生命科学研究
生命科學研究
생명과학연구
LIFE SCIENCE RESEARCH
2009年
5期
443-447
,共5页
刘梅冬%龚环宇%谭斯品%张智炜%陈广文%肖献忠
劉梅鼕%龔環宇%譚斯品%張智煒%陳廣文%肖獻忠
류매동%공배우%담사품%장지위%진엄문%초헌충
肺癌%甲基化%WIF-1基因%巢式甲基化特异性聚合酶链反应
肺癌%甲基化%WIF-1基因%巢式甲基化特異性聚閤酶鏈反應
폐암%갑기화%WIF-1기인%소식갑기화특이성취합매련반응
lung cancer%methylation%WIF-1%nested methylation-specific PCR
为了检测肺癌患者血浆中WIF-1基因启动子区的甲基化状态,收集肺癌患者及健康对照者的血浆标本,采用巢式甲基化特异性PCR(nMSP)法检测WIF-1基因启动子区甲基化状态.并与普通甲基化特异性PCR(MSP)法进行了比较,结果在58例肺癌患者血浆样品中经nMSP法发现20例WIF-1基因启动子的过甲基化,用MSP法只检出10例,,有吸烟史组WIF-1基因的甲基化率高于无吸烟史组(P<0.05).而20例正常对照血浆中都未检测到WIF-1基因启动子的过甲基化;表明利用巢式MSP(nMSP)法检测外周血血浆中WIF-1基因启动子的甲基化,可为非损伤性筛选和早期诊断肺癌提供有价值的信息.
為瞭檢測肺癌患者血漿中WIF-1基因啟動子區的甲基化狀態,收集肺癌患者及健康對照者的血漿標本,採用巢式甲基化特異性PCR(nMSP)法檢測WIF-1基因啟動子區甲基化狀態.併與普通甲基化特異性PCR(MSP)法進行瞭比較,結果在58例肺癌患者血漿樣品中經nMSP法髮現20例WIF-1基因啟動子的過甲基化,用MSP法隻檢齣10例,,有吸煙史組WIF-1基因的甲基化率高于無吸煙史組(P<0.05).而20例正常對照血漿中都未檢測到WIF-1基因啟動子的過甲基化;錶明利用巢式MSP(nMSP)法檢測外週血血漿中WIF-1基因啟動子的甲基化,可為非損傷性篩選和早期診斷肺癌提供有價值的信息.
위료검측폐암환자혈장중WIF-1기인계동자구적갑기화상태,수집폐암환자급건강대조자적혈장표본,채용소식갑기화특이성PCR(nMSP)법검측WIF-1기인계동자구갑기화상태.병여보통갑기화특이성PCR(MSP)법진행료비교,결과재58례폐암환자혈장양품중경nMSP법발현20례WIF-1기인계동자적과갑기화,용MSP법지검출10례,,유흡연사조WIF-1기인적갑기화솔고우무흡연사조(P<0.05).이20례정상대조혈장중도미검측도WIF-1기인계동자적과갑기화;표명이용소식MSP(nMSP)법검측외주혈혈장중WIF-1기인계동자적갑기화,가위비손상성사선화조기진단폐암제공유개치적신식.
To evaluate the methylation status of the promoter region of Wnt inhibitory factor-1 (WIF-1) gene in DNA extracted from plasma of lung cancer patients, nested methylation-specific PCR (nMSP) was used to detect WIF-1 promoter hypermethylation in plasma DNA from 58 cases of lung cancer patients, plasma samples from 20 healthy individuals were used as controls. Compared with methylation-specific PCR (MSP), nMSP was more sensitive in detecting the WIF-1 promoter hypermethylation. Aberrant promoter methylation of the WIF-1 gene was found in 20 out of 58 plasma samples using nMSP, while only 10 cases was detected using MSP. Hypermethylation of WIF-1 gene was more frequent in smoking group. No aberrant promoter methylation was detected in the peripheral plasma of the healthy individuals. The data show that detection of WIF-1 gene promoter hypermethylation in the plasma of lung cancer patients by nMSP can give useful information as well as an effective noninvasive method in screening and early diagnosis of lung cancer patients.