天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2010年
2期
281-284
,共4页
何新龙%傅洪拓%龚永生%吴滟%熊贻伟%蒋速飞
何新龍%傅洪拓%龔永生%吳滟%熊貽偉%蔣速飛
하신룡%부홍탁%공영생%오염%웅이위%장속비
丁香酚%日本沼虾%麻醉剂%应激反应
丁香酚%日本沼蝦%痳醉劑%應激反應
정향분%일본소하%마취제%응격반응
eugenol%Macrobrachium nipponense%anaesthetic,%stress response
本实验以丁香酚和日本沼虾为对象,研究了丁香酚对日本沼虾因温度的急剧变化和形态测量所造成的应激反应的抑制效果.结果显示,丁香酚浓度为500 mg/L时,对照组和实验组的存活率开始呈显著下降趋势的温差幅度分别为10和14℃;当温差为18℃时,对照组存活率为0,实验组存活率为0.653±0.033;当温差达到10℃后,实验组存活率明显高于对照组存活率(P<0.05).形态测量结果显示,实验组的断肢率显著低于对照组,而存活率显著高于对照组(P<0.05).研究表明,丁香酚可明显提高日本沼虾存活率,并显著抑制日本沼虾的应激反应.
本實驗以丁香酚和日本沼蝦為對象,研究瞭丁香酚對日本沼蝦因溫度的急劇變化和形態測量所造成的應激反應的抑製效果.結果顯示,丁香酚濃度為500 mg/L時,對照組和實驗組的存活率開始呈顯著下降趨勢的溫差幅度分彆為10和14℃;噹溫差為18℃時,對照組存活率為0,實驗組存活率為0.653±0.033;噹溫差達到10℃後,實驗組存活率明顯高于對照組存活率(P<0.05).形態測量結果顯示,實驗組的斷肢率顯著低于對照組,而存活率顯著高于對照組(P<0.05).研究錶明,丁香酚可明顯提高日本沼蝦存活率,併顯著抑製日本沼蝦的應激反應.
본실험이정향분화일본소하위대상,연구료정향분대일본소하인온도적급극변화화형태측량소조성적응격반응적억제효과.결과현시,정향분농도위500 mg/L시,대조조화실험조적존활솔개시정현저하강추세적온차폭도분별위10화14℃;당온차위18℃시,대조조존활솔위0,실험조존활솔위0.653±0.033;당온차체도10℃후,실험조존활솔명현고우대조조존활솔(P<0.05).형태측량결과현시,실험조적단지솔현저저우대조조,이존활솔현저고우대조조(P<0.05).연구표명,정향분가명현제고일본소하존활솔,병현저억제일본소하적응격반응.
Experiments of eugenol against the stress response of Macrobrachium nipponense,induced by sharp changes in temperature or the measurement of morphology were performed.Experimental result showed that the livability of control group or experiment group began to show a significant declining trend when the temperature difference was 10℃ or 14 ℃ respectively.The livability of control group was 0 while 0.653±0.033 was observed in experiment group when the temperature difference was at 18℃.A higher livability was also obtained in experiment group when compared with control group when tested by t-test at the condition of the temperature difference was up to 10℃(P<0.05).Another result induced by measurement of morphology showed that the leg-injury rate was remarkable lower in test group(0)than in control group,while the livability in test group was significant higher than that of control group(P<0.05).The studies indicated that eugenol can significantly improve the livability and has an excellent efficacy against the stress response to Macrobrachium nipponense.