中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2010年
9期
1088-1090
,共3页
依托咪酯%缺血预处理%细胞凋亡%肾上腺皮质
依託咪酯%缺血預處理%細胞凋亡%腎上腺皮質
의탁미지%결혈예처리%세포조망%신상선피질
Etomidate%Ischemic preconditioning%Apoptosis%Adrenal cortex
目的 探讨依托咪酯对猪肾上腺皮质细胞的毒性作用及依托咪酯预处理对其的影响.方法 实验分两部分,第一部分为细胞毒性实验,猪肾上腺皮质细胞随机分为8组,每组3皿,对照组加入0.5%二甲基亚砜(DMSO),其他7组分别加入50、100、200、300、400、500、1000 μmol/L依托咪酯,每组分别作用6、12、24 h,进行下述指标检测.第二部分为预处理实验,猪肾上腺皮质细胞随机分为3组,每组3皿,对照组加入0.5%DMSO,损伤组加入325 μmol/L依托咪酯作用24 h,预处理组先加入0.6 μmol/L依托咪酯作用1 h,洗脱后在培养基中孵育4 h,再加入325 μmol/L依托咪酯作用24 h.采用CCK-8法检测细胞活力,计算24 h时依托咪酯半数抑制浓度(IC50),采用流式细胞仪检测细胞凋亡情况.结果 依托咪酯可抑制猪肾上腺皮质细胞活力,诱导细胞凋亡,呈浓度和时间依赖性,24 h时IC50为325μmol/L.0.6 μmol/L依托咪酯预处理1 h可抑制325μmol/L依托咪酯诱发的细胞凋亡具有抑制作用.结论 依托咪酯通过诱发猪肾上腺皮质细胞凋亡对其功能产生抑制作用;依托咪酯预处理可减轻药物自身引起的这种抑制作用.
目的 探討依託咪酯對豬腎上腺皮質細胞的毒性作用及依託咪酯預處理對其的影響.方法 實驗分兩部分,第一部分為細胞毒性實驗,豬腎上腺皮質細胞隨機分為8組,每組3皿,對照組加入0.5%二甲基亞砜(DMSO),其他7組分彆加入50、100、200、300、400、500、1000 μmol/L依託咪酯,每組分彆作用6、12、24 h,進行下述指標檢測.第二部分為預處理實驗,豬腎上腺皮質細胞隨機分為3組,每組3皿,對照組加入0.5%DMSO,損傷組加入325 μmol/L依託咪酯作用24 h,預處理組先加入0.6 μmol/L依託咪酯作用1 h,洗脫後在培養基中孵育4 h,再加入325 μmol/L依託咪酯作用24 h.採用CCK-8法檢測細胞活力,計算24 h時依託咪酯半數抑製濃度(IC50),採用流式細胞儀檢測細胞凋亡情況.結果 依託咪酯可抑製豬腎上腺皮質細胞活力,誘導細胞凋亡,呈濃度和時間依賴性,24 h時IC50為325μmol/L.0.6 μmol/L依託咪酯預處理1 h可抑製325μmol/L依託咪酯誘髮的細胞凋亡具有抑製作用.結論 依託咪酯通過誘髮豬腎上腺皮質細胞凋亡對其功能產生抑製作用;依託咪酯預處理可減輕藥物自身引起的這種抑製作用.
목적 탐토의탁미지대저신상선피질세포적독성작용급의탁미지예처리대기적영향.방법 실험분량부분,제일부분위세포독성실험,저신상선피질세포수궤분위8조,매조3명,대조조가입0.5%이갑기아풍(DMSO),기타7조분별가입50、100、200、300、400、500、1000 μmol/L의탁미지,매조분별작용6、12、24 h,진행하술지표검측.제이부분위예처리실험,저신상선피질세포수궤분위3조,매조3명,대조조가입0.5%DMSO,손상조가입325 μmol/L의탁미지작용24 h,예처리조선가입0.6 μmol/L의탁미지작용1 h,세탈후재배양기중부육4 h,재가입325 μmol/L의탁미지작용24 h.채용CCK-8법검측세포활력,계산24 h시의탁미지반수억제농도(IC50),채용류식세포의검측세포조망정황.결과 의탁미지가억제저신상선피질세포활력,유도세포조망,정농도화시간의뢰성,24 h시IC50위325μmol/L.0.6 μmol/L의탁미지예처리1 h가억제325μmol/L의탁미지유발적세포조망구유억제작용.결론 의탁미지통과유발저신상선피질세포조망대기공능산생억제작용;의탁미지예처리가감경약물자신인기적저충억제작용.
Objective To investigate the effect of etomidate on porcine adrenal cortical cells and the influence of preconditioning with small dose etomidate. Methods Porcine adrenal cortical cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 15% fetal bovine serum at 37℃ in 5% CO2 incubator for 24 h. The concentration was 2 × 106/ml. The experiment was performed in 2 parts. In part Ⅰ the cells were exposed to 50, 100, 200, 300, 400, 500 and 1000 mol/L etomidate respectively and incubated for 6, 12and 24 h, while in control group, the cells were exposed to 0.5% dimethylsulfoxide (DMSO). Cell viability was measured using CCK-8 assay and apoptosis by flow cytometry, and the 50% inhibitory concentration (IC50) of etomidate was calculated at 24 h of incubation. In part Ⅱ the cells were exposed to 0.6 μmol/L etomidate for 1 h and were allowed to recover for 4 h after etomidate washout, then etomidate 325 μmol/L was added and the cells were incubated for 24 h. Cell viability and apoptosis were assessed and the IC50 of etomidate was calculated as in part Ⅰ .Results Etomidate inhibited viability of porcine adrenal cortical cells and induced apoptosis in a dose- and time-dependent manner. The IC50 of etomidate at 24 h of incubation was 325 μmol/L. Preconditioning with0.6 μmol/L etomidate for 1 h attenuated the apoptosis induced by etomidate 325 μmol/L. Conclusion Etomidate can inhibit cell viability and induce apoptosis in a dose- and time-dependent manner. Preconditioning with small dose etomidate has protective effect.
