CAJ | 학술논문

背景与目的:侵袭转移是导致结肠癌死亡率增加的主要原因,如何阻断肿瘤的侵袭转移成为目前研究的热点.本研究旨在检测Syndecan-1基因和HPA-1基因在结肠癌组织中的表达水平,探讨二者与结肠癌侵袭转移及预后的关系.方法:用实时荧光定量逆转录聚合酶链反应(RT-PCR)分别检测49例结肠癌、49例配对的癌旁组织(距癌2cm)和49例配对远离结肠癌的手术切缘正常组织(距癌5cm以上)的Syndecan-1和HPA-1基因的表达水平,分析二者与结肠癌临床病理特征的关系.结果:结肠癌中HPA-1mRNA的表达水平(40.56±11.75)显著高于癌旁组织(18.28±11.33)和正常组织(10.80±10.20)(P均＜0.001),癌旁组织明显高于正常组织(P＜0.05);正常结肠组织中Syndecan-1 mRNA表达水平(61.21±12.96)显著高于癌旁组织(14.35±11.06)和结肠癌组织(10.12±8.58)(P均＜0.001),癌旁组织明显高于结肠癌组织(P＜0.05).Syndecan-1 mRNA的表达减弱和HPA-1的表达增强与肿瘤的分化程度、浸润深度、淋巴结转移、远处转移及TNM分期显著相关(P均＜0.05).Spearman等级相关分析证明了Syndecan-1 tuRNA和HPA-1的表达呈明显负相关(r=-0.405,P＜0.05).结论:Syndecan-1 mRNA在结肠癌组织中呈低表达,HPA-1 mRNA在结肠癌组织中呈高表达,且Syndecan-1 mRNA的表达减弱与HPA-1的表达增强可促进结肠癌的侵袭转移.联合检测Syndecan-1 mRNA及HPA-1mRNA对于指导结肠癌临庆治疗及判断预后有重要价值.
배경여목적:침습전이시도치결장암사망솔증가적주요원인,여하조단종류적침습전이성위목전연구적열점.본연구지재검측Syndecan-1기인화HPA-1기인재결장암조직중적표체수평,탐토이자여결장암침습전이급예후적관계.방법:용실시형광정량역전록취합매련반응(RT-PCR)분별검측49례결장암、49례배대적암방조직(거암2cm)화49례배대원리결장암적수술절연정상조직(거암5cm이상)적Syndecan-1화HPA-1기인적표체수평,분석이자여결장암림상병리특정적관계.결과:결장암중HPA-1mRNA적표체수평(40.56±11.75)현저고우암방조직(18.28±11.33)화정상조직(10.80±10.20)(P균＜0.001),암방조직명현고우정상조직(P＜0.05);정상결장조직중Syndecan-1 mRNA표체수평(61.21±12.96)현저고우암방조직(14.35±11.06)화결장암조직(10.12±8.58)(P균＜0.001),암방조직명현고우결장암조직(P＜0.05).Syndecan-1 mRNA적표체감약화HPA-1적표체증강여종류적분화정도、침윤심도、림파결전이、원처전이급TNM분기현저상관(P균＜0.05).Spearman등급상관분석증명료Syndecan-1 tuRNA화HPA-1적표체정명현부상관(r=-0.405,P＜0.05).결론:Syndecan-1 mRNA재결장암조직중정저표체,HPA-1 mRNA재결장암조직중정고표체,차Syndecan-1 mRNA적표체감약여HPA-1적표체증강가촉진결장암적침습전이.연합검측Syndecan-1 mRNA급HPA-1mRNA대우지도결장암림경치료급판단예후유중요개치.
Background and Objective:Invasion and metastasis are the most common causes of mortality for patients with colorectaI neoplasms,and blocking invasion and metastasis in a timely fashion has become a hot research focus.We investigated the expression of the messenger RNA (mRNA)of Syndecan-1 and HPA-1 in colorecta J cancer,and their correlation with invasion and metastasis.Methods:Real-time fluorescent quantitative reverse transcription polymerase chain reaction(RT-PCR)was used to detect the expression of Syndecan-1 and HPA-1 in specimens from 49 patients with colorectal cancer.49 paired adjacent colorectal neoplasms(2 cm from the carcinoma),and 49 surgical margins of paired normal colorectal mucosa tissue(5 cm from the carcinoma),to analyze their correlation with clinicopathologic characteristics of colorectal neoplasm.Results:The expression of HPA-1 mRNA was significantly higher in colorectal cancer (40.56±11.75)than that in the paired adjacent colorectal neoplasms(18.28±11.33)and normal colorectal mucosa tissue(10.80±1020)(all P＜0.001).The expression of HPA-1 mRNA was significantly higher in paired adjacent colorectal neoplasms than that in normal colorectal mucosa(P＜0.05).The expression of Syndecan-1 mRNA was significantly higher in normal colorectal mucosa(61.21±12.96)than in the paired adjacent mucosa (14.35±11.06)or colorectaI cancer(10.12±8.58)(all P＜0.001).The expression of Syndecan-1 mRNA was significantly higher in the paired adjacent mucosa than that in colorectal cancer(P＜0.05).The decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 were closely associated with the degree of differentiation,the depth of infiltration,lymph node metastasis,vessel metastasis,and TNM staging of colorectal cancer(all P＜0.05).Spearman rank correlation analysis demonstrated a significant correlation between Syndecan-1 and HPA-1(r=-0.405,P＜0.05).Conclusions:The expression of Syndecan-1 mRNA was significantly highest in normal colorectal mucosa and the expression of HPA-1 mRNA was significantly highest in colorectal cancer.At the same time,the decreased expression of Syndecan-1 mRNA and the increased expression of HPA-1 mRNA can promote the invasion and metastasis of colorectal cancer.The determination of Syndecan-1 and HPA-1 may be of value in the treatment as well as in the prognosis of patients with colorectal cancer.