CAJ | 학술논문

背景:丙二醛是脂质过氧化的终产物,丙二醛含量可以推断机体内的脂质过氧化损伤情况;谷胱甘肽过氧化物酶是生物机体内的自由基清除剂.而稳恒磁场对生物体的正、负面效应目前尚无定论.目的:探讨稳恒磁场对小鼠肝脏组织抗氧化能力的影响及其强度效应.设计:观察对比实验.单位:江苏大学医学物理实验室、生化实验室.材料:实验于2003-01/12在江苏大学医学物理实验室及生化实验室完成.选择昆明种小鼠30只,雌雄各半,体质量18～20 g.暴露装置采用铁氧体瓦形磁铁自制暴露盒.方法:将30只小鼠随机分成5组,正常对照组、磁感应强度(24.6±4.2),(42.0±2.1),(63.5±3.0),(85.1±2.9)mT组各6只.每天定时将正常对照组小鼠放入无磁场暴露盒内,磁感应强度(24.6±4.2),(42.0±2.1),(63.5±3.0),(85.1±2.9)mT组小鼠分别置于以上4种不同强度的稳恒磁场暴露盒内2次,2 h/次,15 d后麻醉处死小鼠,检测肝脏组织中谷胱甘肽过氧化物酶活性及丙二醛含量.主要观察指标:各组小鼠肝脏组织中丙二醛含量及谷胱甘肽过氧化物酶活性.结果:30只小鼠全部进入结果分析,无脱失.①小鼠肝脏组织中丙二醛含量:磁感应强度(24.6±4.2)mT,(42.0±2.1)mT组小鼠显著低于正常对照组[(12.70±0.53,12.96±0.72,17.62±0.91)μmol/g(F=10.4,9.89,P＜0.01)].②小鼠肝脏组织中谷胱甘肽过氧化物酶活性:磁感应强度(24.6±4.2)mT,(42.0±2.1)mT组小鼠显著高于正常对照组[(143.36±8.34,150.69±12.00,87.51±11.34)μkat/g(F=10.0,11.3,P＜0.01)].结论:一定磁感应强度的稳恒磁场能降低小鼠肝组织的丙二醛含量,增加谷胱甘肽过氧化物酶活性,提高抗氧化酶的活力,降低过氧化脂质的生成,减少其对生物体的损害,对延缓衰老有积极作用.
배경:병이철시지질과양화적종산물,병이철함량가이추단궤체내적지질과양화손상정황;곡광감태과양화물매시생물궤체내적자유기청제제.이은항자장대생물체적정、부면효응목전상무정론.목적:탐토은항자장대소서간장조직항양화능력적영향급기강도효응.설계:관찰대비실험.단위:강소대학의학물리실험실、생화실험실.재료:실험우2003-01/12재강소대학의학물리실험실급생화실험실완성.선택곤명충소서30지,자웅각반,체질량18～20 g.폭로장치채용철양체와형자철자제폭로합.방법:장30지소서수궤분성5조,정상대조조、자감응강도(24.6±4.2),(42.0±2.1),(63.5±3.0),(85.1±2.9)mT조각6지.매천정시장정상대조조소서방입무자장폭로합내,자감응강도(24.6±4.2),(42.0±2.1),(63.5±3.0),(85.1±2.9)mT조소서분별치우이상4충불동강도적은항자장폭로합내2차,2 h/차,15 d후마취처사소서,검측간장조직중곡광감태과양화물매활성급병이철함량.주요관찰지표:각조소서간장조직중병이철함량급곡광감태과양화물매활성.결과:30지소서전부진입결과분석,무탈실.①소서간장조직중병이철함량:자감응강도(24.6±4.2)mT,(42.0±2.1)mT조소서현저저우정상대조조[(12.70±0.53,12.96±0.72,17.62±0.91)μmol/g(F=10.4,9.89,P＜0.01)].②소서간장조직중곡광감태과양화물매활성:자감응강도(24.6±4.2)mT,(42.0±2.1)mT조소서현저고우정상대조조[(143.36±8.34,150.69±12.00,87.51±11.34)μkat/g(F=10.0,11.3,P＜0.01)].결론:일정자감응강도적은항자장능강저소서간조직적병이철함량,증가곡광감태과양화물매활성,제고항양화매적활력,강저과양화지질적생성,감소기대생물체적손해,대연완쇠로유적겁작용.
BACKGROUND: As the end product of lipid peroxidation, malondialdehyde (MDA) content can be used for assessment lipid peroxidation injury.Glutathione peroxidase (GSH-Px) acts as a free radical scavenger. Currently the effect of static magnetic field on the organism, whether positive or negative, has not been elucidated.OBJECTIVE: To study the effect of static magnetic field on anti-oxidation capacity of mouse hepatic tissues and its intensity dependence for producing such effects.DESIGN: A controlled comparative experiment.SETTING: Laboratories of Medical Physics and Biochemistry of Jiangsu University.MATERIALS: The experiment was conducted in the Laboratories of Medical Physics and Biochemistry of Jiangsu University from January to December 2003. Totally 30 mice of either sex weighing 18-20 g were selected and subjected to magnetic filed exposure using a self-designed ferrite magnet apparatus.METHODS: The mice were equally randomized into normal control group and 4 exposure groups exposed to magnetic field of (24.6±4.2) mT,(42.0±2.1) mT, (63.5±3.0) mT, and (85.1±2.9) mT, respectively. The mice in the 4 exposure groups were exposed to static magnetic field of the specified intensity for 2 hours twice a day, while those in the normal control group were subjected to the sham exposure apparatus without magnetic field at scheduled time points every day. After 15 days of exposure, the mice were sacrificed and the GSH-Px activity and the MDA content in the hepatic tissue were assayed.MAIN OUTCOME MEASURES: GSH-Px activity and MDA content in hepatic tissue of the mice.RESULTS: Thirty mice entered the final analysis without losses. MDA content in (24.6±4.2) mT and (42.0±2.1) mT groups were obviously lower than that in the normal control group [(12.70±0.53), (12.96±0.72), and (17.62±0.91) μmol/g, respectively, F=10.4, 9.89, P ＜ 0.01]. The GSH-Px activity in the hepatic tissue in (24.6±4.2) mT and (42.0±2.1) mT groups were obviously higher than that in the normal control group [(143.36±8.34),(150.69±12.00), (87.51±11.34) μkat/g, respectively, F=10.0, 11.3, P ＜ 0.01].CONCLUSION: Static magnetic field of appropriate intensity can lower MDA content and enhance the GSH-Px activity in the hepatic tissue of mice, and may also improve the activity of antioxidase and reduce the production of lipid peroxidation to diminish the consequent injuries and delay the aging process.