环境工程学报
環境工程學報
배경공정학보
CHINESE JOURNAL OF ENVIRONMENTAL ENGINEERING
2009年
8期
1529-1531
,共3页
杨郁%金志华%李宁慧%金飞玲%黄翔和
楊鬱%金誌華%李寧慧%金飛玲%黃翔和
양욱%금지화%리저혜%금비령%황상화
甲醛%分离鉴定%16S%rDNA%PCR
甲醛%分離鑒定%16S%rDNA%PCR
갑철%분리감정%16S%rDNA%PCR
formaldehyde%isolation and identification%16S rDNA%PCR
从某化工厂污水处理系统的活性污泥中分离纯化得到一株能降解甲醛的革兰氏阳性菌,对其进行形态及生理生化特性测定,提取并纯化其基因组DNA,通过PCR扩增菌株的16S rDNA,测序结果表明,该菌株和苏云金芽孢杆菌的同源性为100%.在含1.2 μL/mL的甲醛的培养基上培养该菌36 h后,甲醛降解去除率达到72%以上.
從某化工廠汙水處理繫統的活性汙泥中分離純化得到一株能降解甲醛的革蘭氏暘性菌,對其進行形態及生理生化特性測定,提取併純化其基因組DNA,通過PCR擴增菌株的16S rDNA,測序結果錶明,該菌株和囌雲金芽孢桿菌的同源性為100%.在含1.2 μL/mL的甲醛的培養基上培養該菌36 h後,甲醛降解去除率達到72%以上.
종모화공엄오수처리계통적활성오니중분리순화득도일주능강해갑철적혁란씨양성균,대기진행형태급생리생화특성측정,제취병순화기기인조DNA,통과PCR확증균주적16S rDNA,측서결과표명,해균주화소운금아포간균적동원성위100%.재함1.2 μL/mL적갑철적배양기상배양해균36 h후,갑철강해거제솔체도72%이상.
A gram positive bacteria strain that can degrade formaldehyde was isolated from activated sludge of wastewater treatment plant from a chemical corporation.The strain was observed through structure and physio-logical and biochemical characteristics.Molecule identification methods including distilled DNA.PCR amplifiea-tion,production sequence showed that it was similar to Bacillus and 16S rDNA sequence shared 100%homology with that of Bacillus thuringiensis.Formaldehyde(1.2 μL/mL)was degraded above 72% with 36 h by this strain.
