CAJ | 학술논문

以抑制素A基因(Inhibin alpha subunit gene,INHA)作为辽宁绒山羊双羔性状的候选基因,以便为绒山羊育种工作中分子标记选择提供理论依据.运用测序法寻找辽宁绒山羊INHA基因单核苷酸多态性,筛查到一个新的多态位点-446TC,该位点位于INHA基因的启动子区域.运用PCR-RFLP法验证并分析该位点在辽宁绒山羊双羔群体和单羔群体间的多态性.结果表明,在辽宁绒山羊中双羔和单羔群体中分布A、B两个等位基因,处于中度多态.经χ~2检验,样本在该位点未达到Hardy-Weinberg平衡状态(P<0.05),不同基因型在辽宁绒山羊单、双胎群体上差异达到显著水平(P<0.05).
이억제소A기인(Inhibin alpha subunit gene,INHA)작위료녕융산양쌍고성상적후선기인,이편위융산양육충공작중분자표기선택제공이론의거.운용측서법심조료녕융산양INHA기인단핵감산다태성,사사도일개신적다태위점-446TC,해위점위우INHA기인적계동자구역.운용PCR-RFLP법험증병분석해위점재료녕융산양쌍고군체화단고군체간적다태성.결과표명,재료녕융산양중쌍고화단고군체중분포A、B량개등위기인,처우중도다태.경χ~2검험,양본재해위점미체도Hardy-Weinberg평형상태(P<0.05),불동기인형재료녕융산양단、쌍태군체상차이체도현저수평(P<0.05).
Inhibin alpha subunit (INHA) gene was studied as a candidate gene for the twinning trait of Liaoning cashmere goat in order to provide the theoretical basis for breeding work. A new SNP-446TC was detected by sequencing at promoter. Different genotypes were determined in Liaoning cashmere goat by restriction fragment length polymorphism (RFLP).The value of polymorphism information content indicated that this was a moderate polymorphism in Liaoning cashmere goat. The ?2 test indicated that the polymorphic locus in Liaoning cashmere goat did not fit Hardy-Weinberg equilibrium (P<0.05). Theχ~2 test of associational analysis between genotypic distribution and twinning or monotocous trait in Liaoning cashmere goat showed that the difference was significant (P<0.05).