CAJ | 학술논문

目的从RNA水平探讨cyclinA1在野生型小鼠皮肤中的表达及意义.方法选取30只大于6个月的野生型小鼠,用原位杂交方法定位检测cyclinA1 mRNA在头颈部皮肤中的表达,同时以不加探针皮肤标本作为阴性对照,另取雄性小鼠睾丸组织作为阳性对照.结果分别有25只野生型小鼠在皮脂腺部位及12只在表皮全层有cyclinA1 mRNA表达.其中,皮脂腺部位强阳性及阳性表达分别占50.0%和33.3%,阳性率为83.3%;表皮全层强阳性及阳性表达分别占13.3%和26.7%,阳性率为40.0%.皮脂腺阳性率显著高于表皮(P<0.05).结论 CyclinA1 mRNA在野生型小鼠头颈部皮肤的皮脂腺部位及表皮全层的表达均有较高的阳性率,尤其皮脂腺表达的阳性率更高.
목적 종RNA수평탐토cyclinA1재야생형소서피부중적표체급의의.방법 선취30지대우6개월적야생형소서,용원위잡교방법정위검측cyclinA1 mRNA재두경부피부중적표체,동시이불가탐침피부표본작위음성대조,령취웅성소서고환조직작위양성대조.결과 분별유25지야생형소서재피지선부위급12지재표피전층유cyclinA1 mRNA표체.기중,피지선부위강양성급양성표체분별점50.0%화33.3%,양성솔위83.3%;표피전층강양성급양성표체분별점13.3%화26.7%,양성솔위40.0%.피지선양성솔현저고우표피(P<0.05).결론 CyclinA1 mRNA재야생형소서두경부피부적피지선부위급표피전층적표체균유교고적양성솔,우기피지선표체적양성솔경고.
Objective To study the expression and significance of cyclin A1 in the skin of wild-type mice at RNA level.Methods Thirty 6-12-week old wild-type Kunming mice(15 male and 15 female)were included in this study.In situ hybridization was used to detect the expression of cyclin A1 mRNA in the skin of head and neck of the mice.The skin treated without probe was regarded as negative control and the testis of male mice was taken as positive contr01.Results The expression of cyclin A1 mRNA was found in sebaceous glands of 25 mice and in epidermis of 12 mice.Strong positive staining of sebaceous glands was seen in 50% and positive staining in 33.3% of sections,whereas strong positive and positive staining of epidermis was seen in 13.3% and 20% of sections.respectively.The positive rate of sebaceous glands was 83.3%,much higher than that of epidermis(33.3%).Conclusions There is a quite high expression of eyelin A1 mRNA in the skin sebaceous gland and epidermis of head and neck of wild-type mice.Especially a strong expression is in the sebaceous glands.It indicates that cyclin A1 mRNA may play a certain role in the physiological function of sebaceous glands and epidermis of the skin.