CAJ | 학술논문

为构建血管瘤病变型J亚群禽白血病病毒E元件缺失突变体病毒,本研究利用融合PCR方法将E元件缺失,获得含有缺失性突变体病毒前病毒全基因组的重组质粒pSCAU-HN-△E.将该重组质粒纯化后转染DF-1细胞,并连续传代,最终获得缺失病毒rSCAU-HN-△E.该毒株感染的DF-1细胞可被ALV-J特异性单克隆抗体JE9所识别,并且体外增殖性能稳定.该缺失性病毒的获得为E元件功能的研究奠定了基础.
위구건혈관류병변형J아군금백혈병병독E원건결실돌변체병독,본연구이용융합PCR방법장E원건결실,획득함유결실성돌변체병독전병독전기인조적중조질립pSCAU-HN-△E.장해중조질립순화후전염DF-1세포,병련속전대,최종획득결실병독rSCAU-HN-△E.해독주감염적DF-1세포가피ALV-J특이성단극륭항체JE9소식별,병차체외증식성능은정.해결실성병독적획득위E원건공능적연구전정료기출.
The Subgroup J avian leukosis virus (ALV-J) swain SCAU-HN06 isolated from commercial layer hens with spontaneous hemangiomas has a complete "E" element in YUTR. In order to rescue the E element-deleted vires, we constructed the recombinant plasmid pSCAU-HN-△E that contained the whole ALV-J genome but with E element deleted. The plasmid was transfected into DF-1 cells and the rescued virus was identified by indirect fluorescence antibody test with ALV-J specific monoclonal antibody JE9. The recombinant virus was stable in cell culture during nine passages and had a titre up to 10~3.6TCID_(50)/0.2 mL.