CAJ | 학술논문

分析中国三种实验用小型猪线粒体DNA （mtDNA）D-loop的多态性，建立各品种品系猪的遗传标记，为各品种、品系猪的鉴别提供依据。应用PCR技术分别对西双版纳近交系小耳猪、广西巴马小型猪、贵州小型香猪和长白猪的血液总DNA样品中mtDNA D-loop进行扩增，用23种限制性内切酶消化，观察其酶切多态。PCR扩增其mtDNA D-loop 5′端227bp高变区域，应用PCR–SSCP和PCR直接测序分析，观察其单链构象多态和序列多态。结果显示：三种小型猪之间未见酶切长度多态、单链构象多态和序列多态。与长白猪之间表现出单链构象多态和序列多态。本研究认为：三种实验用小型猪之间mtDNA多态性贫乏，证明其亲源关系很近，在母系起源和进化上有一致性，应用PCR–RFLP、PCR–SSCP和PCR直接测序分析，尚不能作为三种实验用小型猪品种、品系鉴定的依据，但与长白猪等欧系猪比较有一定差异。
분석중국삼충실험용소형저선립체DNA （mtDNA）D-loop적다태성，건립각품충품계저적유전표기，위각품충、품계저적감별제공의거。응용PCR기술분별대서쌍판납근교계소이저、엄서파마소형저、귀주소형향저화장백저적혈액총DNA양품중mtDNA D-loop진행확증，용23충한제성내절매소화，관찰기매절다태。PCR확증기mtDNA D-loop 5′단227bp고변구역，응용PCR–SSCP화PCR직접측서분석，관찰기단련구상다태화서렬다태。결과현시：삼충소형저지간미견매절장도다태、단련구상다태화서렬다태。여장백저지간표현출단련구상다태화서렬다태。본연구인위：삼충실험용소형저지간mtDNA다태성빈핍，증명기친원관계흔근，재모계기원화진화상유일치성，응용PCR–RFLP、PCR–SSCP화PCR직접측서분석，상불능작위삼충실험용소형저품충、품계감정적의거，단여장백저등구계저비교유일정차이。
the present study is to analyze the polymorphism of the mtDNA D-loop in three breeds of laboratory miniature pigs in China , and to establish its cytoplasmic DNA markers to distinguish among them . The polymorphism of mtDNA D-loop and its 5′-end high variable regions were detected by PCR-RFLP , PCR-SSCP and PCR-direct sequencing on Xishuangbanna Small-ear inbred pig, Guizhou miniature Xiang pig , Guangxi Bama miniature pig and Landrace.There was no polymorphism obtained among or within three breeds of Chinese laboratory miniature pigs besides Landrace. It is concluded that the polymorphism of mtDNA D-loop within the three breeds of Chinese laboratory miniature pigs is poor , These methods cannot be used to distinguish among them , but it can be used to distinguish them from Landrace.