中国药学(英文版)
中國藥學(英文版)
중국약학(영문판)
JOURNAL OF CHINESE PHARMACEUTICAL SCIENCES
2004年
2期
103-105
,共3页
缪宇平%陈逸民%周宏农%闻韧
繆宇平%陳逸民%週宏農%聞韌
무우평%진일민%주굉농%문인
微小亚历山大藻%膝沟藻毒素%胶滤层析%离子交换层析
微小亞歷山大藻%膝溝藻毒素%膠濾層析%離子交換層析
미소아력산대조%슬구조독소%효려층석%리자교환층석
Alexandrium minutum Halim%gonyautoxins%gel filtration chromatography%ion exchange chromatography
目的从两株微小亚历山大藻(Alexandrium minutum Halim)中分离纯化膝沟藻毒素(Gonyautoxins).方法用胶滤层析、离子交换层析等方法从人工培养的两株微小亚历山大藻酸酒精萃取物中分离纯化膝沟藻毒素,再用HPLC法对毒素进行定性定量测定.结果从两株藻株培养液中均分离得到膝沟藻毒素GTX-4,GTX-1,GTX-3,GTX-2,其中Amtk4藻株的毒素含量为Amtk2的近10倍.结论微小亚历山大藻Amtk4藻株适于制备膝沟藻毒素.
目的從兩株微小亞歷山大藻(Alexandrium minutum Halim)中分離純化膝溝藻毒素(Gonyautoxins).方法用膠濾層析、離子交換層析等方法從人工培養的兩株微小亞歷山大藻痠酒精萃取物中分離純化膝溝藻毒素,再用HPLC法對毒素進行定性定量測定.結果從兩株藻株培養液中均分離得到膝溝藻毒素GTX-4,GTX-1,GTX-3,GTX-2,其中Amtk4藻株的毒素含量為Amtk2的近10倍.結論微小亞歷山大藻Amtk4藻株適于製備膝溝藻毒素.
목적종량주미소아력산대조(Alexandrium minutum Halim)중분리순화슬구조독소(Gonyautoxins).방법용효려층석、리자교환층석등방법종인공배양적량주미소아력산대조산주정췌취물중분리순화슬구조독소,재용HPLC법대독소진행정성정량측정.결과종량주조주배양액중균분리득도슬구조독소GTX-4,GTX-1,GTX-3,GTX-2,기중Amtk4조주적독소함량위Amtk2적근10배.결론미소아력산대조Amtk4조주괄우제비슬구조독소.
Aim To study isolation and purification of Gonyautoxins from two different strains of Alexandrium minutum Halim. Methods Ethanol extracts of the two culture strains of Alexandrium minutum Halim were fractionated by gel filtra- tion and ion exchange chromatography to obtain gonyautoxins. The toxin profiles were tested by HPLC method. Results Both strains contain gonyautoxin-1, gonyautoxin-2, gonyautoxin-3 and gonyautoxin-4. The total gonyautoxin content of Amtk4 strain is almost 10 times higher than that of Amtk2 strain. Conchusion Alexandrium minutum Halim Amtk4 strain is suitable for the preparation of Gonyautoxins.