中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
9期
1206-1207
,共2页
呼吸机相关肺损伤%HSP70%NF-κB
呼吸機相關肺損傷%HSP70%NF-κB
호흡궤상관폐손상%HSP70%NF-κB
Ventilator-induced lung injury%Heat shock protein 70%NF-kappa B
目的 探讨呼吸机所致肺损伤(VILI)炎症反应中热休克蛋白70(HSP70)和核因子-κB(NF-κB)表达的意义.方法 健康SD大鼠30只,实施麻醉和气管切开术后分别接受不同潮气量(VT)的通气(通气时间均为4 h).随机分为以下3组:对照组(A组);常规通气组(B组,VT=10ml/kg);损伤通气组(C组,VT=40ml/kg).4 h后放血处死动物,测定肺湿/干比重、髓过氧化物酶(MPO)活性及肺泡灌洗液(BALF)中总蛋白含量,光镜下行白细胞(WBC)计数;Western blot检测肺组织HSP70及NF-κB的表达.结果 在大潮气量机械通气4 h后,BALF中总蛋白含量、肺湿干比、WBC计数和MPO水平(3.89±0.31、5.83±0.42、6.10±0.80、7.31±1.21)均明显更高(P＜0.05);肺组织中HSP70、NF-κB表达水平(1.36±0.13、0.41±0.07)均显著增加,且两者呈负相关.结论 HSP70可通过抑制NF-κB的活性,保护肺组织避免VILI.
目的 探討呼吸機所緻肺損傷(VILI)炎癥反應中熱休剋蛋白70(HSP70)和覈因子-κB(NF-κB)錶達的意義.方法 健康SD大鼠30隻,實施痳醉和氣管切開術後分彆接受不同潮氣量(VT)的通氣(通氣時間均為4 h).隨機分為以下3組:對照組(A組);常規通氣組(B組,VT=10ml/kg);損傷通氣組(C組,VT=40ml/kg).4 h後放血處死動物,測定肺濕/榦比重、髓過氧化物酶(MPO)活性及肺泡灌洗液(BALF)中總蛋白含量,光鏡下行白細胞(WBC)計數;Western blot檢測肺組織HSP70及NF-κB的錶達.結果 在大潮氣量機械通氣4 h後,BALF中總蛋白含量、肺濕榦比、WBC計數和MPO水平(3.89±0.31、5.83±0.42、6.10±0.80、7.31±1.21)均明顯更高(P＜0.05);肺組織中HSP70、NF-κB錶達水平(1.36±0.13、0.41±0.07)均顯著增加,且兩者呈負相關.結論 HSP70可通過抑製NF-κB的活性,保護肺組織避免VILI.
목적 탐토호흡궤소치폐손상(VILI)염증반응중열휴극단백70(HSP70)화핵인자-κB(NF-κB)표체적의의.방법 건강SD대서30지,실시마취화기관절개술후분별접수불동조기량(VT)적통기(통기시간균위4 h).수궤분위이하3조:대조조(A조);상규통기조(B조,VT=10ml/kg);손상통기조(C조,VT=40ml/kg).4 h후방혈처사동물,측정폐습/간비중、수과양화물매(MPO)활성급폐포관세액(BALF)중총단백함량,광경하행백세포(WBC)계수;Western blot검측폐조직HSP70급NF-κB적표체.결과 재대조기량궤계통기4 h후,BALF중총단백함량、폐습간비、WBC계수화MPO수평(3.89±0.31、5.83±0.42、6.10±0.80、7.31±1.21)균명현경고(P＜0.05);폐조직중HSP70、NF-κB표체수평(1.36±0.13、0.41±0.07)균현저증가,차량자정부상관.결론 HSP70가통과억제NF-κB적활성,보호폐조직피면VILI.
Objective To investigate the expression and significance of heat shock protein 70 (HSP70) and nuclear factor-κB (NF-κB) in a rat model of ventilator-induced lung injury (VILI). Methods Thirty healthy SD rats were randomly divided into three groups (each 10 rats): control group (group A, no ventilation), conventional ventilation group (group B, VT = 10 ml/kg), lung injury group (group C, VT =40 ml/kg). After anesthesia and tracheotomy were performed, rats received ventilation with different volumes for 4 h. The rats were killed by exsanguinations. The lung wet/dry weight, myeloperoxidase (MPO) activity and total protein level and counts of white blood cells (WBC) in broncho-alveolar lavage fluid (BALF) were tested. The expression of HSP70 and NF-κB was detected by using Western blotting.Results After injurious ventilation for 4 h, the level of lung wet/dry weight, MPO activity and total protein level and counts of WBC were 5.83 ± 0.42, 7.31 ± 1.21, 3.89 ± 0.31, 6. 10 ± 0. 80, respectively.The average values of HSP70 and NF-κB proteins were 1.36 ±0.13, and 0. 41 ±0.07, respectively, and there was a negative correlation between them. Conclusion Tidal volume mechanical ventilation can induce lung tissue damage and HSP70 can inhibit the activity of NF-κB to protect lung tissue from VILI in rats.
