中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2011年
1期
9-11,15
,共4页
詹瑞森%孙双喜%王卫国%陈世杰
詹瑞森%孫雙喜%王衛國%陳世傑
첨서삼%손쌍희%왕위국%진세걸
脊髓损伤/药物疗法%神经营养因子3/投药和剂量%神经生长因子类/拮抗剂和抑制剂/投药和剂量%抗体,单克隆/投药和剂量%脊髓/药物作用/代谢%原癌基因蛋白质c-fos/代谢%原癌基因蛋白质c-jun/代谢
脊髓損傷/藥物療法%神經營養因子3/投藥和劑量%神經生長因子類/拮抗劑和抑製劑/投藥和劑量%抗體,單剋隆/投藥和劑量%脊髓/藥物作用/代謝%原癌基因蛋白質c-fos/代謝%原癌基因蛋白質c-jun/代謝
척수손상/약물요법%신경영양인자3/투약화제량%신경생장인자류/길항제화억제제/투약화제량%항체,단극륭/투약화제량%척수/약물작용/대사%원암기인단백질c-fos/대사%원암기인단백질c-jun/대사
Spinal cord injuries/DT%Neurotrophin 3/AD%Nerve growth factors/AI/AD%Antibodies,monoclonal/AD%Spinal cord/DE/ME%Proto-oncogene proteins c-fos/ME%Proto-oncogene proteins cjun/ME
目的 探讨脊髓损伤后给予IN-1以及和NT-3联合作用后c-fos及c-jun的表达有无变化.方法 健康Sprague-Dawley(SD)大鼠120只,随机分为损伤对照组、IN-1组及IN-1和NT-3联合作用组.各组分别在相应时间点取材行RT-PCR检测c-fos和c-jun基因表达的变化.结果 IN-1作用后c-fos、c-jun表达分别出现下降和升高,联合应用NT-3后更为明显,其中c-fos的峰值手术对照组为0.974±0.126、IN-1组为0.834±0.047、联合作用组为0.698±0.052,c-jun的峰值手术对照组为0.642±0.048、IN-1组为0.712±0.050、联合作用组为0.814±0.041.结论 NT-3、IN-1作用机制之一可能通过抑制c-fos表达,促进c-jun表达对脊髓损伤起保护作用.
目的 探討脊髓損傷後給予IN-1以及和NT-3聯閤作用後c-fos及c-jun的錶達有無變化.方法 健康Sprague-Dawley(SD)大鼠120隻,隨機分為損傷對照組、IN-1組及IN-1和NT-3聯閤作用組.各組分彆在相應時間點取材行RT-PCR檢測c-fos和c-jun基因錶達的變化.結果 IN-1作用後c-fos、c-jun錶達分彆齣現下降和升高,聯閤應用NT-3後更為明顯,其中c-fos的峰值手術對照組為0.974±0.126、IN-1組為0.834±0.047、聯閤作用組為0.698±0.052,c-jun的峰值手術對照組為0.642±0.048、IN-1組為0.712±0.050、聯閤作用組為0.814±0.041.結論 NT-3、IN-1作用機製之一可能通過抑製c-fos錶達,促進c-jun錶達對脊髓損傷起保護作用.
목적 탐토척수손상후급여IN-1이급화NT-3연합작용후c-fos급c-jun적표체유무변화.방법 건강Sprague-Dawley(SD)대서120지,수궤분위손상대조조、IN-1조급IN-1화NT-3연합작용조.각조분별재상응시간점취재행RT-PCR검측c-fos화c-jun기인표체적변화.결과 IN-1작용후c-fos、c-jun표체분별출현하강화승고,연합응용NT-3후경위명현,기중c-fos적봉치수술대조조위0.974±0.126、IN-1조위0.834±0.047、연합작용조위0.698±0.052,c-jun적봉치수술대조조위0.642±0.048、IN-1조위0.712±0.050、연합작용조위0.814±0.041.결론 NT-3、IN-1작용궤제지일가능통과억제c-fos표체,촉진c-jun표체대척수손상기보호작용.
Objective To study the change of expression of c-fos and c-jun when using IN-1 alone or combination with NT-3 after spinal cord injury. Methods 120 adult health Sprague-Dawley (SD) rats were random divided into three groups, including control group, IN-1 group, and IN-1 combination with NT-3 group. All rats were killed at the scheduled time and its myeloid tissues were taken out. In each group, the expression of c-fos and c-jun gene was detected by using reverse transcription- polymerase chain reaction technique ( RT-PCR ). Result The transcriptional levels of c-fos decreased and c-jun increased when using IN-1 alone, and the levels changed more when using IN-1 combination with NT-3. The peak of c-fos reached to 0. 974 ±0. 126 in control group, 0. 834 ±0. 047 in IN-1 group, and 0. 698 ±0. 052 in IN-1 combination with NT-3 group, and the peak of c-jun reached 0. 642 ±0. 048, 0. 712 ±0. 050, and 0. 814 ±0. 041, respectively. Conclusion One of the mechanisms of IN-1 and NT-3 to protect the spinal cord might be through inhibiting the expression of c-fos and enhancing the expression of c-jun.
