国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2010年
17期
1034-1037
,共4页
支气管哮喘%血管内皮生长因子
支氣管哮喘%血管內皮生長因子
지기관효천%혈관내피생장인자
Bronchial asthma%Vascular endothelial growth factor
目的 探讨大鼠支气管哮喘(简称哮喘)模型支气管肺泡灌洗液(BALF)中血管内皮生长因子(VEGF)水平与哮喘嗜酸粒细胞(EOS)炎症及气道血管通透性之间的关系,以及吸入性激素的作用.方法 SD大鼠18只,随机分为对照组,哮喘模型组和地塞米松干预组各6只.以腹腔注射1%卵蛋白致敏和2%卵蛋白雾化吸入激发复制哮喘模型,干预组在每次激发前给予地塞米松干预.检测大鼠气道反应性,BALF中EOS百分数,VEGF(酶联免疫吸附法)及气道血管渗透指数.结果 BALF中EOS百分数,VEGF水平,气道反应性及气道血管渗透指数哮喘组明显高于对照组(P<0.05或P<0.01);经过6周吸入性激素治疗后,地塞米松组BALF中VEGF水平,EOS百分数,气道反应性及气道血管渗透指数较哮喘组明显降低(P<0.05或P<0.01),与对照组比较差异无统计学意义.相关分析显示,BALF中VEGF水平与EOS百分数呈正相关(r=0.76,P<0.01);VEGF水平与气道血管渗透指数呈正相关(r=0.84,P<0.01);VEGF水平与PC50呈负相关(r=-0.68,P<0.01).结论 大鼠哮喘模型BALF中VEGF水平增高,并与气道EOS百分数,气道反应性和气道血管渗透指数密切相关.该结果提示VEGF可能在哮喘的发病机制中起着重要作用.
目的 探討大鼠支氣管哮喘(簡稱哮喘)模型支氣管肺泡灌洗液(BALF)中血管內皮生長因子(VEGF)水平與哮喘嗜痠粒細胞(EOS)炎癥及氣道血管通透性之間的關繫,以及吸入性激素的作用.方法 SD大鼠18隻,隨機分為對照組,哮喘模型組和地塞米鬆榦預組各6隻.以腹腔註射1%卵蛋白緻敏和2%卵蛋白霧化吸入激髮複製哮喘模型,榦預組在每次激髮前給予地塞米鬆榦預.檢測大鼠氣道反應性,BALF中EOS百分數,VEGF(酶聯免疫吸附法)及氣道血管滲透指數.結果 BALF中EOS百分數,VEGF水平,氣道反應性及氣道血管滲透指數哮喘組明顯高于對照組(P<0.05或P<0.01);經過6週吸入性激素治療後,地塞米鬆組BALF中VEGF水平,EOS百分數,氣道反應性及氣道血管滲透指數較哮喘組明顯降低(P<0.05或P<0.01),與對照組比較差異無統計學意義.相關分析顯示,BALF中VEGF水平與EOS百分數呈正相關(r=0.76,P<0.01);VEGF水平與氣道血管滲透指數呈正相關(r=0.84,P<0.01);VEGF水平與PC50呈負相關(r=-0.68,P<0.01).結論 大鼠哮喘模型BALF中VEGF水平增高,併與氣道EOS百分數,氣道反應性和氣道血管滲透指數密切相關.該結果提示VEGF可能在哮喘的髮病機製中起著重要作用.
목적 탐토대서지기관효천(간칭효천)모형지기관폐포관세액(BALF)중혈관내피생장인자(VEGF)수평여효천기산립세포(EOS)염증급기도혈관통투성지간적관계,이급흡입성격소적작용.방법 SD대서18지,수궤분위대조조,효천모형조화지새미송간예조각6지.이복강주사1%란단백치민화2%란단백무화흡입격발복제효천모형,간예조재매차격발전급여지새미송간예.검측대서기도반응성,BALF중EOS백분수,VEGF(매련면역흡부법)급기도혈관삼투지수.결과 BALF중EOS백분수,VEGF수평,기도반응성급기도혈관삼투지수효천조명현고우대조조(P<0.05혹P<0.01);경과6주흡입성격소치료후,지새미송조BALF중VEGF수평,EOS백분수,기도반응성급기도혈관삼투지수교효천조명현강저(P<0.05혹P<0.01),여대조조비교차이무통계학의의.상관분석현시,BALF중VEGF수평여EOS백분수정정상관(r=0.76,P<0.01);VEGF수평여기도혈관삼투지수정정상관(r=0.84,P<0.01);VEGF수평여PC50정부상관(r=-0.68,P<0.01).결론 대서효천모형BALF중VEGF수평증고,병여기도EOS백분수,기도반응성화기도혈관삼투지수밀절상관.해결과제시VEGF가능재효천적발병궤제중기착중요작용.
Objective To investigate the relationship between vascular endothelial growth factor (VEGF) levels and eosinophilic inflammatory profiles,and the degree of airway vascular permeability in the asthmatic rats. We evaluated the effect of inhaled dexamethasone on VEGF levels in BALF. Methods Eighteen SD rats were randomly divided into three groups: the control group, the asthma model group and the dexamethasone group. Asthmatic model was established by sensitization and challenge with ovalbumin and rats were inhaled dexamethasone before challenge in dethamethasone group. The VEGF levels, the percentage of eosinophils,the airway vascular permeability index in BALF and the PC50 were examined.Results The VEGF levels,the percentage of eosinophils,the PC50 and the airway vascular permeability index in asthma model group were higher than that in control group( P <0.05 orP <0.01). After 6-week inhaled dexamethasone therapy,the VEGF levels, the percentage of eosinophils, the PC50 and the airway vascular permeability index in dexamethasone group were decreased compared to asthma model group( P<0.05 or P<0.01). The VEGF levels were negatively correlated with PC50, the percentage of eosinophils, the airway vascular permeability index( r = - 0. 68,0. 76,0. 84, P <0. 01). Conclusions The VEGF levels in BALF were increased in asthmatic rats and its levels were associated with airway percentage of EOS,airway responsiveness and airway vascular permeability. These findings provide strong evidence that VEGF may play an important role in pathogenesis of asthma.
