CAJ | 학술논문

目的研究选择性5-羟色胺2A(5-HT2A)受体拮抗剂沙格雷酯延缓大鼠移植动脉急性排斥反应后纤维化的作用。方法实验分为3组，同种移植对照组（供、受者分别为Wistar大鼠和SD大鼠）、同系移植对照组（供、受者均为SD大鼠）和实验组（供、受者分别为Wistar大鼠和SD大鼠），建立大鼠腹主动脉移植急性排斥反应后纤维化模型。术后各组大鼠喂养条件相同，仅实验组大鼠每天给予沙格雷酯灌胃，25 mg/kg。术后第14天和第60天对移植动脉行病理组织学及免疫组织化学检测，观察移植动脉内膜增生情况以及增殖细胞核抗原(PCNA)和平滑肌肌动蛋白(α-SMA)的表达情况。结果所有移植手术均获成功。术后第14天时，同种移植对照组移植动脉出现典型的急性排斥反应改变。术后第60天，同种移植对照组、同系移植对照组和实验组内膜指数分别为(62.41±6.54)％、(0.94±0.33)％和(16.71±3.94)％，3组间内膜指数的两两比较，差异均有统计学意义(P＜0.05); PCNA和α-SMA细胞阳性率分别为(0.99±0.54)％和(0.79±0.33)％、(22.43±3.40)％和(23.70±2.78)％及(7.37±4.61)％和(8.21±3.11)％，3组间PCNA阳性率的两两比较及α-SMA阳性率的两两比较，差异均有统计学意义(P＜0.05)。结论大鼠移植动脉急性排斥反应后可继发严重纤维化，沙格雷酯可显著延缓急性排斥反应后纤维化的发生、发展，其作用机制可能与下调PCNA和α-SMA的表达有关。
목적 연구선택성5-간색알2A(5-HT2A)수체길항제사격뢰지연완대서이식동맥급성배척반응후섬유화적작용。방법 실험분위3조，동충이식대조조（공、수자분별위Wistar대서화SD대서）、동계이식대조조（공、수자균위SD대서）화실험조（공、수자분별위Wistar대서화SD대서），건립대서복주동맥이식급성배척반응후섬유화모형。술후각조대서위양조건상동，부실험조대서매천급여사격뢰지관위，25 mg/kg。술후제14천화제60천대이식동맥행병리조직학급면역조직화학검측，관찰이식동맥내막증생정황이급증식세포핵항원(PCNA)화평활기기동단백(α-SMA)적표체정황。결과 소유이식수술균획성공。술후제14천시，동충이식대조조이식동맥출현전형적급성배척반응개변。술후제60천，동충이식대조조、동계이식대조조화실험조내막지수분별위(62.41±6.54)％、(0.94±0.33)％화(16.71±3.94)％，3조간내막지수적량량비교，차이균유통계학의의(P＜0.05); PCNA화α-SMA세포양성솔분별위(0.99±0.54)％화(0.79±0.33)％、(22.43±3.40)％화(23.70±2.78)％급(7.37±4.61)％화(8.21±3.11)％，3조간PCNA양성솔적량량비교급α-SMA양성솔적량량비교，차이균유통계학의의(P＜0.05)。결론 대서이식동맥급성배척반응후가계발엄중섬유화，사격뢰지가현저연완급성배척반응후섬유화적발생、발전，기작용궤제가능여하조PCNA화α-SMA적표체유관。
Objective To investigate the effect of sarpogrelate, a specific 5-HT2A receptor blocker,on fibrosis past acute rejection of abdominal aortic allotransplantation in rats. Methods The rat models of abdominal aortic transplantation were divided into three groups: allograft control group (Wistar→ SD), isograft control group ( SD→ SD) and sarpogrelate-treated group (Wistar→ SD).Sarpogrelate-treated group received intragastric administration of sarpogrelate every day. The pathologic and immunohistochemical findings of the transplant aorta were observed at 14th and 60th day after transplantation. Results At 14th day, visible acute rejection could be observed in allograft control group,but not in isograft control group. At 60th day, vascular intimal index of sarpogrelatetreated group was significantly lower than that in allograft control group[( 16. 71 ± 3. 94)％ versus (62. 41 ± 6. 54)％ ,P＜0. 05). The expression of PCNA and α-SMA in sarpogrelate-treated group wasalso significantly lower than that in allograft control group[(7. 37 ± 4. 61)％ versus (22. 43 ±3.40)％,(8.21 ± 3. 11)％ versus. (23.70 ± 2.78)％, P＜0.05, respectively). Conclusion The expression of PCNA and α-SMA of the transplant aorta could be suppressed by sarpogrelate, and sarpogrelate could relieve the fibrosis past acute rejection of aortic allograft.