中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2009年
10期
901-904
,共4页
沈敏鹤%阮善明%鲍旻奂%许家鸾%王琦%黄静
瀋敏鶴%阮善明%鮑旻奐%許傢鸞%王琦%黃靜
침민학%원선명%포민환%허가란%왕기%황정
水中草颗粒%复发性口腔溃疡%T淋巴细胞%白介素-10%白介素-12
水中草顆粒%複髮性口腔潰瘍%T淋巴細胞%白介素-10%白介素-12
수중초과립%복발성구강궤양%T림파세포%백개소-10%백개소-12
Shuizhongcao Granule%recurrent aphthous stomatitis%T-lymphocyte%interleukin 10%interleukin 12
目的 探讨水中草颗粒对复发性口腔溃疡细胞免疫功能的影响.方法 采用免疫方法建立复发性口腔溃疡动物模型,将成模的复发性口腔渍疡家兔随机分为模型组、左旋咪唑组(2.5 mg/mL)、水中草低剂量组(0.25 g/mL)和高剂量组(0.5 g/mL),同时设立正常组.从实验的第7周开始,左旋咪唑组、水中草低剂量组及水中草高剂量组每天分别以相应药物灌胃,共连续28天;取耳缘静脉血栓测T淋巴细胞亚群(CD_3、CD_4、CD_8)、血清白细胞介素(IL)-10和IL-12的水平.结果 模型组家兔CD_3、CD_4及CD_4/CD_8比值[分别为(40.50±7.46)%、(30.80±5.33)%及79.56±8.32]均较正常组显著下降(P<0.05),血清IL-10水平[(8.02±0.53)ng/L]较正常组升高(P<0.05),IL-12水平[(15.51±1.35)ng/L]较正常组下降(P<0.05);血清CD_3、CD_4、CD_4/CD_8比值及IL-12水平,左旋咪唑[(55.30±6.33)%、(43.50±5.33)%、99.29±13.84及(17.72±1.70)ng/L]、水中草低剂量[(58.50±6.03)%、(42.80±3.17)%、110.27±12.85及(17.74±1.96)ng/L]、水中草高剂量组[(61.50±4.32)%、(47.20±4.37)%、103.30±7.42及(18.18±1.54)ng/L]均较模型组升高(P<0.05,P<0.01),血清IL-10水平左旋咪唑组、水中草低剂量组和水中草高剂量组均较模型组降低(P<0.05,P<0.01).结论 复发性口腔溃疡家兔免疫功能处于抑制状态,水中草颗粒能够改善复发性口腔溃疡家兔的免疫抑制状态,达到治疗复发性口腔溃疡的作用.
目的 探討水中草顆粒對複髮性口腔潰瘍細胞免疫功能的影響.方法 採用免疫方法建立複髮性口腔潰瘍動物模型,將成模的複髮性口腔漬瘍傢兔隨機分為模型組、左鏇咪唑組(2.5 mg/mL)、水中草低劑量組(0.25 g/mL)和高劑量組(0.5 g/mL),同時設立正常組.從實驗的第7週開始,左鏇咪唑組、水中草低劑量組及水中草高劑量組每天分彆以相應藥物灌胃,共連續28天;取耳緣靜脈血栓測T淋巴細胞亞群(CD_3、CD_4、CD_8)、血清白細胞介素(IL)-10和IL-12的水平.結果 模型組傢兔CD_3、CD_4及CD_4/CD_8比值[分彆為(40.50±7.46)%、(30.80±5.33)%及79.56±8.32]均較正常組顯著下降(P<0.05),血清IL-10水平[(8.02±0.53)ng/L]較正常組升高(P<0.05),IL-12水平[(15.51±1.35)ng/L]較正常組下降(P<0.05);血清CD_3、CD_4、CD_4/CD_8比值及IL-12水平,左鏇咪唑[(55.30±6.33)%、(43.50±5.33)%、99.29±13.84及(17.72±1.70)ng/L]、水中草低劑量[(58.50±6.03)%、(42.80±3.17)%、110.27±12.85及(17.74±1.96)ng/L]、水中草高劑量組[(61.50±4.32)%、(47.20±4.37)%、103.30±7.42及(18.18±1.54)ng/L]均較模型組升高(P<0.05,P<0.01),血清IL-10水平左鏇咪唑組、水中草低劑量組和水中草高劑量組均較模型組降低(P<0.05,P<0.01).結論 複髮性口腔潰瘍傢兔免疫功能處于抑製狀態,水中草顆粒能夠改善複髮性口腔潰瘍傢兔的免疫抑製狀態,達到治療複髮性口腔潰瘍的作用.
목적 탐토수중초과립대복발성구강궤양세포면역공능적영향.방법 채용면역방법건립복발성구강궤양동물모형,장성모적복발성구강지양가토수궤분위모형조、좌선미서조(2.5 mg/mL)、수중초저제량조(0.25 g/mL)화고제량조(0.5 g/mL),동시설립정상조.종실험적제7주개시,좌선미서조、수중초저제량조급수중초고제량조매천분별이상응약물관위,공련속28천;취이연정맥혈전측T림파세포아군(CD_3、CD_4、CD_8)、혈청백세포개소(IL)-10화IL-12적수평.결과 모형조가토CD_3、CD_4급CD_4/CD_8비치[분별위(40.50±7.46)%、(30.80±5.33)%급79.56±8.32]균교정상조현저하강(P<0.05),혈청IL-10수평[(8.02±0.53)ng/L]교정상조승고(P<0.05),IL-12수평[(15.51±1.35)ng/L]교정상조하강(P<0.05);혈청CD_3、CD_4、CD_4/CD_8비치급IL-12수평,좌선미서[(55.30±6.33)%、(43.50±5.33)%、99.29±13.84급(17.72±1.70)ng/L]、수중초저제량[(58.50±6.03)%、(42.80±3.17)%、110.27±12.85급(17.74±1.96)ng/L]、수중초고제량조[(61.50±4.32)%、(47.20±4.37)%、103.30±7.42급(18.18±1.54)ng/L]균교모형조승고(P<0.05,P<0.01),혈청IL-10수평좌선미서조、수중초저제량조화수중초고제량조균교모형조강저(P<0.05,P<0.01).결론 복발성구강궤양가토면역공능처우억제상태,수중초과립능구개선복발성구강궤양가토적면역억제상태,체도치료복발성구강궤양적작용.
Objective To investigate the effect of Shuizhongcao Granule(SZCG)on cellular immune function in animal model of recurrent aphthous stomatitis(RAS).Methods Experimental animal model of RAS were established by immunological method.The modeled animals were randomized into 4 groups,Group A was the model control group,Group B was treated by Levamisole(2.5 mg/mL),Group C and D was treated with high-dose(0.5 g/mL)and low-dose(0.24 g/mL)SZCG respectively.The medication was administered via gastric perfusion,starting from the 7th week of the experiment,and continued for 28 days.Meanwhile,a normal control group was set up.Percentage of T-lymphocyte subsets,CD_3,CD_4 and CD_8,and serum levels of interleukin 10 and 12(IL-10 and IL-12)in peripheral blood were detected before and after treatment.Results Percentages of CD_3,CD_4 and CD_4/CD_8 ratio in the model control group were 40.50±7.46%,30.80±5.33% and 79.56±8.32 respectively,all lower than those in the normal control group(P<0.05);level of serum IL-10 was higher(8.02±0.53 ng/L)and that of IL-12 was lower(15.51±1.35 ng/L)in the model animal than those in normal control(P<0.05).After treatment,levels of CD_3,CD_4,CD_4/CD_8 and IL-12 in Group B were 55.30±6.33%,43.50±5.33%,99.29±13.84 and 17.72±1.70 ng/L,those in Group C were 61.50±4.32%,47.20±4.37%,103.30±7.42 and 18.18±1.54 ng/L,and in Group D were 58.50±6.03%,42.80±3.17%,110.27±12.85 and 17.74±1.96 ng/L,respectively,all were higher than those in Group A,but levels of IL-10 in the three treatment groups were lower than that in Goup A(P<0.05 or P<0.01).Conclusion The immune function of RAS model animals is in the suppressed condition;SZCG can improve the immune suppression to achieve its therapeutic effect on the disease.
