CAJ | 학술논문

借助表皮条实验和激光扫描共聚焦显微镜技术,研究了天然和合成生长素IAA、NAA、2,4-D、细胞分裂素ZT、KT、6-BA在光、暗调控气孔运动中的作用及其机制.结果表明,暗中所试生长素、细胞分裂素、NO清除剂cPTIO、Hb、NO合酶抑制剂L-NAME、H_2O_2清除剂AsA、CAT、H_2O_2产生酶NADPH氧化酶抑制剂DPI对气孔开放的效应显著大于光下,暗示内源生长素、细胞分裂素、NO、H_2O_2均参与光/暗调控气孔运动,光下内源生长素、细胞分裂素浓度大于暗中,但暗中内源NO、H_2O_2水平高于光下.另外,所试生长素、细胞分裂素和NO、H_2O_2清除剂及合成抑制剂对光、暗条件下气孔运动效应的一致性还表明,生长素、细胞分裂素可能通过降低保卫细胞内源NO、H_2O_2促进气孔开放,内源NO、H_2O_2检测的结果正好证明了这一推论.
차조표피조실험화격광소묘공취초현미경기술,연구료천연화합성생장소IAA、NAA、2,4-D、세포분렬소ZT、KT、6-BA재광、암조공기공운동중적작용급기궤제.결과표명,암중소시생장소、세포분렬소、NO청제제cPTIO、Hb、NO합매억제제L-NAME、H_2O_2청제제AsA、CAT、H_2O_2산생매NADPH양화매억제제DPI대기공개방적효응현저대우광하,암시내원생장소、세포분렬소、NO、H_2O_2균삼여광/암조공기공운동,광하내원생장소、세포분렬소농도대우암중,단암중내원NO、H_2O_2수평고우광하.령외,소시생장소、세포분렬소화NO、H_2O_2청제제급합성억제제대광、암조건하기공운동효응적일치성환표명,생장소、세포분렬소가능통과강저보위세포내원NO、H_2O_2촉진기공개방,내원NO、H_2O_2검측적결과정호증명료저일추론.
The roles and mechanisms of natural and synthetic auxin IAA, NAA, 2,4-D, cytokinin ZT, KT, 6-BA in light/dark-regulated stomatal movement were investigated by means of stomatal bioassay and using laser-scanning congocal microscopy. The results showed that the effects of auxin IAA, NAA, 2,4-D, cytokinin ZT, KT, 6-BA and NO scavenger cPTIO, Hb, NOS inhibitor L-NAME, H_2O_2 scavenger AsA, CAT, NADPH oxidase inhibitor DPI on stomatal opening were more significant in dark than in light, suggesting that endogenous auxin, cytokinin, NO and H_2O_2 mediated light/dark-regulated stomatal movement, and the levels of endogenous auxin and cytokinin were more higher in light than in dark. However, The results indicated that endogenous NO and H_2O_2 were more greater in dark than in light. In addition, the effects of auxin and cytokinin tested on stomatal movement in light or in darkness are accordant with that of NO and H_2O_2 scavengers, synthetic inhibitors, the results imply that auxin and cytokinin probably promoted stomatal opening via decreasing endogenous NO and H_2O_2, which was verified by the monitoring results of endogenous NO, H_2O_2 fluorescence.