肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2008年
10期
669-671
,共3页
崔东旭%魏晰麟%刘宝林%孙韶龙%张小薄%许维雪
崔東旭%魏晰麟%劉寶林%孫韶龍%張小薄%許維雪
최동욱%위석린%류보림%손소룡%장소박%허유설
肝肿瘤%肿瘤抑制蛋白质%p53%细胞.凋亡
肝腫瘤%腫瘤抑製蛋白質%p53%細胞.凋亡
간종류%종류억제단백질%p53%세포.조망
Liver neoplasms%Tumor suppressor protein%p53%Apoptosis,cell
目的 研究livin蛋白与突变型p53蛋白在肝癌组织中的表达和意义以及探讨两者间相关性.方法 采用免疫组织化学SP法检测80例肝癌、39例肝硬化、33例肝血管瘤旁正常肝组织中livin蛋白和突变型p53蛋白的表达,并通过显微图像分析方法进行结果观察.结果 livin蛋白的表达强度依次为肝癌组织(Median=5.09;P25～P75=3.06～8.28)、肝硬化组织(Median=3.05;P25～P25=2.49～4.25)、正常肝组织(Median=1.99;P25～P75=1.54～2.54)(P<0.001);突变型p53蛋白表达强度为肝癌组织(Median=43.13;P25～P75=20.41～78.53)高于肝硬化组织(Median=20.30;P25～P75=14.90～28.08)和正常肝组织(Median=15.52;P25～P75=12.81～21.80)(P<0.001),肝硬化组织与正常肝组织间差异无统计学意义.肝癌组织中livin蛋白与突变型p53蛋向的表达呈正相关(r=0.241,P<0.05).结论 肝癌组织中livin蛋白及突变型p53蛋白的高表达以及两者呈正相关,表明两者可能对肝癌的发生与发展起着协同作用.
目的 研究livin蛋白與突變型p53蛋白在肝癌組織中的錶達和意義以及探討兩者間相關性.方法 採用免疫組織化學SP法檢測80例肝癌、39例肝硬化、33例肝血管瘤徬正常肝組織中livin蛋白和突變型p53蛋白的錶達,併通過顯微圖像分析方法進行結果觀察.結果 livin蛋白的錶達彊度依次為肝癌組織(Median=5.09;P25～P75=3.06～8.28)、肝硬化組織(Median=3.05;P25～P25=2.49～4.25)、正常肝組織(Median=1.99;P25～P75=1.54～2.54)(P<0.001);突變型p53蛋白錶達彊度為肝癌組織(Median=43.13;P25～P75=20.41～78.53)高于肝硬化組織(Median=20.30;P25～P75=14.90～28.08)和正常肝組織(Median=15.52;P25～P75=12.81～21.80)(P<0.001),肝硬化組織與正常肝組織間差異無統計學意義.肝癌組織中livin蛋白與突變型p53蛋嚮的錶達呈正相關(r=0.241,P<0.05).結論 肝癌組織中livin蛋白及突變型p53蛋白的高錶達以及兩者呈正相關,錶明兩者可能對肝癌的髮生與髮展起著協同作用.
목적 연구livin단백여돌변형p53단백재간암조직중적표체화의의이급탐토량자간상관성.방법 채용면역조직화학SP법검측80례간암、39례간경화、33례간혈관류방정상간조직중livin단백화돌변형p53단백적표체,병통과현미도상분석방법진행결과관찰.결과 livin단백적표체강도의차위간암조직(Median=5.09;P25～P75=3.06～8.28)、간경화조직(Median=3.05;P25～P25=2.49～4.25)、정상간조직(Median=1.99;P25～P75=1.54～2.54)(P<0.001);돌변형p53단백표체강도위간암조직(Median=43.13;P25～P75=20.41～78.53)고우간경화조직(Median=20.30;P25～P75=14.90～28.08)화정상간조직(Median=15.52;P25～P75=12.81～21.80)(P<0.001),간경화조직여정상간조직간차이무통계학의의.간암조직중livin단백여돌변형p53단향적표체정정상관(r=0.241,P<0.05).결론 간암조직중livin단백급돌변형p53단백적고표체이급량자정정상관,표명량자가능대간암적발생여발전기착협동작용.
Objective To investigate the expression and relationship of livin and mutant p53 in hepatocellular carcinoma (HCC). Methods The expression of livin and mutant p53 were evaluated using the SP immunohistochemistry in 80 HCC tissue, 39 hepatic cirrhosis tissue, 33 normal tissue beside the hemangiomas of liver. Results The integrated A average results showed that the intension of positive expression of livin aligned by turns was HCC tissue (Median=5.09; P25～P75=3.06～8.28), hepatic cirrhosistissue(Median=3.05; P25～P75=2.49～4.25), normal liver tissue(Median=1.99; P25～P75=1.54～2.54) (P<0.001), respectively. It also showed that the intension of positive expression of p53 in HCC tissue (Median=43.13; P25～P75=20.41～78.53) was higher than that in hepatic cirrhosis tissue (Median=20.30;P25～P75=14.90～28.08), as well as in that of normal liver tissue (Median=15.52;P25～P75=12.81～21.80) (P <0.001), but it made no sense in statistics between hepatic cirrhosis tissue and normal tissue of liver. The expression of livin was obviously correlated with p53 in HCC tissue(r=0.241, P<0.05). Conclusion The overexpressian of livin and p53 and their positive correlation showed that livin may play a crucial role in the origin and development of HCC in coordination with p53.
