中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2012年
9期
1286-1287
,共2页
黄小琪%陈晶%林英辉%陈松林%李生%潘元平
黃小琪%陳晶%林英輝%陳鬆林%李生%潘元平
황소기%진정%림영휘%진송림%리생%반원평
结核性胸膜炎%白细胞介素-17%Th17细胞%胸腔积液
結覈性胸膜炎%白細胞介素-17%Th17細胞%胸腔積液
결핵성흉막염%백세포개소-17%Th17세포%흉강적액
tuberculous pleural elfusion%IL-17%Th17 cell%Pleural effusion
目的 探讨结核性胸膜炎外周血和胸水中Th17细胞和白细胞介素-17(IL-17)的表达水平及临床意义.方法 初治结核性胸膜炎(结核组)患者30例和非结核性胸膜炎(胸膜炎组)20例,另选择健康体检人员(对照组)20例.采用流式细胞术和双抗体夹心免疫酶标法(ELISA)测定血清及胸水中Th17细胞的百分率及IL-17的含量.结果 结核组外周血中Th17细胞的百分比和IL-17的含量显著高于对照组和胸膜炎组(P＜0.05).结核组胸水中Th17细胞的百分比和IL-17的含量明显高于胸膜炎组(P＜0.05).结核组患者在规则治疗1、3、7、14 d时,外周血和胸水中Th17细胞的百分比和IL-17的含量均逐渐下降,外周血中TH17细胞的百分比于治疗1d时显著低于入院时水平(P＜0.05),胸水中Th17细胞的百分比于治疗3d时显著低于入院时水平(P＜0.05),外周血中IL-17的含量于治疗3d时显著低于人院时水平(P＜0.05),胸水中IL-17的含量于治疗7d时显著低于入院时水平(P＜0.05).结论 Th17/IL-17参与了结核性胸膜炎的发病机制,且与其病程发展及治疗效果密切相关.
目的 探討結覈性胸膜炎外週血和胸水中Th17細胞和白細胞介素-17(IL-17)的錶達水平及臨床意義.方法 初治結覈性胸膜炎(結覈組)患者30例和非結覈性胸膜炎(胸膜炎組)20例,另選擇健康體檢人員(對照組)20例.採用流式細胞術和雙抗體夾心免疫酶標法(ELISA)測定血清及胸水中Th17細胞的百分率及IL-17的含量.結果 結覈組外週血中Th17細胞的百分比和IL-17的含量顯著高于對照組和胸膜炎組(P＜0.05).結覈組胸水中Th17細胞的百分比和IL-17的含量明顯高于胸膜炎組(P＜0.05).結覈組患者在規則治療1、3、7、14 d時,外週血和胸水中Th17細胞的百分比和IL-17的含量均逐漸下降,外週血中TH17細胞的百分比于治療1d時顯著低于入院時水平(P＜0.05),胸水中Th17細胞的百分比于治療3d時顯著低于入院時水平(P＜0.05),外週血中IL-17的含量于治療3d時顯著低于人院時水平(P＜0.05),胸水中IL-17的含量于治療7d時顯著低于入院時水平(P＜0.05).結論 Th17/IL-17參與瞭結覈性胸膜炎的髮病機製,且與其病程髮展及治療效果密切相關.
목적 탐토결핵성흉막염외주혈화흉수중Th17세포화백세포개소-17(IL-17)적표체수평급림상의의.방법 초치결핵성흉막염(결핵조)환자30례화비결핵성흉막염(흉막염조)20례,령선택건강체검인원(대조조)20례.채용류식세포술화쌍항체협심면역매표법(ELISA)측정혈청급흉수중Th17세포적백분솔급IL-17적함량.결과 결핵조외주혈중Th17세포적백분비화IL-17적함량현저고우대조조화흉막염조(P＜0.05).결핵조흉수중Th17세포적백분비화IL-17적함량명현고우흉막염조(P＜0.05).결핵조환자재규칙치료1、3、7、14 d시,외주혈화흉수중Th17세포적백분비화IL-17적함량균축점하강,외주혈중TH17세포적백분비우치료1d시현저저우입원시수평(P＜0.05),흉수중Th17세포적백분비우치료3d시현저저우입원시수평(P＜0.05),외주혈중IL-17적함량우치료3d시현저저우인원시수평(P＜0.05),흉수중IL-17적함량우치료7d시현저저우입원시수평(P＜0.05).결론 Th17/IL-17삼여료결핵성흉막염적발병궤제,차여기병정발전급치료효과밀절상관.
Objective To investigate the blood and chest fluid level of Th17 cell and IL-17 in patients with tuberculous pleural effusion and its pathological role.Methods Flow cytometry and enzyme linked immunosorbent assay (ELISA) were used to measure the blood and chest fluid level of Th17 cell and IL-17 from 30 patients with tuberculous pleural effusion,20 patients without tuberculous pleural effusion,and 20 healthy persons.Results The blood level of Th17 cell and IL-17 wwere higher in tuberculous pleural effusion than in the other two groups(P ＜0.05).The chest fluid level of Th17 cell and IL-17 in patients with tuberculous pleural effusion were significantly higher than those in patients without tuberculous pleural effusion (P ＜ 0.05 ).The chest fluid level of Th17 cell and IL-17 in patients with and without tuberculous pleural effusion were significantly higher than that of the blood serum level.After treatment for 1,3,7 and 14 days,tbe blood serum and chest fluid level of Th17 cell and IL-17 were obviously lower.( P ＜ 0.01 ).After treatment for 1 day,the blood level of Th17 cell was obviously lower than before treatment( P ＜ 0.01 ).After treatment for 3 days,the chest fluid level of Th17 cell was obviously lower than before treatment( P ＜0.01 ).After treatment for 3 days,the blood serum level of IL-17 was obviously lower than before treatment (P ＜ 0.01 ).After treatment for 7 days,the chest fluid level of IL-17 was obviously lower than before treatment ( P ＜0.01 ).Conclusion Th17 cell and IL-17 might play an important role in the pathogenesis of tuberculous pleural effusion and they were correlated with disease progression and the therapeutic effect.
