中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2005年
5期
985-989
,共5页
贾晓青%韩丽辉%钟宁%孟繁立%阎明%李文捷%李延青%张尚忠
賈曉青%韓麗輝%鐘寧%孟繁立%閻明%李文捷%李延青%張尚忠
가효청%한려휘%종저%맹번립%염명%리문첩%리연청%장상충
环加氧酶抑制剂%细胞凋亡%结肠肿瘤
環加氧酶抑製劑%細胞凋亡%結腸腫瘤
배가양매억제제%세포조망%결장종류
Cyclooxygenase inhibitors%Apoptosis%Colonic neoplasms
目的:研究NS-398(一种选择性环氧合酶-2抑制剂)对结肠癌HT-29细胞的生长抑制作用并探讨其机制. 方法: 通过MTT法检测细胞的增殖情况,通过流式细胞术检测细胞凋亡率和细胞周期,通过RT-PCR检测bcl-2 mRNA和bax mRNA表达,通过共聚焦激光扫描显微镜观察细胞骨架成分F-actin的变化. 结果: NS-398对结肠癌HT-29细胞生长的抑制具有时间和剂量依赖性.流式细胞术结果显示NS-398可剂量依赖地诱导HT-29细胞凋亡,使其停滞于G0/G1期.经不同浓度的NS-398处理72 h后,bcl-2 mRNA 在HT-29细胞的表达降低,bcl-2/bax比值降低.细胞骨架成分F-actin主要分布在HT-29细胞核周围,呈环状结构,NS-398作用后细胞核周围的环状结构消失. 结论: NS-398可显著抑制结肠癌细胞的体外生长并诱导其凋亡,这与下调bcl-2/bax比值以及细胞骨架的破坏有关.
目的:研究NS-398(一種選擇性環氧閤酶-2抑製劑)對結腸癌HT-29細胞的生長抑製作用併探討其機製. 方法: 通過MTT法檢測細胞的增殖情況,通過流式細胞術檢測細胞凋亡率和細胞週期,通過RT-PCR檢測bcl-2 mRNA和bax mRNA錶達,通過共聚焦激光掃描顯微鏡觀察細胞骨架成分F-actin的變化. 結果: NS-398對結腸癌HT-29細胞生長的抑製具有時間和劑量依賴性.流式細胞術結果顯示NS-398可劑量依賴地誘導HT-29細胞凋亡,使其停滯于G0/G1期.經不同濃度的NS-398處理72 h後,bcl-2 mRNA 在HT-29細胞的錶達降低,bcl-2/bax比值降低.細胞骨架成分F-actin主要分佈在HT-29細胞覈週圍,呈環狀結構,NS-398作用後細胞覈週圍的環狀結構消失. 結論: NS-398可顯著抑製結腸癌細胞的體外生長併誘導其凋亡,這與下調bcl-2/bax比值以及細胞骨架的破壞有關.
목적:연구NS-398(일충선택성배양합매-2억제제)대결장암HT-29세포적생장억제작용병탐토기궤제. 방법: 통과MTT법검측세포적증식정황,통과류식세포술검측세포조망솔화세포주기,통과RT-PCR검측bcl-2 mRNA화bax mRNA표체,통과공취초격광소묘현미경관찰세포골가성분F-actin적변화. 결과: NS-398대결장암HT-29세포생장적억제구유시간화제량의뢰성.류식세포술결과현시NS-398가제량의뢰지유도HT-29세포조망,사기정체우G0/G1기.경불동농도적NS-398처리72 h후,bcl-2 mRNA 재HT-29세포적표체강저,bcl-2/bax비치강저.세포골가성분F-actin주요분포재HT-29세포핵주위,정배상결구,NS-398작용후세포핵주위적배상결구소실. 결론: NS-398가현저억제결장암세포적체외생장병유도기조망,저여하조bcl-2/bax비치이급세포골가적파배유관.
AIM: To evaluate the growth-inhibitory effects of NS-398, a selective cyclooxygenase-2 inhibitor, in human colon cancer HT-29 cells and its possible mechanisms. METHODS: MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect apoptosis rate and cell cycle. RT-PCR was used to detect the expression of bcl-2 mRNA and bax mRNA. Alteration of cytoskeleton component F-actin was observed by confocal laser scanning microscope. RESULTS: NS-398 could inhibit growth of HT-29 cells in dose-and time-dependent manners. Flow cytometry revealed that NS-398 could induce apoptosis and cause G0/G1 arrest of HT-29 cells in a dose-dependent manner. After 72 h incubation with NS-398 at different concentrations, the expression level of bcl-2 mRNA was lowered and the ratio of bcl-2 to bax was decreased in HT-29 cells. F-actin was mainly distributed around nuclei forming annular structure in HT-29 cells. After exposure to NS-398, the annular structure around nuclei disappeared and fluorescence intensity of F-actin decreased obviously. CONCLUSION: NS-398 can inhibit the growth effectively and induce apoptosis in HT-29 cells in vitro, which is associated with the down-regulation of bcl-2 to bax ratio and the disruption of cytoskeleton.