CAJ | 학술논문

[目的]探讨生黄芩饮片黄酮类化合物的纤维素酶辅助提取优化工艺条件.[方法]采用系列单因素和正交试验设计,通过高效液相分析以黄酮苷转化成的苷元含量为考核指标,对影响辅助提取的因素进行了研究,并与传统水煎煮法比较.[结果]生黄芩黄酮苷元的最佳纤维素酶辅助提取工艺为液固比10∶ 1,酶浓度25 U/g(药材),pH值5.0,温度50 ℃,提取时间7 h.最佳提取条件下,黄酮苷元黄芩素和汉黄芩素的提取率分别为175.04、28.84 μmol/g(药材),分别为传统水煎煮法的6.7倍和4.8倍.[结论]纤维素酶辅助提取法提取黄芩素和汉黄芩素等转化率高,易于实现规模化生产.
[목적]탐토생황금음편황동류화합물적섬유소매보조제취우화공예조건.[방법]채용계렬단인소화정교시험설계,통과고효액상분석이황동감전화성적감원함량위고핵지표,대영향보조제취적인소진행료연구,병여전통수전자법비교.[결과]생황금황동감원적최가섬유소매보조제취공예위액고비10∶ 1,매농도25 U/g(약재),pH치5.0,온도50 ℃,제취시간7 h.최가제취조건하,황동감원황금소화한황금소적제취솔분별위175.04、28.84 μmol/g(약재),분별위전통수전자법적6.7배화4.8배.[결론]섬유소매보조제취법제취황금소화한황금소등전화솔고,역우실현규모화생산.
[Objective] The research aimed to study the optimum extraction technique of flavonoid aglycons from skullcap root by cellulase-assisted extraction method. [Method] The conditions for the extraction by cellulase-assisted extraction method were studied through several single factor tests and the orthogonal experimental design, as an evaluation index of the content of baicalein and wogonin in skullcap root by HPLC method. The cellulase-assisted extraction method was compared with the water-decocting method. [Result] The optimized conditions of cellulase-assisted extraction are as follows: liquid-sold ratio is 10∶ 1, the concentration of cellulase is 25 U/g herbals, pH value is 5.0, extraction temperature is 50 ℃, and extraction time is 7.0 hours. Under the optimal conditions, the extraction rate of baicalein and wogonin reached 175.04 and 28.84 μmol/g herbals respectively, being 6.7 times and 4.8 times as that of the conventional water-decocting method. [Conclusion] Cellulase-assisted extraction method for the flavonoid aglycons in skullcap root was efficient, appropriate and feasible.