CAJ | 학술논문

目的研究幼年大鼠反复惊厥后海马组织caspase-3、FasL表达的变化.方法 72只20日龄健康Sprague-Dawley(SD)大鼠随机分为2组:正常对照组、惊厥组.通过三氟乙醚反复吸入(1次/d,连续6 d),制作幼年大鼠惊厥动物模型.用免疫组化(SP)法检测各组动物反复惊厥结束后1、3、7 d海马组织中caspase-3和FasL蛋白的表达,RT-PCR方法检测各时相点海马组织中caspase-3 mRNA的表达.结果反复惊厥结束后1、3、7 d海马组织caspase-3、FasL的表达和对照组比较,差异有统计学意义(P<0.01).结论 caspase-3、FasL参与了幼年期惊厥性脑损伤的病理过程.
목적 연구유년대서반복량궐후해마조직caspase-3、FasL표체적변화.방법 72지20일령건강Sprague-Dawley(SD)대서수궤분위2조:정상대조조、량궐조.통과삼불을미반복흡입(1차/d,련속6 d),제작유년대서량궐동물모형.용면역조화(SP)법검측각조동물반복량궐결속후1、3、7 d해마조직중caspase-3화FasL단백적표체,RT-PCR방법검측각시상점해마조직중caspase-3 mRNA적표체.결과 반복량궐결속후1、3、7 d해마조직caspase-3、FasL적표체화대조조비교,차이유통계학의의(P<0.01).결론 caspase-3、FasL삼여료유년기량궐성뇌손상적병리과정.
Objective To investigate the expression of caspase-3 and FasL in the hippocampus of the infantile rats with recurrent sei-zures. Methods 72 of 20-day-old Sprague-Dawley rats were randomly divided into two groups, control group and seizure group. Seizures in rats were induced by inhalant flurothyl daily in six consecutive days. Brain tissue was sampled at different time points (the 1st day, 3rd day and 7th day) after last seizure. The expressions of caspase-3 and FasL proteins in the hippocampus were detected by immunohistochemistry, and the expression of caspase-3 mRNA was measured by reverse transcription- polymerase chain reaction (RT-PCR). Results The caspase-3 protein, FasL protein and caspnse-3 mRNA levels were obviously increased at the 1st, 3rd and 7th day after recurrent seizure in the hippocampus of the rat(P<0.01). Conclusions Caspase-3 and FasL are participated in the infantile brain injury after recurrent seizures.