CAJ | 학술논문

Trichoder VI是拟康氏木霉SMF2(Trichoderma pseudokoningii SMF2)产生的一种含有20个氨基酸的peptaibol类抗菌肽.采用圆二色谱法研究了不同极性溶剂体系和膜拟态环境对Trichokonin Ⅵ的二级结构的影响,Trichokonin Ⅵ在不同有机溶剂体系中具有典型的螺旋结构特征,而在水溶液体系中螺旋明显减少,不超过30%的三氟乙醇可以随浓度增加而诱导螺旋结构的增加.Trichokonin Ⅵ在磷酸缓冲液中峰的强度随浓度增加而增强,具有浓度依赖性.SDS浓度的增加可以诱导螺旋含最的增加,与非拟态环境相比,膜拟态环境可以明显增加Trichokonin Ⅵ的螺旋含量,表明Trichokonin Ⅵ可以和脂膜相互作用.
Trichoder VI시의강씨목매SMF2(Trichoderma pseudokoningii SMF2)산생적일충함유20개안기산적peptaibol류항균태.채용원이색보법연구료불동겁성용제체계화막의태배경대Trichokonin Ⅵ적이급결구적영향,Trichokonin Ⅵ재불동유궤용제체계중구유전형적라선결구특정,이재수용액체계중라선명현감소,불초과30%적삼불을순가이수농도증가이유도라선결구적증가.Trichokonin Ⅵ재린산완충액중봉적강도수농도증가이증강,구유농도의뢰성.SDS농도적증가가이유도라선함최적증가,여비의태배경상비,막의태배경가이명현증가Trichokonin Ⅵ적라선함량,표명Trichokonin Ⅵ가이화지막상호작용.
Trichokonin VI,a peptaibol-like antimicrobial peptides isolated from the cultured substrates of trichoderma koningii SMF_2,has 20 amino acid residues.The conformational flexibility of trichokonin Ⅵ in organic solvents with different polarities,aqueous solvents and membrane mimic solvents was studied by circular dichroism spectroscopy.Trichokonin Ⅵ takes on a typical a-helical structure in different organic solvents,but helicity decreases in aqueous solvent.The helical content increases with increasing the concentration of TFE up to 30%.In phosphate buffered saline,the CD spectrum of trichokonin Ⅵ is concentration dependent,and the intensity of the peaks increases with increasing the concentration of trichokonin VI.SDS induces a significant transition towards a helix formation,and the CD spectra in membrane mimic solvents increase helicity compared with those recorded without membrane mimic solvents,suggesting the interaction of the peptides with the membrane.