)표기화1개STS(Sequence-taggedsites)표기.BC_1F군중적소유정상시개체균표현출여(U06×P50)F_1상동적잡합대형;이소유무린모개체대형여친본U06일치,위순합형.이용BC_1M군체구건료관우nlw기인적유전련쇄도,련쇄도적유전거리위125.7 cM,여nlw기인최근적인물위STS표기cash2p,도거위11.4 cM.
The non-lepis wing of silkworm (Bombyx mori) is controlled by the recessive gene, nlw. Owning to lack of crossing over in females, the reciprocal backcrossed F_1 (BC_1) progenies were used for linkage analysis and mapping of nlw based on the SSR linkage map and STS markers using the wild type (+~(nlw)/+~(nlw)) silkworm strain P50 and U06 with scaleless wing (nlw/nlw). The nlw gene was linked to eight SSR markers and one STS marker. All the individuals with the wild type in the BC_1F (Using F_1 as female to backcross to the recessive parent, that is (U06×P50)xU06) showed heterozygous profile of (U06×P50) F_1, and the ones with non-lepis wing in BC_1F exhibited the homozygous profile of the strain U06. Using a reciprocal BC_1M (Using F_1 as male to backcross to the recessive parent, that is U06×(U06×P50))cross, we constructed a linkage map of 125.6 cM, and the distance between nlw and the nearest marker cash2p was 11.4 cM.