中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
5期
554-557
,共4页
纪蓉%曹建平%陈遐林%朱巍%江庆%潘春燕%周媛媛%封阳%彭晓梅%刘杨%樊赛军
紀蓉%曹建平%陳遐林%硃巍%江慶%潘春燕%週媛媛%封暘%彭曉梅%劉楊%樊賽軍
기용%조건평%진하림%주외%강경%반춘연%주원원%봉양%팽효매%류양%번새군
放射增敏%青蒿琥酯%HeLa细胞%细胞凋亡
放射增敏%青蒿琥酯%HeLa細胞%細胞凋亡
방사증민%청호호지%HeLa세포%세포조망
Radiosensitization%Artesunate%HeLa cell%Apoptosis
目的 探讨表蒿琥酯对人宫颈癌HeLa细胞的放射增敏作用.方法 应用60Cor射线照射细胞,吸收剂量率为0.635 Gy/min,照射剂量为0、1、2、4和6 Gy.采用MTT法检测青蒿琥酯对HeLa细胞的抑制作用,确定青蒿琥酯的最适实验作用浓度.克隆形成法检测青蒿琥酯对HeLa细胞放射敏感性的影响;采用"多靶单击数学模型"拟合HeLa细胞的剂量-存活曲线,得出平均致死剂量、准阈剂量及放射增敏比,评价其增敏效果.用流式细胞术检测HeLa细胞凋亡率,进一步检测青蒿琥酯对HeLa细胞的放射增敏性.结果 青蒿琥酯对HeLa细胞的抑制作用随药物浓度的增加而增加,单纯照射1、2、4和6 Gy细胞的克隆形成率分别为91.67%、82.02%、58.60%和25.01%,加入青蒿琥酯后,相同照射剂量下克隆形成率分别降低为74.93%、60.53%、22.38%和5.05%;单纯照射组与药物+照射组的平均致死剂量(D0)分别为2.95和2.07 Gy,准阈剂量(Dq)分别为2.01和1.24 Gy,放射增敏比(SER)为1.43.单纯2和6 Gy照射组细胞凋亡率分别是12.26%和40.08%,加入青蒿琥酯后,相同照射剂量下细胞凋亡率分别上升至22.71%和59.92%.结论 青蒿琥酯对人宫颈癌HeLa细胞的抑制作用成药物浓度依赖性;青蒿琥酯对HeLa细胞具有一定的放射增敏作用.
目的 探討錶蒿琥酯對人宮頸癌HeLa細胞的放射增敏作用.方法 應用60Cor射線照射細胞,吸收劑量率為0.635 Gy/min,照射劑量為0、1、2、4和6 Gy.採用MTT法檢測青蒿琥酯對HeLa細胞的抑製作用,確定青蒿琥酯的最適實驗作用濃度.剋隆形成法檢測青蒿琥酯對HeLa細胞放射敏感性的影響;採用"多靶單擊數學模型"擬閤HeLa細胞的劑量-存活麯線,得齣平均緻死劑量、準閾劑量及放射增敏比,評價其增敏效果.用流式細胞術檢測HeLa細胞凋亡率,進一步檢測青蒿琥酯對HeLa細胞的放射增敏性.結果 青蒿琥酯對HeLa細胞的抑製作用隨藥物濃度的增加而增加,單純照射1、2、4和6 Gy細胞的剋隆形成率分彆為91.67%、82.02%、58.60%和25.01%,加入青蒿琥酯後,相同照射劑量下剋隆形成率分彆降低為74.93%、60.53%、22.38%和5.05%;單純照射組與藥物+照射組的平均緻死劑量(D0)分彆為2.95和2.07 Gy,準閾劑量(Dq)分彆為2.01和1.24 Gy,放射增敏比(SER)為1.43.單純2和6 Gy照射組細胞凋亡率分彆是12.26%和40.08%,加入青蒿琥酯後,相同照射劑量下細胞凋亡率分彆上升至22.71%和59.92%.結論 青蒿琥酯對人宮頸癌HeLa細胞的抑製作用成藥物濃度依賴性;青蒿琥酯對HeLa細胞具有一定的放射增敏作用.
목적 탐토표호호지대인궁경암HeLa세포적방사증민작용.방법 응용60Cor사선조사세포,흡수제량솔위0.635 Gy/min,조사제량위0、1、2、4화6 Gy.채용MTT법검측청호호지대HeLa세포적억제작용,학정청호호지적최괄실험작용농도.극륭형성법검측청호호지대HeLa세포방사민감성적영향;채용"다파단격수학모형"의합HeLa세포적제량-존활곡선,득출평균치사제량、준역제량급방사증민비,평개기증민효과.용류식세포술검측HeLa세포조망솔,진일보검측청호호지대HeLa세포적방사증민성.결과 청호호지대HeLa세포적억제작용수약물농도적증가이증가,단순조사1、2、4화6 Gy세포적극륭형성솔분별위91.67%、82.02%、58.60%화25.01%,가입청호호지후,상동조사제량하극륭형성솔분별강저위74.93%、60.53%、22.38%화5.05%;단순조사조여약물+조사조적평균치사제량(D0)분별위2.95화2.07 Gy,준역제량(Dq)분별위2.01화1.24 Gy,방사증민비(SER)위1.43.단순2화6 Gy조사조세포조망솔분별시12.26%화40.08%,가입청호호지후,상동조사제량하세포조망솔분별상승지22.71%화59.92%.결론 청호호지대인궁경암HeLa세포적억제작용성약물농도의뢰성;청호호지대HeLa세포구유일정적방사증민작용.
Objective To investigate the radiosensitizing effects of artesunate on human HeLa cells of cervical cancer in vitro.Methods Hela cells were irradiated with 60Co γ-rays.The dose rate was 0.635 Gy/min and the radiation dose was 0,1,2,4,6 Gy,respectively.The anti-proliferation activities of artesunate on HeLa cells were evaluated with MTT assay,to determine the most appropriate drug concentration.The effect of radiosensitivity was observed by using clonogenic assay.The single-hit multitarget model was used to plot the HeLa cell's dose-survival curve,to calculate mean lethal dose,quasithreshold dose and sensitization enhancement rate,and to evaluate its radiosensitization effect.The apoptosis was analyzed with flow cytometry (FCM) to further test the radiation senseitization of artesunate on HeLa cells.Results The inhibition of artesunate on HeLa cells increased with concentration.In radiation group,the cell cloning efficiency were 91.67% ,82.02% ,58.60% ,25.01%,respectively,and in artesunate (2.0 μ mol/L) + radiation group,the cell cloning efficiency were 74.93% ,60.53% ,22.38% ,5.05%.In radiation group and artesunate (2.0 μmol/L) + radiation group,the mean lethal dose(D0) was 2.95 and 2.07 Gy,respectively,while the qusai-threshold dose (Dq) were 2.01 and 1.24 Gy,respectively,and SER was 1.43.Compared with 2 and 6 Gy radiation group,the apoptosis rate of drug + radiation group increased from 12.26% ,40.08% to 22.71% ,59.92%.Conclusions The inhibiting effect of artesunate on HeLa cells is concentration-dependent.Artesunate has radiosensitizing effect on HeLa cells in vitro.
