时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2010年
2期
315-317
,共3页
补肾益智汤%老年痴呆模型%超氧化物歧化酶%丙二醛%学习记忆%大鼠
補腎益智湯%老年癡呆模型%超氧化物歧化酶%丙二醛%學習記憶%大鼠
보신익지탕%노년치태모형%초양화물기화매%병이철%학습기억%대서
BushenYizhi decoction%Alzheimer'%sdisease model%SOD%MDA%Learning and memory%Rats
目的 观察补肾益智汤对老年痴呆模型大鼠学习记忆和超氧化物歧化酶(SOD) 、丙二醛( MDA)的影响.方法 颈背部皮下注射D-半乳糖及侧脑室注射Aβ_(25-35)制作老年痴呆大鼠模型, 以低5[g/(kg·d)]、中10[g/(kg·d)]、高20[g/(kg·d)]3个剂量的补肾益智汤灌胃治疗,通过八臂迷宫测试各组大鼠的学习记忆能力,采用比色法测定大鼠血清中SOD活力,MDA含量.结果 高剂量补肾益智汤能显著减少大鼠参考记忆错误(RME)(P<0.05),对工作记忆错误(WME)有降低趋势,但无显著性差异;高剂量补肾益智汤能显著提高大鼠血清中SOD活力 (P<0.01); 中、高剂量补肾益智汤能显著降低大鼠血清中MDA含量 (P<0.01).结论 通过降低自由基水平可能是补肾益智汤治疗老年痴呆模型大鼠的机理之一.
目的 觀察補腎益智湯對老年癡呆模型大鼠學習記憶和超氧化物歧化酶(SOD) 、丙二醛( MDA)的影響.方法 頸揹部皮下註射D-半乳糖及側腦室註射Aβ_(25-35)製作老年癡呆大鼠模型, 以低5[g/(kg·d)]、中10[g/(kg·d)]、高20[g/(kg·d)]3箇劑量的補腎益智湯灌胃治療,通過八臂迷宮測試各組大鼠的學習記憶能力,採用比色法測定大鼠血清中SOD活力,MDA含量.結果 高劑量補腎益智湯能顯著減少大鼠參攷記憶錯誤(RME)(P<0.05),對工作記憶錯誤(WME)有降低趨勢,但無顯著性差異;高劑量補腎益智湯能顯著提高大鼠血清中SOD活力 (P<0.01); 中、高劑量補腎益智湯能顯著降低大鼠血清中MDA含量 (P<0.01).結論 通過降低自由基水平可能是補腎益智湯治療老年癡呆模型大鼠的機理之一.
목적 관찰보신익지탕대노년치태모형대서학습기억화초양화물기화매(SOD) 、병이철( MDA)적영향.방법 경배부피하주사D-반유당급측뇌실주사Aβ_(25-35)제작노년치태대서모형, 이저5[g/(kg·d)]、중10[g/(kg·d)]、고20[g/(kg·d)]3개제량적보신익지탕관위치료,통과팔비미궁측시각조대서적학습기억능력,채용비색법측정대서혈청중SOD활력,MDA함량.결과 고제량보신익지탕능현저감소대서삼고기억착오(RME)(P<0.05),대공작기억착오(WME)유강저추세,단무현저성차이;고제량보신익지탕능현저제고대서혈청중SOD활력 (P<0.01); 중、고제량보신익지탕능현저강저대서혈청중MDA함량 (P<0.01).결론 통과강저자유기수평가능시보신익지탕치료노년치태모형대서적궤리지일.
Objective To investigate the effect of BushenYizhi decoction on learning memory,superoxide dismutase(SOD)and maleic dialdehyde(MDA) in Alzheimer's disease rats. Methods The AD model of mice was established by the injection of Aβ_(25-35) into the lateral cerebral ventricle and was given D-galactose, sc . low, middle, high(5,10,20g/kg·d) doses of BushenYizhi decoction were orally administered.The learning and memory ability of the AD model rats were detected by 8-arm radial maze test. The activity of SOD and the MDA in serum were examined by colorimetric method.Results High dose of BushenYizhi decoction could decrease reference memory error(RME) in AD rats ( P<0.05),reduce working memory error(WME),and improve the activity of SOD (P<0.01). Middle and high doses of BushenYizhi decoction could decrease the content of MDA(P<0.01).Conclusion By reducing the level of free radicals may be the mechanisms in treating Alzheimer's rats with Bushenyizhi decoction.
