CAJ | 학술논문

背景与目的:研究表明,黄连素在清热解毒、抗菌消炎的同时,还具有诱导肿瘤细胞凋亡的作用,但最近的研究认为黄连素还能抑制肿瘤细胞的转移和扩散.为了进一步探讨黄连素对肿瘤细胞转移的作用及机制,本研究对黄连素作用后的肺腺癌A549细胞的增殖、迁移及黏附能力进行了检测.方法:应用MTT法检测不同浓度黄连素作用后细胞增殖情况;通过划痕试验和黏附试验测定黄连素作用前后细胞迁移和黏附能力;半定量RT-PCR法检测MMP2、MMP9的mRNA表达;明胶酶谱法测定MMP2、MMP9的活性.结果:黄连素能显著抑制A549增殖,并呈现一定的量效和时效关系(P<0.05);细胞的迁移率和黏附率明显降低,并且呈现剂量依赖性(P<0.05);经黄连素作用后的A549细胞,MMP2和MMP9的mRNA的表达明显降低,MMP2和MMP9降解明胶的能力下降,尤以100 μg/mL的作用最为明显(P<0.05).结论:黄连素能够抑制A549细胞的迁移和黏附能力,其机制可能与下调MMP2和MMP9的mRNA表达,从而降低其活性有关.
배경여목적:연구표명,황련소재청열해독、항균소염적동시,환구유유도종류세포조망적작용,단최근적연구인위황련소환능억제종류세포적전이화확산.위료진일보탐토황련소대종류세포전이적작용급궤제,본연구대황련소작용후적폐선암A549세포적증식、천이급점부능력진행료검측.방법:응용MTT법검측불동농도황련소작용후세포증식정황;통과화흔시험화점부시험측정황련소작용전후세포천이화점부능력;반정량RT-PCR법검측MMP2、MMP9적mRNA표체;명효매보법측정MMP2、MMP9적활성.결과:황련소능현저억제A549증식,병정현일정적량효화시효관계(P<0.05);세포적천이솔화점부솔명현강저,병차정현제량의뢰성(P<0.05);경황련소작용후적A549세포,MMP2화MMP9적mRNA적표체명현강저,MMP2화MMP9강해명효적능력하강,우이100 μg/mL적작용최위명현(P<0.05).결론:황련소능구억제A549세포적천이화점부능력,기궤제가능여하조MMP2화MMP9적mRNA표체,종이강저기활성유관.
Background and purpose: Reports showed that berbefine not only had an effect ofdetoxification and inflammation but also could induce the cell to apoptosis. Berberine may also inhibit the migration and metastasis of tumor. To investigate the effects and mechanism of berberine on migration and metastasis of A549 cells, the proliferation, mobility and adhesion of A549 cells were observed after incubation with berberine. Methods: The proliferation was determined by MTT. Wound healing assay and adhesion test were used to observe the mobility and adhesion rate. The mRNA and protein expressions of MMP2 and MMP9 were assessed by semi-quantitive RT-PCR and gelatin zymography, respectively. Results: Berberine could significantly inhibit the proliferation ofA549 within a certain range of treating time and dose (P<0.05). The mRNA and protein expression of MMP2 and MMP9 gene were decreased slightly by the treatment ofberberine (P<0.05), especially at dose of 100 μg/ml. Conclusion: Berberine can inhibit the migration and adhesion abilities of A549 cells, probably by down-regulating the expression and activation of MMP2 and MMP9.