CAJ | 학술논문

目的探讨重组人血管抑制因子Vasostatin(120-180aa)对于碱烧伤诱导的家兔角膜新生血管的抑制作用.方法采用1 mol/L NaOH溶液烧伤家兔角膜,建立碱烧伤诱导的家兔角膜新生血管的动物模型;将家兔分为A、B、C、D 4组,每组10只眼.伤后24 h后分别给予1×PBS缓冲液,20、40、80μg/mL Vasostatin(120-180aa)球结膜下注射,2次/周,共4周.测量各时间点角膜新生血管的生长面积,观察血管的生长情况.结果各时间点角膜新生血管的面积,B组、C组及D组均低于A组(P<0.05),C组及D组均低于B组(P<0.05),C组与D组相比,差异无显著性(P>0.05).结论重组人Vasostatin(120-180 aa)蛋白可有效抑制角膜新生血管的形成.
목적 탐토중조인혈관억제인자Vasostatin(120-180aa)대우감소상유도적가토각막신생혈관적억제작용.방법 채용1 mol/L NaOH용액소상가토각막,건립감소상유도적가토각막신생혈관적동물모형;장가토분위A、B、C、D 4조,매조10지안.상후24 h후분별급여1×PBS완충액,20、40、80μg/mL Vasostatin(120-180aa)구결막하주사,2차/주,공4주.측량각시간점각막신생혈관적생장면적,관찰혈관적생장정황.결과 각시간점각막신생혈관적면적,B조、C조급D조균저우A조(P<0.05),C조급D조균저우B조(P<0.05),C조여D조상비,차이무현저성(P>0.05).결론 중조인Vasostatin(120-180 aa)단백가유효억제각막신생혈관적형성.
Objective To investigate the inhibitory effect of recombinant human vasostatin (120-180aa) on alkaline-induced corneal neovascularization.Methods Corneal neovascularization animal model was established using 1 mol/L NaOH solution in rabbit.The rabbit eyes were divided into 4 groups:A group (10 eyes),B group (10 eyes),C group (10 eyes) and D group (10 eyes).1×PBS solution buffer,20,40 and 80 μg/mL vasostatin(120-180 aa) protein was subconjunctivally injected respectively in rabbits 24 hours after burning for twice a week.The areas of rabbit corneal neovascularization were measured at each time point.Results The areas of corneal neovascularization in B group,C group and D group were significantly lower than that A group at each time point(P<0.05),and those of C group and D group were lower than B group (P<0.05),but there was not significant difference between C group and D group (P>0.05).Conclusion The recombinant human vasostatin(120-180 aa) can effectively inhibit alkaline-induced corneal neovascularization.