中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
7期
663-667
,共5页
刘静%杨洋%宫菊丽%张子宁%张旻%王亚男%姜拥军%戚中田%潘启超%钟平%尚红
劉靜%楊洋%宮菊麗%張子寧%張旻%王亞男%薑擁軍%慼中田%潘啟超%鐘平%尚紅
류정%양양%궁국려%장자저%장민%왕아남%강옹군%척중전%반계초%종평%상홍
丙型肝炎病毒%人免疫缺陷病毒%合并感染%基因亚型
丙型肝炎病毒%人免疫缺陷病毒%閤併感染%基因亞型
병형간염병독%인면역결함병독%합병감염%기인아형
Hepatitis C virus%HIV-1%Co-infection%Subtype
目的 了解中国部分地区HIV-1和HCV合并感染人群中HCV基因亚型的流行、分布及其与HIV-1感染疾病进展的关系.方法 对186份获自河南、云南、新疆、吉林和辽宁省HIV-1/HCV合并感染人群标本(HCV病毒载量>1000 cop/ml),用反转录巢式聚合酶链反应方法扩增血浆HCV核心基因区并进行基因亚型分型,同时检测HIV-1和HCV载量以及CD4+T细胞计数.结果 (1)HCV不同基因亚型比例分别为1a(1.7%)、1b(39.9%)、2a(17.9%)、3a(10.4%)、3b(15.6%)、6a(1.2%)、6n(6.4%)和6型未鉴定亚型(7.5%).HCV 2a和1b主要流行于河南省既往有偿献血浆人员中;3a和3b亚型主要流行于新疆和云南静脉注射吸毒者(IDU)中;HCV6型主要流行于云南吸毒人员中.(2)1b亚型的HCVRNA水平显著高于非1b亚型,但在HIV-1载量和CD4+T细胞数方面差异无统计学意义.2a亚型的HIV-1 RNA和HCV RNA水平显著低于非2a亚型.结论 HIV-1/HCV合并感染人群中HCV基因亚型的流行和分布与流行地区和感染途径有关.新的HCV6型亚型病毒株已经在合并感染的IDU人群中流行.尚未发现HCV基因哑型与HIV感染疾病进展之间的关系.
目的 瞭解中國部分地區HIV-1和HCV閤併感染人群中HCV基因亞型的流行、分佈及其與HIV-1感染疾病進展的關繫.方法 對186份穫自河南、雲南、新疆、吉林和遼寧省HIV-1/HCV閤併感染人群標本(HCV病毒載量>1000 cop/ml),用反轉錄巢式聚閤酶鏈反應方法擴增血漿HCV覈心基因區併進行基因亞型分型,同時檢測HIV-1和HCV載量以及CD4+T細胞計數.結果 (1)HCV不同基因亞型比例分彆為1a(1.7%)、1b(39.9%)、2a(17.9%)、3a(10.4%)、3b(15.6%)、6a(1.2%)、6n(6.4%)和6型未鑒定亞型(7.5%).HCV 2a和1b主要流行于河南省既往有償獻血漿人員中;3a和3b亞型主要流行于新疆和雲南靜脈註射吸毒者(IDU)中;HCV6型主要流行于雲南吸毒人員中.(2)1b亞型的HCVRNA水平顯著高于非1b亞型,但在HIV-1載量和CD4+T細胞數方麵差異無統計學意義.2a亞型的HIV-1 RNA和HCV RNA水平顯著低于非2a亞型.結論 HIV-1/HCV閤併感染人群中HCV基因亞型的流行和分佈與流行地區和感染途徑有關.新的HCV6型亞型病毒株已經在閤併感染的IDU人群中流行.尚未髮現HCV基因啞型與HIV感染疾病進展之間的關繫.
목적 료해중국부분지구HIV-1화HCV합병감염인군중HCV기인아형적류행、분포급기여HIV-1감염질병진전적관계.방법 대186빈획자하남、운남、신강、길림화요녕성HIV-1/HCV합병감염인군표본(HCV병독재량>1000 cop/ml),용반전록소식취합매련반응방법확증혈장HCV핵심기인구병진행기인아형분형,동시검측HIV-1화HCV재량이급CD4+T세포계수.결과 (1)HCV불동기인아형비례분별위1a(1.7%)、1b(39.9%)、2a(17.9%)、3a(10.4%)、3b(15.6%)、6a(1.2%)、6n(6.4%)화6형미감정아형(7.5%).HCV 2a화1b주요류행우하남성기왕유상헌혈장인원중;3a화3b아형주요류행우신강화운남정맥주사흡독자(IDU)중;HCV6형주요류행우운남흡독인원중.(2)1b아형적HCVRNA수평현저고우비1b아형,단재HIV-1재량화CD4+T세포수방면차이무통계학의의.2a아형적HIV-1 RNA화HCV RNA수평현저저우비2a아형.결론 HIV-1/HCV합병감염인군중HCV기인아형적류행화분포여류행지구화감염도경유관.신적HCV6형아형병독주이경재합병감염적IDU인군중류행.상미발현HCV기인아형여HIV감염질병진전지간적관계.
Objective To better understand the prevalence and geographic distribution of genotypes/subtypes on HCV and the relationship between HCV genotypes/subtypes and HIV infection disease progression in the HIV-1/HCV co-infected individuals living in high HIV-1 prevalent areas in China. Methods 186 plasma samples were collected from HIV-1 seropositive individuals infected through paid blood donors (PBD), injecting drug users (IDUs) or sexual contact, living in most severely affected provinces, Henan, Yunnan, Xinjiang, Jilin and Liaoning provinces. Samples with HCV viral load >1000 cop/ml were amplified by RT-nested PCR, sequenced and phylogenetically analyzed for genotyping/subtyping of HCV. HIV-1, HCV viral loads and CD4 T lymphocytes were measured for all subjects. Results (1) HCV were identified as 1 a (1.7%), 1 b (39.9%), 2a (17.9%), 3a (10.4%), 3b (15.6%), 6a (1.2%), 6n (6.4%), and a newly unclassified subtype (7.5%). HCV 2a and lb subtypes predominated in PBD in Henan, 3a and 3b in IDUs in Xinjiang and Yunnan, and 6 genotype/subtypes in IDU in Yunnan. (2) There were no significant differences in CD4 T cell counts among the different HCV subtypes. (3) The viral load of HCV RNA in lb subtype was higher than that of non-1b subtype, however, no significant differences in HIV-1 viral loads and CD4 T cell counts were found between Ib and non-1b subtype. Both HIV and HCV viral loads were lower in 2a than non-2a subtype. Conclusion The prevalence of HCV genotype/subtype in HIV-1/FICV co-infected individuals was associated with geographic areas and transmission routes. HCV subtypes had no direct correlation with HIV infection disease progression.
