CAJ | 학술논문

目的研究环孢素A(CsA)对大鼠脑损伤后血清中髓鞘碱性蛋白抗体(Anti-MBP)及继发性脑干脱髓鞘病变程度的影响.方法 SD大鼠77只按随机数字表法分为空白对照组(n=7)、模型组(n=35)、CsA组(n=35).后2组大鼠制作闭合性重型颅脑损伤模型,术后模型组大鼠持续腹腔注射0.9%生理盐水[5 mg/(kg·d)],CsA组大鼠腹腔注射5 mg/mL CsA[5 mg/(kg·d)],于不同时间点(伤后1、4、10、20、30d)处死大鼠后采用ELLSA法检测血清MBP和Anti-MBP含量,Marchi锇酸染色法检测脑干脱髓鞘病变程度.结果与空白对照组比较.模型组大鼠伤后血清MBP、Anti-MBP含量升高,差异有统计学意义(P<0.05);与模型组同一时间点比较,CsA组大鼠血清MBP含量差异无统计学意义(P>0.05),而伤后1d开始Anti-MBP含量降低、伤后4 d开始变性髓鞘数量降低.差异有统计学意义(P<0.05);Pearson相关分析显示CsA组与模型组大鼠血清Anti-MBP含量与脑干变性髓鞘的数量呈正相关关系(r=0.959,P=0.000).结论 CsA可以干预脑组织损伤后大鼠的免疫系统,在一定程度上可以减少Anti-MBP的产生、减轻大鼠继发性脑干脱髓鞘病变的程度.
목적 연구배포소A(CsA)대대서뇌손상후혈청중수초감성단백항체(Anti-MBP)급계발성뇌간탈수초병변정도적영향.방법 SD대서77지안수궤수자표법분위공백대조조(n=7)、모형조(n=35)、CsA조(n=35).후2조대서제작폐합성중형로뇌손상모형,술후모형조대서지속복강주사0.9%생리염수[5 mg/(kg·d)],CsA조대서복강주사5 mg/mL CsA[5 mg/(kg·d)],우불동시간점(상후1、4、10、20、30d)처사대서후채용ELLSA법검측혈청MBP화Anti-MBP함량,Marchi철산염색법검측뇌간탈수초병변정도.결과 여공백대조조비교.모형조대서상후혈청MBP、Anti-MBP함량승고,차이유통계학의의(P<0.05);여모형조동일시간점비교,CsA조대서혈청MBP함량차이무통계학의의(P>0.05),이상후1d개시Anti-MBP함량강저、상후4 d개시변성수초수량강저.차이유통계학의의(P<0.05);Pearson상관분석현시CsA조여모형조대서혈청Anti-MBP함량여뇌간변성수초적수량정정상관관계(r=0.959,P=0.000).결론 CsA가이간예뇌조직손상후대서적면역계통,재일정정도상가이감소Anti-MBP적산생、감경대서계발성뇌간탈수초병변적정도.
Objective To investigate the effect of cyclosporin A(CsA)on the level of anti-myelin basic protein(anti-MBP)in the serum and myelinoclasis in rat models after brain trauma.Methods Seventy-seven SD rats were randomized into blank control group(n=7),model group(n=35)and CsA-treated group(n=35).The 0.9% normal saline(5 mg/kg/d)was intraperitoneally injected into the model group and 5 mg/mL CsA(5 mg/kg/d)was injected into the CsA-treated group.Different time points(1,4,10,20 and 30 d)after the brain trauma,the MBP content and anti-MBP titer in the serum were measured using enzyme-linked immunosorbent assay(ELISA)and the degree of myelinoclasis in the brain stem slices was assessed with Marchi's method.Results As compared with that in the blank control group,the MBP and Anti-MBP contents in the model group were significantly increased(P<0.05):anti-MBP in the serum and the degree of myelinoclasis in the brain stem were all significantly increased in the 4th d,both reaching their highest levels on 10~(th) d.No significant differences of the MBP content in the serum between the CsA-treated group and the model group were found the same time points after the injury(P>0.05).However,lower level of anti-MBP in the serum 1 d after the injury and degree of myelinoclasis in the brain stem 4 daRer the injury in the CsA-treated group were observed as compared with those in the model group(P<0.05).Statistical analysis showed an intimate positive correlation between anti-MBP titer in serum and the degree of rnyelinoclasis(r=0.959,P=0.000).Conclusion CsA can affect the immune system to decrease the degree of myelinoclasis in the brain stem through decreasing the level of anti-MBP in the serum of rat models after brain trauma.