CAJ | 학술논문

目的观察吲哚美辛对乳腺癌细胞系MCF-7体外迁移能力的影响,探讨可能存在的机制.方法采用Transwell实验方法检测吲哚美辛刺激后MCF-7细胞的迁移能力,运用流式细胞术、Real-timePCR及ELISA方法分别检测吲哚美辛刺激后MCF-7细胞中趋化因子受体(CXCR4)、环氧合酶(COX-2)、表皮生长因子受体( EGFR)和血管内皮生长因子(VEGF)的表达情况.结果吲哚美辛刺激组MCF-7细胞的迁移能力较未刺激组明显降低；吲哚美辛可呈时间-剂量依赖性地抑制MCF-7细胞表面CXCR4的表达,并显著下调细胞中CXCR4、COX-2和EGFR mRNA水平的表达,但对VEGF的产生并无明显的影响.结论MCF-7细胞中CXCR4、COX-2和EGFR表达的显著下调,可能是吲哚美辛抑制MCF-7细胞体外迁移能力的重要机制.
목적 관찰신타미신대유선암세포계MCF-7체외천이능력적영향,탐토가능존재적궤제.방법 채용Transwell실험방법검측신타미신자격후MCF-7세포적천이능력,운용류식세포술、Real-timePCR급ELISA방법분별검측신타미신자격후MCF-7세포중추화인자수체(CXCR4)、배양합매(COX-2)、표피생장인자수체( EGFR)화혈관내피생장인자(VEGF)적표체정황.결과 신타미신자격조MCF-7세포적천이능력교미자격조명현강저；신타미신가정시간-제량의뢰성지억제MCF-7세포표면CXCR4적표체,병현저하조세포중CXCR4、COX-2화EGFR mRNA수평적표체,단대VEGF적산생병무명현적영향.결론MCF-7세포중CXCR4、COX-2화EGFR표체적현저하조,가능시신타미신억제MCF-7세포체외천이능력적중요궤제.
Objective To observe the effect of indomethacin on the migration of breast cancer cell line MCF-7 in vitro and investigate the mechanism involved.Methods The migration of MCF-7 cell line stimulated with or without indomethacin were tested using transwell plates consisting upper and lower chambers separated by Millipore polycarbonate membrance filters with 8 μm pore sizes; the levels of chemokine receptor 4(CXCR4),cyclooxygenase(COX-2),epidermal growth factor receptor(EGFR) and vascular endothelial growth factor(VEGF)expression in MCF-7 cell line were detected by flow cytometry,Real-time PCR and ELISA,respectively.Results Indomethacin decreased the migration ability of MCF-7 cell line significandy.CXCR4 membrane expression was significantly reduced in a time-dose dependent manner,and CXCR4,COX-2 and EGFR mRNA levels were significantly downregulated after indomethacin stimulation.However,exposure to indometahcin had no major effect on VEGF production of cells.Conclusion The downregulation of CXCR4,COX-2 and EGFR expression might be the primary mechanism involved in the inhibitory effect of indomethacin on the migration of MCF-7 cell line.