CAJ | 학술논문

目的比较裂解型分枝杆菌噬菌体检测技术(Phage amplified biologically assay,PhaB)和荧光定量聚合酶链反应(FQ-PCR)快速检测临床标本中结核分枝杆菌的优缺点及临床应用价值.方法对142例肺结核患者的痰标本和41份非结核性痰标本应用PhaB法和FQ-PCR进行检测和结果比较.结果 PhaB法阳性76例,阳性率53.5%;FQ-PCR法阳性84例,阳性率59.1%.PhaB法和FQ-PCR法相比,有统计学差异(P<0.05).2种方法检测41份非结核性痰标本均为阴性.结论两种方法能快速、简便、灵敏、特异检测结核分枝杆菌,同时又有不同的特点.
목적 비교렬해형분지간균서균체검측기술(Phage amplified biologically assay,PhaB)화형광정량취합매련반응(FQ-PCR)쾌속검측림상표본중결핵분지간균적우결점급림상응용개치.방법 대142례폐결핵환자적담표본화41빈비결핵성담표본응용PhaB법화FQ-PCR진행검측화결과 비교.결과 PhaB법양성76례,양성솔53.5%;FQ-PCR법양성84례,양성솔59.1%.PhaB법화FQ-PCR법상비,유통계학차이(P<0.05).2충방법 검측41빈비결핵성담표본균위음성.결론 량충방법 능쾌속、간편、령민、특이검측결핵분지간균,동시우유불동적특점.
Objective To compare the sensitivity and specificity by using mycobacteriophage splitting assay (PhaB) and fluorescence quantitative PCR (FQ-PCR), and evaluate the application value in clinical detection of Mycobacterium tuberculosis. Method The 142 sputum specimens of pulmonary tuberculosis,41 sputum specimens of non-tuberculosis were detected by PhaB and FQ-PCR assay; the obtained results were compared each other. Rseults of the 142 sputum specimens of pulmonary tuberculosis, 76 (53.5%) cases were confirmed as positive by using PhaB, 84 (59.1%) cases positive by FQ-PCR assay.The statistically significant difference of positive rate was found between PhaB and FQ-PCR assay (P<0.05); 41 sputum specimens of nontubereulosis were confirmed as negative by the two assays.Conclusion The PhaB and FQ-PCR assays have high sensibility and specifictity, but each has it-self different dominance.