中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2006年
1期
33-37
,共5页
梅元武%魏桂荣%张敏%董继华%袁光雷%陈运平
梅元武%魏桂榮%張敏%董繼華%袁光雷%陳運平
매원무%위계영%장민%동계화%원광뢰%진운평
脑炎,病毒性%肠道病毒属%逆转录聚合酶链反应
腦炎,病毒性%腸道病毒屬%逆轉錄聚閤酶鏈反應
뇌염,병독성%장도병독속%역전록취합매련반응
Encephalitis,viral%Enterovirus%Reverse transcription polymerase chain reaction
目的探讨对病毒性脑炎患者的脑脊液(CSF)进行肠道病毒(EV)逆转录-聚合酶链式反应(RT-PCR)检测的临床诊断和治疗意义.方法用1对针对EV多数血清型的特异性通用引物组建的逆转录-聚合酶链式反应对43例临床诊断为病毒性脑炎患者的CSF进行临床检测,同时观察这些患者的临床症状、体征、脑脊液生化常规及病毒其他病原体检查,头颅影像学和脑电图检查,用t检验进行统计学分析.结果CSF肠道病毒逆转录-聚合酶链式反应(EV RT-PCR)阳性者18人,占41.9%,临床症状、病情轻重与阴性者无明显差异,CSF蛋白和细胞数较高,可同时合并血中其他病毒(单纯疱疹病毒、巨细胞病毒)抗体阳性.结论EV引起的病毒性脑炎发病率高,并可合并其他病毒感染,EV RT-PCR与血清学检查结果存在不一致性,EV RT-PCR检查结果可指导临床诊断和针对性用药.
目的探討對病毒性腦炎患者的腦脊液(CSF)進行腸道病毒(EV)逆轉錄-聚閤酶鏈式反應(RT-PCR)檢測的臨床診斷和治療意義.方法用1對針對EV多數血清型的特異性通用引物組建的逆轉錄-聚閤酶鏈式反應對43例臨床診斷為病毒性腦炎患者的CSF進行臨床檢測,同時觀察這些患者的臨床癥狀、體徵、腦脊液生化常規及病毒其他病原體檢查,頭顱影像學和腦電圖檢查,用t檢驗進行統計學分析.結果CSF腸道病毒逆轉錄-聚閤酶鏈式反應(EV RT-PCR)暘性者18人,佔41.9%,臨床癥狀、病情輕重與陰性者無明顯差異,CSF蛋白和細胞數較高,可同時閤併血中其他病毒(單純皰疹病毒、巨細胞病毒)抗體暘性.結論EV引起的病毒性腦炎髮病率高,併可閤併其他病毒感染,EV RT-PCR與血清學檢查結果存在不一緻性,EV RT-PCR檢查結果可指導臨床診斷和針對性用藥.
목적탐토대병독성뇌염환자적뇌척액(CSF)진행장도병독(EV)역전록-취합매련식반응(RT-PCR)검측적림상진단화치료의의.방법용1대침대EV다수혈청형적특이성통용인물조건적역전록-취합매련식반응대43례림상진단위병독성뇌염환자적CSF진행림상검측,동시관찰저사환자적림상증상、체정、뇌척액생화상규급병독기타병원체검사,두로영상학화뇌전도검사,용t검험진행통계학분석.결과CSF장도병독역전록-취합매련식반응(EV RT-PCR)양성자18인,점41.9%,림상증상、병정경중여음성자무명현차이,CSF단백화세포수교고,가동시합병혈중기타병독(단순포진병독、거세포병독)항체양성.결론EV인기적병독성뇌염발병솔고,병가합병기타병독감염,EV RT-PCR여혈청학검사결과존재불일치성,EV RT-PCR검사결과가지도림상진단화침대성용약.
Objective To understand the effects of mRNA assay for enterovirus in cerebrospinal fluid (CSF) of patients by using reverse transcription polymerase chain reaction (RT-PCR) on clinical diagnosis and therapy of viral encephalitis. Methods RT-PCR with one pair of picornaviridae-specific primer (PSP) against the conserved 5'noncoding region of enterovirus (EV) was employed to investigate enterovirus RNA in CSF of 43 patients who were diagnosed as viral encephalitis by their clinical features.The patients' symptoms, abnormal signs and laboratory evaluation including routine examinations of CSF,CSF biochemistry, antibodies to other virus in CSF, cranial computed tomography (CT), cranial magnetic resonance imaging (MRI) and electroencephalogram (EEG) were observed and statistically analyzed by Student's tests. Results By the CSF EV RT-PCR, 18 out of 43 patients, most of them male, were tested to be EV positive (41.9%), and their symptoms and signs were not significantly different from that of EV negative. The albumen and cell content in CSF of the patients of EV positive was higher than patients of negative. Sometimes antibodies to other virus could be found in serum of EV positive patients.Conclusion Viral encephalitis owing to enterovirus has higher morbidity and sometimes goes with other virus infection. The results of EV RT-PCR are not always consistent with that of serum assay. The result of EV RT-PCR could be used to guide clinical diagnosis and treatment.