中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
6期
398-399
,共2页
成纤维细胞%胶原Ⅰ型%强脉冲光%mRNA表达
成纖維細胞%膠原Ⅰ型%彊脈遲光%mRNA錶達
성섬유세포%효원Ⅰ형%강맥충광%mRNA표체
Fibroblast%Collagen,type Ⅰ%Intense pulse light%mRNA expression
目的 研究强脉冲光(IPL)照射对人皮肤成纤维细胞Ⅰ型前胶原mRNA表达水平的影响.方法 用强脉冲光对目标皮肤进行连续照射,在照射前及照射后取照射部位皮肤组织,应用实时荧光定量PCR法测定各时间点成纤维细胞Ⅰ型前胶原mRNA的表达水平.结果 连续的强脉冲光照射可以显著提高Ⅰ型前胶原mRNA的表达水平.照射2,3次后皮肤成纤维细胞Ⅰ型前胶原mRNA表达[(7.9±1.7)×10-4、(11.1±2.4)×10-4]明显高于照射前[(2.1±0.7)×10-4],P＜0.05.结论 强脉冲光可促进皮肤成纤维细胞Ⅰ型前胶原mRNA的表达,证实了强脉冲光的除皱作用.
目的 研究彊脈遲光(IPL)照射對人皮膚成纖維細胞Ⅰ型前膠原mRNA錶達水平的影響.方法 用彊脈遲光對目標皮膚進行連續照射,在照射前及照射後取照射部位皮膚組織,應用實時熒光定量PCR法測定各時間點成纖維細胞Ⅰ型前膠原mRNA的錶達水平.結果 連續的彊脈遲光照射可以顯著提高Ⅰ型前膠原mRNA的錶達水平.照射2,3次後皮膚成纖維細胞Ⅰ型前膠原mRNA錶達[(7.9±1.7)×10-4、(11.1±2.4)×10-4]明顯高于照射前[(2.1±0.7)×10-4],P＜0.05.結論 彊脈遲光可促進皮膚成纖維細胞Ⅰ型前膠原mRNA的錶達,證實瞭彊脈遲光的除皺作用.
목적 연구강맥충광(IPL)조사대인피부성섬유세포Ⅰ형전효원mRNA표체수평적영향.방법 용강맥충광대목표피부진행련속조사,재조사전급조사후취조사부위피부조직,응용실시형광정량PCR법측정각시간점성섬유세포Ⅰ형전효원mRNA적표체수평.결과 련속적강맥충광조사가이현저제고Ⅰ형전효원mRNA적표체수평.조사2,3차후피부성섬유세포Ⅰ형전효원mRNA표체[(7.9±1.7)×10-4、(11.1±2.4)×10-4]명현고우조사전[(2.1±0.7)×10-4],P＜0.05.결론 강맥충광가촉진피부성섬유세포Ⅰ형전효원mRNA적표체,증실료강맥충광적제추작용.
Objective To investigate the effects of intense pulse light(IPL)on the mRNA expression of type Ⅰ procollagen in human fibroblasts.Methotis Four healthy female subjects aged 19-41,underwent IPL on the back skin 3 times with an interval of 2 weeks.Before the IPL and after 1,2,and 3 times of treatment samples of back skin were obtained to undergo real time quantitative PCR to detect the mRNA expression of type Ⅰ procollagen.Results The mRNA expression level of type Ⅰ procollagen after one time of IPL treatment was [(4.7±1.0)× 10-1],not significantly different from that before treatment [(2.1±0.7)×10-1,P=0.100].The mRNA expression levels of type Ⅰ proeollagen after treatment for 2 times and 3-time were(7.9±1.7)×10-4 and(11.1±2.4)×10-4 respectively,both significantly higher than that before treatment(both P＜0.05).Conclusion Promoting the mRNA transcription of type Ⅰ procollagen in dermal fibroblasts,IPL therapy is effective in dermal remodeling and wrinkle removing.
